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Biology2020; 9(2); doi: 10.3390/biology9020022

Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm.

Abstract: Conventional sperm selection techniques used in ARTs rely on centrifugation steps. To date, the different studies reported on the effects of centrifugation on stallion sperm motility provided contrasting results and do not include effects on mitochondrial functionality and different oxidative parameters. The effects of different centrifugation protocols (300 ×g for 5', 300 ×g for 10', 1500 ×g for 5' and 1500 ×g for 10' vs no centrifugation) on motility and oxidative status in cryopreserved stallion sperm, were analyzed. After centrifugation, almost all motility parameters were significantly altered, as observed by computer-assisted sperm analysis. A polarographic assay of oxygen consumption showed a progressive decrease in mitochondria respiration from the gentlest to the strongest protocol. By laser scanning confocal microscopy, significant reduction of mitochondrial membrane potential, at any tested protocol, and time-dependent effects, at the same centrifugal force, were found. Increased DNA fragmentation index at any tested protocol and time-dependent effects at the same centrifugal force were found, whereas increased protein carbonylation was observed only at the strongest centrifugal force. These results provide more comprehensive understandings on centrifugation-induced effects on cryopreserved stallion sperm and suggest that, even at a weak force for a short time, centrifugation impairs different aspects of equine sperm metabolism and functionality.
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Publication Date: 2020-01-27 PubMed ID: 32012799PubMed Central: PMC7168157DOI: 10.3390/biology9020022Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study examines how different centrifugation forces and times impact the movement and oxidative status of frozen stallion sperm. It found that changes in these methods can significantly impact various aspects of sperm movement and functionality.

Research Overview

The research focuses on understanding the effects of different centrifugation protocols on the movement and oxidative status of frozen stallion sperm. These protocols varied in terms of centrifugal force and time (300 ×g for 5′, 300 ×g for 10′, 1500 ×g for 5′ and 1500 ×g for 10′). The results were then compared to a set where centrifugation was not used.

Findings and Observations

  • After centrifugation, almost all motility parameters were markedly changed, as noted by a computer-assisted sperm analysis.
  • A test observing oxygen consumption showed a gradual decline in mitochondrial respiration from the least forceful to the most forceful protocol.
  • A significant reduction of mitochondrial membrane potential was observed in all tested protocols through laser scanning confocal microscopy. Additionally, time-dependent effects at the same centrifugal force were noted.
  • Increased DNA fragmentation index was observed at any tested protocol and time-dependent effects at the same centrifugal force were noted.
  • Increased protein carbonylation was only observed at the highest centrifugal force used.

Conclusions and Implications

These findings provide a broader understanding of centrifugation-induced effects on frozen stallion sperm. The various parameters – motility, mitochondrial respiration, mitochondrial membrane potential, DNA fragmentation index, and protein carbonylation – provide insights into the health and functionality of the sperm. The research suggests that even at low force for a short time, centrifugation can impair different aspects of equine sperm metabolism and functionality. This understanding could potentially influence the techniques used in Assisted Reproductive Technologies (ARTs) and improve the success rate of these procedures.

Cite This Article

APA
Marzano G, Moscatelli N, Di Giacomo M, Martino NA, Lacalandra GM, Dell'Aquila ME, Maruccio G, Primiceri E, Chiriacò MS, Zara V, Ferramosca A. (2020). Centrifugation Force and Time Alter CASA Parameters and Oxidative Status of Cryopreserved Stallion Sperm. Biology (Basel), 9(2). https://doi.org/10.3390/biology9020022

Publication

Biology
ISSN: 2079-7737
NlmUniqueID: 101587988
Country: Switzerland
Language: English
Volume: 9
Issue: 2

