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In vitro cellular & developmental biology. Animal1995; 31(8); 574-578; doi: 10.1007/BF02634308

Canine and equine mesangial cells in vitro.

Abstract: No abstract available
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Publication Date: 1995-09-01 PubMed ID: 8528510DOI: 10.1007/BF02634308Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper outlines a study on canine and equine mesangial cells, specializing pericytes within kidney’s glomeruli, which are crucial in the development of glomerular diseases. This study seeks to elucidate the conditions necessary for the isolation and propagation of these cells in vitro, which would aid in glomerular disease research across different species.

Glomerular Disease

  • The study begins by highlighting the crucial role of the glomerular mesangium in the progression of chronic renal failure in domestic animals and humans.
  • This advance into the renal failure stage is characterized by the proliferation of mesangial cells (MC), as well as the synthesis of cytokines and the contraction of MC.

Study of Mesangial Cells

  • The researchers aim to develop a culture of MC in vitro, which offers a practical model for studying glomerular diseases.
  • They acknowledge studies with rodent, human, porcine, and bovine MC that have demonstrated significant species differences in MC biology, emphasizing the importance of using autologous cells in studying glomerular diseases.

The Canine and Equine Study

  • Canine and equine MC culture is presented as an alternative model for studying glomerular disease due to the ease of obtaining canine and equine kidneys, as well as the superior glomerular count compared to human renal biopsies or rodent kidneys.
  • This study involves the isolation of glomeruli from freshly harvested canine and equine kidneys, followed by culture and growth observation.

Characterizing Mesangial Cells

  • The researchers mention that the glomerulus contains multiple different cell types, one of which is the mesangial cells.
  • They also highlight the challenge in identifying MC grown in vitro due to the lack of a specific marker leading to a verification process through a method of exclusion and confirmation.
  • The characterization studies to validate the mesangial cell differentiation and culture purity were conducted using immunofluorescence and electron microscopy.

Cite This Article

APA
Ennulat D, Brown SA. (1995). Canine and equine mesangial cells in vitro. In Vitro Cell Dev Biol Anim, 31(8), 574-578. https://doi.org/10.1007/BF02634308

Publication

In vitro cellular & developmental biology. Animal
ISSN: 1071-2690
NlmUniqueID: 9418515
Country: Germany
Language: English
Volume: 31
Issue: 8
Pages: 574-578

Researcher Affiliations

Ennulat, D
    Brown, S A

      MeSH Terms

      • Animals
      • Cell Culture Techniques
      • Cell Division
      • Culture Media
      • Dogs
      • Glomerular Mesangium / cytology
      • Horses
      • Microfilament Proteins / metabolism

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      Citations

      This article has been cited 0 times.
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