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Home / Equine Research Bank / Biology (Basel) / Centrifugation Force and Time Alter CASA Parameters and Oxid...
Biology2020; 9(2); doi: 10.3390/biology9020022

Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm.

Abstract: Conventional sperm selection techniques used in ARTs rely on centrifugation steps. To date, the different studies reported on the effects of centrifugation on stallion sperm motility provided contrasting results and do not include effects on mitochondrial functionality and different oxidative parameters. The effects of different centrifugation protocols (300 ×g for 5', 300 ×g for 10', 1500 ×g for 5' and 1500 ×g for 10' vs no centrifugation) on motility and oxidative status in cryopreserved stallion sperm, were analyzed. After centrifugation, almost all motility parameters were significantly altered, as observed by computer-assisted sperm analysis. A polarographic assay of oxygen consumption showed a progressive decrease in mitochondria respiration from the gentlest to the strongest protocol. By laser scanning confocal microscopy, significant reduction of mitochondrial membrane potential, at any tested protocol, and time-dependent effects, at the same centrifugal force, were found. Increased DNA fragmentation index at any tested protocol and time-dependent effects at the same centrifugal force were found, whereas increased protein carbonylation was observed only at the strongest centrifugal force. These results provide more comprehensive understandings on centrifugation-induced effects on cryopreserved stallion sperm and suggest that, even at a weak force for a short time, centrifugation impairs different aspects of equine sperm metabolism and functionality.
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Publication Date: 2020-01-27 PubMed ID: 32012799PubMed Central: PMC7168157DOI: 10.3390/biology9020022Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines how different centrifugation forces and times impact the movement and oxidative status of frozen stallion sperm. It found that changes in these methods can significantly impact various aspects of sperm movement and functionality.

Research Overview

The research focuses on understanding the effects of different centrifugation protocols on the movement and oxidative status of frozen stallion sperm. These protocols varied in terms of centrifugal force and time (300 ×g for 5′, 300 ×g for 10′, 1500 ×g for 5′ and 1500 ×g for 10′). The results were then compared to a set where centrifugation was not used.

Findings and Observations

  • After centrifugation, almost all motility parameters were markedly changed, as noted by a computer-assisted sperm analysis.
  • A test observing oxygen consumption showed a gradual decline in mitochondrial respiration from the least forceful to the most forceful protocol.
  • A significant reduction of mitochondrial membrane potential was observed in all tested protocols through laser scanning confocal microscopy. Additionally, time-dependent effects at the same centrifugal force were noted.
  • Increased DNA fragmentation index was observed at any tested protocol and time-dependent effects at the same centrifugal force were noted.
  • Increased protein carbonylation was only observed at the highest centrifugal force used.

Conclusions and Implications

These findings provide a broader understanding of centrifugation-induced effects on frozen stallion sperm. The various parameters – motility, mitochondrial respiration, mitochondrial membrane potential, DNA fragmentation index, and protein carbonylation – provide insights into the health and functionality of the sperm. The research suggests that even at low force for a short time, centrifugation can impair different aspects of equine sperm metabolism and functionality. This understanding could potentially influence the techniques used in Assisted Reproductive Technologies (ARTs) and improve the success rate of these procedures.

Cite This Article

APA
Marzano G, Moscatelli N, Di Giacomo M, Martino NA, Lacalandra GM, Dell'Aquila ME, Maruccio G, Primiceri E, Chiriacò MS, Zara V, Ferramosca A. (2020). Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm. Biology (Basel), 9(2). https://doi.org/10.3390/biology9020022

Publication

Biology
ISSN: 2079-7737
NlmUniqueID: 101587988
Country: Switzerland
Language: English
Volume: 9
Issue: 2

Researcher Affiliations

Marzano, Giuseppina
  • Department of Mathematics and Physics E. de Giorgi, University of Salento, Via per Arnesano, 73100 Lecce, Italy.
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
  • Scuola Superiore ISUFI (Istituto Superiore Universitario di Formazione Interdisciplinare), University of Salento, Via per Arnesano, 73100 Lecce, Italy.
Moscatelli, Natalina
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.
  • Center for Biomolecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, Via Barsanti, I-73010 Arnesano, Lecce, Italy.
Di Giacomo, Mariangela
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.
Martino, Nicola Antonio
  • Department of Biosciences, Biotechnologies & Biopharmaceutics, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy.
  • Department of Veterinary Sciences, University of Torino, Largo Paolo Braccini, 10095 Grugliasco, Torino, Italy.
Lacalandra, Giovanni Michele
  • Department of Veterinary Medicine, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy.
Dell'Aquila, Maria Elena
  • Department of Biosciences, Biotechnologies & Biopharmaceutics, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy.
Maruccio, Giuseppe
  • Department of Mathematics and Physics E. de Giorgi, University of Salento, Via per Arnesano, 73100 Lecce, Italy.
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
Primiceri, Elisabetta
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
Chiriacò, Maria Serena
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
Zara, Vincenzo
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.
Ferramosca, Alessandra
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.

Grant Funding

  • PON FSE - FESR 2014-2020 (CCI 2014IT16M2OP005) - Axis I "Investments in Human Capital" Action I.1 "Innovative PhDs with industrial characterization" - project DOT1712250 code 2 / Ministero dell'Istruzione, dell'Università e della Ricerca

Conflict of Interest Statement

The authors declare no conflict of interest.

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