Researcher Affiliations

Marzano, Giuseppina
  • Department of Mathematics and Physics E. de Giorgi, University of Salento, Via per Arnesano, 73100 Lecce, Italy.
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
  • Scuola Superiore ISUFI (Istituto Superiore Universitario di Formazione Interdisciplinare), University of Salento, Via per Arnesano, 73100 Lecce, Italy.
Moscatelli, Natalina
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.
  • Center for Biomolecular Nanotechnologies @UNILE, Istituto Italiano di Tecnologia, Via Barsanti, I-73010 Arnesano, Lecce, Italy.
Di Giacomo, Mariangela
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.
Martino, Nicola Antonio
  • Department of Biosciences, Biotechnologies & Biopharmaceutics, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy.
  • Department of Veterinary Sciences, University of Torino, Largo Paolo Braccini, 10095 Grugliasco, Torino, Italy.
Lacalandra, Giovanni Michele
  • Department of Veterinary Medicine, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy.
Dell'Aquila, Maria Elena
  • Department of Biosciences, Biotechnologies & Biopharmaceutics, University of Bari Aldo Moro, km3 Strada per Casamassima, 70100 Valenzano, Bari, Italy.
Maruccio, Giuseppe
  • Department of Mathematics and Physics E. de Giorgi, University of Salento, Via per Arnesano, 73100 Lecce, Italy.
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
Primiceri, Elisabetta
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
Chiriacò, Maria Serena
  • Institute of Nanotechnology, CNR NANOTEC, Via per Monteroni, 73100 Lecce, Italy.
Zara, Vincenzo
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.
Ferramosca, Alessandra
  • Department of Biological and Environmental Sciences and Technologies, University of Salento, Via per Monteroni, 73100 Lecce, Italy.

Grant Funding

  • PON FSE - FESR 2014-2020 (CCI 2014IT16M2OP005) - Axis I "Investments in Human Capital" Action I.1 "Innovative PhDs with industrial characterization" - project DOT1712250 code 2 / Ministero dell'Istruzione, dell'Università e della Ricerca

Conflict of Interest Statement

The authors declare no conflict of interest.

