Centrifugation and addition of glycerol at 22 degres C instead of 4 degrees C improve post-thaw motility and fertility of stallion spermatozoa.

The research study investigates the impact of temperature at the point of centrifugation and glycerol-addition on the mobility and fertility of frozen stallion sperm post-thaw. The results reveal that the application of these processes at 22 degrees C, followed by moderate cooling, improves post-thaw mobility, recovery rate, and fertility.

Objective of the Study

• This study aims to test how the rate of cooling to 4 degrees Celsius and the temperature during the centrifugation/glycerol-introduction phase influence recovery rates after centrifugation, motility after thawing, and fertility per cycle.

Methodology and Findings

• When the processes of centrifugation and glycerol-introduction were performed at a temperature of 4 degrees Celsius using 14 samples, the study found that a moderate rate of cooling yielded higher motility after thawing compared to a slow cooling rate.
• In another experiment, 10 samples were treated at 22 degrees Celsius. Post-thaw motility was lower when the sperm was frozen directly from 22 degrees Celsius than when the sperm was cooled to 4 degrees Celsius before freezing.
• Remarkably, when centrifugation and glycerol-introduction were executed at 22 degrees Celsius, followed by cooling at a moderate rate, researchers witnessed an improvement in the recovery rate of spermatozoa after centrifugation, motility of the spermatozoa after thawing, and fertility per cycle.

Conclusion

• From the research findings, it is clear that executing the processes of centrifugation and glycerol-addition at 22 degrees Celsius, then cooling down moderately to 4 degrees Celsius, leads to improvement in the motility of sperm after thawing, the recovery rate of sperm post-centrifugation, and fertility per cycle.