Cartilage oligomeric matrix protein (COMP) levels in digital sheath synovial fluid and serum with tendon injury.
Abstract: Cartilage oligomeric matrix protein (COMP) is a noncollagenous extracellular matrix protein found predominantly in cartilage, but also in tendon, ligament and meniscus. Studies in man have demonstrated that it may be used as a prognostic marker in rheumatoid arthritis and osteoarthritis. The present study investigated whether tendon injury contributes to serum and tendon sheath synovial fluid levels of COMP in horses. COMP levels, analysed by competitive ELISA, in the digital sheath synovial fluid were more than 10-fold higher than in the serum. Levels were significantly raised when tendon damage or sepsis was present within the tendon sheath but showed only mild, statistically insignificant, elevation in cases of tenosynovitis alone. COMP concentrations in serum were found to vary with age. Foals (age < or = 1 year) had significantly (P<0.001) higher levels in comparison to older control horses. Total COMP concentrations in an age-matched group with tendinitis were not significantly different from the control group. Measurements of COMP levels in tendon sheath synovial fluid are therefore useful in depicting processes in tendon tissue, while elevated serum levels are likely to be more representative of joint disease than tendinitis.
Publication Date: 2000-02-08 PubMed ID: 10661386DOI: 10.2746/042516400777612053Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research focuses on the presence and variation of Cartilage Oligomeric Matrix Protein (COMP) in the serum and synovial fluid within the tendon sheath of horses, and its potential as an indicative marker for tendon injury or disease.
Research Context and Aim
- The protein molecule COMP, found largely in cartilage and other connecting tissues in organisms, has been proven useful in the medical field as a prognosis tool for diseases such as rheumatoid arthritis and osteoarthritis.
- While COMP is not typically associated with tendons, its role and detected change of levels in tendon injury marks an avenue yet to be explored fully.
- This research aims to investigate the contribution of tendon injury to levels of COMP in the serum and synovial fluid within the digital sheath of horses.
Research Methodology and Findings
- COMP levels in the fluid were tested using competitive ELISA, a scientific technique for detecting the presence of bio-compounds.
- The study discovered that COMP levels in the fluid were significantly higher by over 10 times than in the serum.
- The research also found marked elevation in COMP concentration when tendon damage or sepsis (infection) was present within the tendon sheath.
- In comparison, there was only a minor, statistically trivial rise in COMP levels in instances of tenosynovitis alone, a condition characterized by the inflammation of the synovial sheath of tendons.
Relation with Age and Tendon Conditions
- Another important finding was that COMP concentrations in the bloodstream varied with the age of the animal.
- For instance, foals of age ≤ 1 had substantially higher COMP levels compared to older horses.
- No significant difference was observed in COMP levels between the control group and a group with tendinitis matched by age.
Conclusion and Potential Applications
- The results of the research suggest that measuring COMP levels in the synovial fluid can offer insights into processes occurring within the tendon tissue.
- Contrastingly, elevated serum levels of COMP are probably more indicative of joint disease rather than tendonitis.
- These conclusions have potential applications in veterinary medicine for early disease detection and prognosis.
Cite This Article
APA
Smith RK, Heinegård D.
(2000).
Cartilage oligomeric matrix protein (COMP) levels in digital sheath synovial fluid and serum with tendon injury.
Equine Vet J, 32(1), 52-58.
https://doi.org/10.2746/042516400777612053 Publication
Researcher Affiliations
- Department of Farm Animal and Equine Medicine and Surgery, The Royal Veterinary College, Hatfield, Herts, UK.
MeSH Terms
- Age Factors
- Animals
- Blotting, Western / veterinary
- Case-Control Studies
- Enzyme-Linked Immunosorbent Assay / veterinary
- Extracellular Matrix Proteins / analysis
- Extracellular Matrix Proteins / blood
- Extracellular Matrix Proteins / immunology
- Glycoproteins / analysis
- Glycoproteins / blood
- Glycoproteins / immunology
- Horse Diseases / diagnosis
- Horse Diseases / metabolism
- Horses
- Joint Diseases / diagnosis
- Joint Diseases / metabolism
- Joint Diseases / veterinary
- Matrilin Proteins
- Synovial Fluid / chemistry
- Synovial Fluid / immunology
- Tendinopathy / diagnosis
- Tendinopathy / metabolism
- Tendinopathy / veterinary
- Tendon Injuries / diagnosis
- Tendon Injuries / metabolism
- Tendon Injuries / veterinary
Citations
This article has been cited 9 times.- Ma M, Liang X, Wang X, Zhang L, Cheng S, Guo X, Zhang F, Wen Y. The molecular mechanism study of COMP involved in the articular cartilage damage of Kashin-Beck disease.. Bone Joint Res 2020 Sep;9(9):578-586.
- Smith R, Önnerfjord P, Holmgren K, di Grado S, Dudhia J. Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease.. Int J Mol Sci 2020 Mar 20;21(6).
- Ekman S, Lindahl A, Rüetschi U, Jansson A, Björkman K, Abrahamsson-Aurell K, Björnsdóttir S, Löfgren M, Hultén LM, Skiöldebrand E. Effect of circadian rhythm, age, training and acute lameness on serum concentrations of cartilage oligomeric matrix protein (COMP) neo-epitope in horses.. Equine Vet J 2019 Sep;51(5):674-680.
- Georgiev T, Ivanova M, Kopchev A, Velikova T, Miloshov A, Kurteva E, Yuzeir K, Penkov M, Kabakchieva P, Rashkov R, Stoilov R. Cartilage oligomeric protein, matrix metalloproteinase-3, and Coll2-1 as serum biomarkers in knee osteoarthritis: a cross-sectional study.. Rheumatol Int 2018 May;38(5):821-830.
- Lei Q, Liu H, Peng Y, Xiao P. In silico target fishing and pharmacological profiling for the isoquinoline alkaloids of Macleaya cordata (Bo Luo Hui).. Chin Med 2015;10:37.
- Furman BD, Mangiapani DS, Zeitler E, Bailey KN, Horne PH, Huebner JL, Kraus VB, Guilak F, Olson SA. Targeting pro-inflammatory cytokines following joint injury: acute intra-articular inhibition of interleukin-1 following knee injury prevents post-traumatic arthritis.. Arthritis Res Ther 2014 Jun 25;16(3):R134.
- Dakin SG, Dudhia J, Smith RK. Resolving an inflammatory concept: the importance of inflammation and resolution in tendinopathy.. Vet Immunol Immunopathol 2014 Apr 15;158(3-4):121-7.
- Dakin SG, Smith RK, Heinegård D, Önnerfjord P, Khabut A, Dudhia J. Proteomic analysis of tendon extracellular matrix reveals disease stage-specific fragmentation and differential cleavage of COMP (cartilage oligomeric matrix protein).. J Biol Chem 2014 Feb 21;289(8):4919-27.
- Siebuhr AS, Wang J, Karsdal M, Bay-Jensen AC, Y J, Q Z. Matrix metalloproteinase-dependent turnover of cartilage, synovial membrane, and connective tissue is elevated in rats with collagen induced arthritis.. J Transl Med 2012 Sep 20;10:195.
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