References

This article includes 61 references
  1. Henkel R.R., Schill W.B.. Sperm Preparation for Art. Reprod. Biol. Endocrinol. 2003;1:108.
    doi: 10.1186/1477-7827-1-108pmc: PMC293422pubmed: 14617368google scholar: lookup
  2. . Laboratory Manual Examination and Processing of Human Semen. WHO 2010.
  3. Verma O.P., Kumar R., Kumar A., Chand S.. Assisted Repproductive Techniques in Farm Animal - from Artificial Insemination to Nanobiotechnology. Vet. World 2012;5:301–310.
    doi: 10.5455/vetworld.2012.301-310google scholar: lookup
  4. Li Z., Zhou Y., Liu R., Lin H., Lin W., Xiao W., Lin Q.. Effects of Semen Processing on the Generation of Reactive Oxygen Species and Mitochondrial Membrane Potential of Human Spermatozoa. Andrologia 2012;44:157–163.
    doi: 10.1111/j.1439-0272.2010.01123.xpubmed: 21729130google scholar: lookup
  5. Alvarez J.G., Lasso J.L., Blasco L., Nunez R.C., Heyner S., Caballero P.P., Storey B.T.. Centrifugation of Human Spermatozoa Induces Sublethal Damage; Separation of Human Spermatozoa from Seminal Plasma by a Dextran Swim-up Procedure without Centrifugation Extends Their Motile Lifetime. Hum. Reprod. 1993;8:1087–1092.
    doi: 10.1093/oxfordjournals.humrep.a138198pubmed: 7691866google scholar: lookup
  6. Zhu W.J.. Preparation and Observation Methods Can Produce Misleading Artefacts in Human Sperm Ultrastructural Morphology. Andrologia 2018:50.
    doi: 10.1111/and.13043pubmed: 29732590google scholar: lookup
  7. Ghaleno L.R., Valojerdi M.R., Janzamin E., Chehrazi M., Sharbatoghli M., Yazdi R.S.. Evaluation of Conventional Semen Parameters, Intracellular Reactive Oxygen Species, DNA Fragmentation and Dysfunction of Mitochondrial Membrane Potential after Semen Preparation Techniques: A Flow Cytometric Study. Arch. Gynecol. Obs. 2014;289:173–180.
    doi: 10.1007/s00404-013-2946-1pubmed: 23846620google scholar: lookup
  8. Muratori M., Tarozzi N., Cambi M., Boni L., Iorio A.L., Passaro C., Luppino B., Nadalini M., Marchiani S., Tamburrino L.. Variation of DNA Fragmentation Levels During Density Gradient Sperm Selection for Assisted Reproduction Techniques: A Possible New Male Predictive Parameter of Pregnancy?. Medicine (Baltim.) 2016;95:e3624.
    doi: 10.1097/MD.0000000000003624pmc: PMC4902407pubmed: 27196465google scholar: lookup
  9. Oliveira L.Z., Arruda R.P., Celeghini E.C.C., de Andrade A.F.C., Perini A.P., Resende M.V., Miguel M.C.V., Lucio A.C., Hossepian de Lima V.F.M.. Effects of Discontinuous Percoll Gradient Centrifugation on the Quality of Bovine Spermatozoa Evaluated with Computer-Assisted Semen Analysis and Fluorescent Probes Association. Andrologia 2012;44:9–15.
    doi: 10.1111/j.1439-0272.2010.01096.xpubmed: 21615453google scholar: lookup
  10. Varisli O., Uguz C., Agca C., Agca Y.. Various Physical Stress Factors on Rat Sperm Motility, Integrity of Acrosome, and Plasma Membrane. J. Androl. 2009;30:75–86.
    doi: 10.2164/jandrol.107.004333pubmed: 18723472google scholar: lookup
  11. Kim S., Agca C., Agca Y.. Effects of Various Physical Stress Factors on Mitochondrial Function and Reactive Oxygen Species in Rat Spermatozoa. Reprod. Fertil. Dev. 2013;25:1051–1064.
    doi: 10.1071/RD12212pmc: PMC3706545pubmed: 23140582google scholar: lookup
  12. Ambruosi B., Lacalandra G.M., Iorga A.I., De Santis T., Mugnier S., Matarrese R., Goudet G., Dell’aquila M.E.. Cytoplasmic Lipid Droplets and Mitochondrial Distribution in Equine Oocytes: Implications on Oocyte Maturation, Fertilization and Developmental Competence after Icsi. Theriogenology 2009;71:1093–1104.
    doi: 10.1016/j.theriogenology.2008.12.002pubmed: 19167745google scholar: lookup
  13. Hinrichs K.. Assisted Reproductive Techniques in Mares. Reprod. Domest. Anim. 2018;53:4–13.
    doi: 10.1111/rda.13259pubmed: 30238661google scholar: lookup
  14. Leemans B., Stout T.A.E., De Schauwer C., Heras S., Nelis H., Hoogewijs M., Van Soom A., Gadella B.M.. Update on Mammalian Sperm Capacitation: How Much Does the Horse Differ from Other Species?. Reproduction 2019;157:R181.
    doi: 10.1530/REP-18-0541pubmed: 30721132google scholar: lookup
  15. England G.. Fertility and Obstetrics in the Horse. 2008. pp. 200–211.
  16. McKinnon A.O., Squires E.L., Vaala W.E., Varner D.D.. Equine Reproduction. 2011. pp. 867–1571.
  17. Neuhauser S., Gosele P., Handler J.. Postthaw Addition of Autologous Seminal Plasma Improves Sperm Motion Characteristics in Fair and Poor Freezer Stallions. J. Equine Vet. Sci. 2019;72:117–123.
    doi: 10.1016/j.jevs.2018.10.028pubmed: 30929775google scholar: lookup
  18. Pena F.J., Garcia B.M., Samper J.C., Aparicio I.M., Tapia J.A., Ferrusola C.O.. Dissecting the Molecular Damage to Stallion Spermatozoa: The Way to Improve Current Cryopreservation Protocols?. Theriogenology 2011;76:1177–1186.
    doi: 10.1016/j.theriogenology.2011.06.023pubmed: 21835453google scholar: lookup
  19. Gibb Z., Aitken R.J.. Recent Developments in Stallion Semen Preservation. J. Equine Vet. Sci. 2016;43:S29–S36.
    doi: 10.1016/j.jevs.2016.06.006google scholar: lookup
  20. Sieme H., Harrison R.A., Petrunkina A.M.. Cryobiological Determinants of Frozen Semen Quality, with Special Reference to Stallion. Anim. Reprod. Sci. 2008;107:276–292.
    doi: 10.1016/j.anireprosci.2008.05.001pubmed: 18585878google scholar: lookup
  21. Loomis P.R., Graham J.K.. Commercial Semen Freezing: Individual Male Variation in Cryosurvival and the Response of Stallion Sperm to Customized Freezing Protocols. Anim. Reprod. Sci. 2008;105:119–128.
    doi: 10.1016/j.anireprosci.2007.11.010pubmed: 18178040google scholar: lookup
  22. Papas M., Catalan J., Fernandez-Fuertes B., Arroyo L., Bassols A., Miro J., Yeste M.. Specific Activity of Superoxide Dismutase in Stallion Seminal Plasma Is Related to Sperm Cryotolerance. Antioxidants (Basel) 2019;8:539.
    doi: 10.3390/antiox8110539pmc: PMC6912747pubmed: 31717586google scholar: lookup
  23. Yeste M., Estrada E., Rocha L.G., Marin H., Rodriguez-Gil J.E., Miro J.. Cryotolerance of Stallion Spermatozoa Is Related to Ros Production and Mitochondrial Membrane Potential Rather Than to the Integrity of Sperm Nucleus. Andrology 2015;3:395–407.
    doi: 10.1111/andr.291pubmed: 25294093google scholar: lookup
  24. Pickett B.W., Sullivan J.J., Byers W.W., Pace M.M., Remmenga E.E.. Effect of Centrifugation and Seminal Plasma on Motility and Fertility of Stallion and Bull Spermatozoa. Fertil. Steril. 1975;26:167–174.
    doi: 10.1016/S0015-0282(16)40938-6pubmed: 1126461google scholar: lookup
  25. Jasko D.J., Moran D.M., Farlin M.E., Squires E.L.. Effect of Seminal Plasma Dilution or Removal on Spermatozoal Motion Characteristics of Cooled Stallion Semen. Theriogenology 1991;35:1059–1067.
    doi: 10.1016/0093-691X(91)90354-Ggoogle scholar: lookup
  26. Brinsko S.P., Crockett E.C., Squires E.L.. Effect of Centrifugation and Partial Removal of Seminal Plasma on Equine Spermatozoal Motility after Cooling and Storage. Theriogenology 2000;54:129–136.
    doi: 10.1016/S0093-691X(00)00331-9pubmed: 10990354google scholar: lookup
  27. Kareskoski A.M., Reilas T., Andersson M., Katila T.. Motility and Plasma Membrane Integrity of Spermatozoa in Fractionated Stallion Ejaculates after Storage. Reprod. Domest. Anim. 2006;41:33–38.
    doi: 10.1111/j.1439-0531.2006.00647.xpubmed: 16420325google scholar: lookup
  28. Maarten H., Rijsselaere T., de Vliegher S., Vanhaesebrouck E., de Schauwer C., Jan Govaere Thys M., Hoflack G., van Soom A., de Kruif A.. Influence of Different Centrifugation Protocols on Equine Semen Preservation. Theriogenology 2010;74:118–126.
    pubmed: 20207406
  29. Len J.A., Jenkins J.A., Eilts B.E., Paccamonti D.L., Lyle S.K., Hosgood G.. Immediate and Delayed (after Cooling) Effects of Centrifugation on Equine Sperm. Theriogenology 2010;73:225–231.
    doi: 10.1016/j.theriogenology.2009.09.003pubmed: 19913898google scholar: lookup
  30. Len J.A., Beehan D.P., Lyle S.K., Eilts B.E.. Cushioned Versus Noncushioned Centrifugation: Sperm Recovery Rate and Integrity. Theriogenology 2013;80:648–653.
    doi: 10.1016/j.theriogenology.2013.06.009pubmed: 23849256google scholar: lookup
  31. Piomboni P., Focarelli R., Stendardi A., Ferramosca A., Zara V.. The Role of Mitochondria in Energy Production for Human Sperm Motility. Int. J. Androl. 2012;35:109–124.
    doi: 10.1111/j.1365-2605.2011.01218.xpubmed: 21950496google scholar: lookup
  32. Ferramosca A., Zara V.. Bioenergetics of Mammalian Sperm Capacitation. Biomed. Res. Int. 2014;2014.
    doi: 10.1155/2014/902953pmc: PMC3984864pubmed: 24791005google scholar: lookup
  33. Moscatelli N., Spagnolo B., Pisanello M., Lemma E.D., de Vittorio M., Zara V., Pisanello F., Ferramosca A.. Single-Cell-Based Evaluation of Sperm Progressive Motility Via Fluorescent Assessment of Mitochondria Membrane Potential. Sci. Rep. 2017;7:10.
    doi: 10.1038/s41598-017-18123-1pmc: PMC5738389pubmed: 29263401google scholar: lookup
  34. Natalina M., Lunetti P., Braccia C., Armirotti A., Pisanello F., de Vittorio M., Zara V., Ferramosca A.. Comparative Proteomic Analysis of Proteins Involved in Bioenergetics Pathways Associated with Human Sperm Motility. Int. J. Mol. Sci. 2019;20:3000.
    pmc: PMC6627292pubmed: 31248186
  35. Saraste M.. Oxidative Phosphorylation at the Fin De Siecle. Science 1999;283:1488–1493.
    doi: 10.1126/science.283.5407.1488pubmed: 10066163google scholar: lookup
  36. Gibb Z., Sarah R.L., Robert J.A.. The Paradoxical Relationship between Stallion Fertility and Oxidative Stress1. Biol. Reprod. 2014;91.
    doi: 10.1095/biolreprod.114.118539pubmed: 25078685google scholar: lookup
  37. Moraes C.R., Meyers S.. The Sperm Mitochondrion: Organelle of Many Functions. Anim. Reprod. Sci. 2018;194:71–80.
    doi: 10.1016/j.anireprosci.2018.03.024pubmed: 29605167google scholar: lookup
  38. Pena F.J., O’Flaherty C., Rodriguez J.M.O., Cano F.E.M., Gaitskell-Phillips G.L., Gil M.C., Ferrusola C.O.. Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa. Antioxidants (Basel) 2019;8:567.
    doi: 10.3390/antiox8110567pmc: PMC6912273pubmed: 31752408google scholar: lookup
  39. Alessandra F., Provenzano S.P., Montagna D.D., Coppola L., Zara V.. Oxidative Stress Negatively Affects Human Sperm Mitochondrial Respiration. Urology 2013;82:78–83.
    pubmed: 23806394
  40. Aitken R.J., Gibb Z., Baker M.A., Drevet J., Gharagozloo P.. Causes and Consequences of Oxidative Stress in Spermatozoa. Reprod. Fertil. Dev. 2016;28:1–10.
    doi: 10.1071/RD15325pubmed: 27062870google scholar: lookup
  41. Lone S.A., Mohanty T.K., Baithalu R.K., Yadav H.P.. Sperm Protein Carbonylation. Andrologia 2019;51:e13233.
    doi: 10.1111/and.13233pubmed: 30637798google scholar: lookup
  42. Yves M., Dale B., Cohen M.. DNA Damage and Repair in Human Oocytes and Embryos: A Review. Zygote 2010;18:357–365.
    pubmed: 20663262
  43. Choi Y.H., Chung Y.G., Walker S.C., Westhusin M.E., Hinrichs K.. In Vitro Development of Equine Nuclear Transfer Embryos: Effects of Oocyte Maturation Media and Amino Acid Composition During Embryo Culture. Zygote 2003;11:77–86.
    doi: 10.1017/S0967199403001102pubmed: 12625532google scholar: lookup
  44. Minervini F., Lacalandra G.M., Filannino A., Garbetta A., Nicassio M., Dell’Aquila M.E., Visconti A.. Toxic Effects Induced by Mycotoxin Fumonisin B1 on Equine Spermatozoa: Assessment of Viability, Sperm Chromatin Structure Stability, Ros Production and Motility. Toxicology 2010;24:2072–2078.
    doi: 10.1016/j.tiv.2010.05.024pubmed: 20541003google scholar: lookup
  45. Ferramosca A., Focarelli R., Piomboni P., Coppola L., Zara V.. Oxygen Uptake by Mitochondria in Demembranated Human Spermatozoa: A Reliable Tool for the Evaluation of Sperm Respiratory Efficiency. Int. J. Androl. 2008;31:337–345.
    doi: 10.1111/j.1365-2605.2007.00775.xpubmed: 17573845google scholar: lookup
  46. Amaral A., Ramalho-Santos J.. Assessment of Mitochondrial Potential: Implications for the Correct Monitoring of Human Sperm Function. Int. J. Androl. 2010;33:E180–E186.
    doi: 10.1111/j.1365-2605.2009.00987.xpubmed: 19751363google scholar: lookup
  47. De Riccardis L., Rizzello A., Ferramosca A., Urso E., de Robertis F., Danieli A., Giudetti A.M., Trianni G., Zara V., Maffia M.. Bioenergetics Profile of Cd4(+) T Cells in Relapsing Remitting Multiple Sclerosis Subjects. J. Biotechnol. 2015;202:31–39.
    doi: 10.1016/j.jbiotec.2015.02.015pubmed: 25701681google scholar: lookup
  48. Giuseppina M., Chiriacò M.S., Primiceri E., Dell’Aquila M.E., Ramalho-Santos J., Zara V., Ferramosca A., Maruccio G.. Sperm Selection in Assisted Reproduction: A Review of Established Methods and Cutting-Edge Possibilities. Biotechnol. Adv. 2019.
    doi: 10.1016/j.biotechadv.2019.107498pubmed: 31836499google scholar: lookup
  49. Marzano G., Mastrorocco A., Zianni R., Mangiacotti M., Chiaravalle A.E., Lacalandra G.M., Minervini F., Cardinali A., Macciocca M., Vicenti R.. Altered Morphokinetics in Equine Embryos from Oocytes Exposed to Dehp During Ivm. Mol. Reprod. Dev. 2019;86:1388–1404.
    doi: 10.1002/mrd.23156pubmed: 31025442google scholar: lookup
  50. Hammerstedt R.H., Graham J.K., Nolan J.P.. Cryopreservation of Mammalian Sperm: What We Ask Them to Survive. J. Androl. 1990;11:73–88.
    pubmed: 2179184
  51. Darr C.R., Cortopassi G.A., Datta S., Varner D.D., Meyers S.A.. Mitochondrial Oxygen Consumption Is a Unique Indicator of Stallion Spermatozoal Health and Varies with Cryopreservation Media. Theriogenology 2016;86:1382–1392.
    doi: 10.1016/j.theriogenology.2016.04.082pubmed: 27242178google scholar: lookup
  52. Darr C.R., Moraes L.E., Scanlan T.N., Baumber-Skaife J., Loomis P.R., Cortopassi G.A., Meyers S.A.. Sperm Mitochondrial Function Is Affected by Stallion Age and Predicts Post-Thaw Motility. J. Equine Vet. Sci. 2017;50:52–61.
    doi: 10.1016/j.jevs.2016.10.015google scholar: lookup
  53. Magdanz V., Boryshpolets S., Ridzewski C., Eckel B., Reinhardt K.. The Motility-Based Swim-up Technique Separates Bull Sperm Based on Differences in Metabolic Rates and Tail Length. PLoS ONE 2019;14.
    doi: 10.1371/journal.pone.0223576pmc: PMC6786571pubmed: 31600297google scholar: lookup
  54. Calvert S.J., Reynolds S., Paley M.N., Walters S.J., Pacey A.A.. Probing Human Sperm Metabolism Using 13c-Magnetic Resonance Spectroscopy. Mol. Hum. Reprod. 2019;25:30–41.
    doi: 10.1093/molehr/gay046pmc: PMC6314230pubmed: 30395244google scholar: lookup
  55. Gravance C.G., Garner D.L., Baumber J., Ball B.A.. Assessment of Equine Sperm Mitochondrial Function Using Jc-1. Theriogenology 2000;53:1691–1703.
    doi: 10.1016/S0093-691X(00)00308-3pubmed: 10968415google scholar: lookup
  56. Morrell J.M., Lagerqvist A., Humblot P., Johannisson A.. Effect of Single Layer Centrifugation on Reactive Oxygen Species and Sperm Mitochondrial Membrane Potential in Cooled Stallion Semen. Reprod. Fertil. Dev. 2017;29:1039–1045.
    doi: 10.1071/RD15440pubmed: 27048867google scholar: lookup
  57. Pena F.J., Ball B.A., Squires E.L.. A New Method for Evaluating Stallion Sperm Viability and Mitochondrial Membrane Potential in Fixed Semen Samples. Cytom. B Clin. Cytom. 2018;94:302–311.
    doi: 10.1002/cyto.b.21506pubmed: 28033647google scholar: lookup
  58. Jezek J., Cooper K.F., Strich R.. Reactive Oxygen Species and Mitochondrial Dynamics: The Yin and Yang of Mitochondrial Dysfunction and Cancer Progression. Antioxidants 2018;7:13.
    doi: 10.3390/antiox7010013pmc: PMC5789323pubmed: 29337889google scholar: lookup
  59. Morte M.I., Rodrigues A.M., Soares D., Rodrigues A.S., Gamboa S., Ramalho-Santos J.. The Quantification of Lipid and Protein Oxidation in Stallion Spermatozoa and Seminal Plasma: Seasonal Distinctions and Correlations with DNA Strand Breaks, Classical Seminal Parameters and Stallion Fertility. Anim. Reprod. Sci. 2008;106:36–47.
    doi: 10.1016/j.anireprosci.2007.03.020pubmed: 17451892google scholar: lookup
  60. Agarwal A., Virk G., Ong C., Plessis S.S.D.. Effect of Oxidative Stress on Male Reproduction. World J. Mens Health 2014;32:1–17.
    doi: 10.5534/wjmh.2014.32.1.1pmc: PMC4026229pubmed: 24872947google scholar: lookup
  61. Katkov I.I., Mazur P.. Influence of Centrifugation Regimes on Motility, Yield, and Cell Associations of Mouse Spermatozoa. J. Androl. 1998;19:232–241.
    pubmed: 9570748

Citations

This article has been cited 8 times.
  1. Pehl S, Hundhammer T, Rimboeck J, Kraus R, Heckscher S, Kellermeier F, Gruber M, Wittmann S. Identification of the Centrifugation-Caused Paralytic Impact on Neutrophils. Cells 2025 Aug 30;14(17).
    doi: 10.3390/cells14171350pubmed: 40940760google scholar: lookup
  2. Lin HH, Mermillod P, Grasseau I, Blesbois E, Carvalho AV. Exploring how sucrose-colloid selection improves the fertilizing ability of chicken sperm after cryopreservation with glycerol. Poult Sci 2024 Mar;103(3):103448.
    doi: 10.1016/j.psj.2024.103448pubmed: 38237325google scholar: lookup
  3. Takalani NB, Monageng EM, Mohlala K, Monsees TK, Henkel R, Opuwari CS. Role of oxidative stress in male infertility. Reprod Fertil 2023 Jul 7;4(3).
    doi: 10.1530/RAF-23-0024pubmed: 37276172google scholar: lookup
  4. Hundhammer T, Gruber M, Wittmann S. Paralytic Impact of Centrifugation on Human Neutrophils. Biomedicines 2022 Nov 11;10(11).
    doi: 10.3390/biomedicines10112896pubmed: 36428463google scholar: lookup
  5. Pintus E, Ros-Santaella JL. Impact of Oxidative Stress on Male Reproduction in Domestic and Wild Animals. Antioxidants (Basel) 2021 Jul 20;10(7).
    doi: 10.3390/antiox10071154pubmed: 34356386google scholar: lookup
  6. Silvestre MA, Yániz JL, Peña FJ, Santolaria P, Castelló-Ruiz M. Role of Antioxidants in Cooled Liquid Storage of Mammal Spermatozoa. Antioxidants (Basel) 2021 Jul 8;10(7).
    doi: 10.3390/antiox10071096pubmed: 34356329google scholar: lookup
  7. Gimeno BF, Bariani MV, Laiz-Quiroga L, Martínez-León E, Von-Meyeren M, Rey O, Mutto AÁ, Osycka-Salut CE. Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen-Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status. Animals (Basel) 2021 Jan 3;11(1).
    doi: 10.3390/ani11010074pubmed: 33401609google scholar: lookup
  8. Sadeghi S, Del Gallego R, García-Colomer B, Gómez EA, Yániz JL, Gosálvez J, López-Fernández C, Silvestre MA. Effect of Sperm Concentration and Storage Temperature on Goat Spermatozoa during Liquid Storage. Biology (Basel) 2020 Sep 19;9(9).
    doi: 10.3390/biology9090300pubmed: 32961716google scholar: lookup
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