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Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen.
Abstract: The present study attempted to select the subpopulation of stallion spermatozoa that best survived a conventional freezing and thawing procedure, using centrifugation of post-thawed semen samples through a single layer of a glycidoxypropyltrimethoxysilane-coated silica colloid with a species-specific formulation (Androcoll-E). Sperm motility, sperm chromatin structure, membrane integrity and mitochondrial membrane potential were studied in filtered and non-filtered spermatozoa. Single-layer centrifugation (SLC) using Androcoll-E significantly improved all the sperm parameters studied, implying SLC may be a simple approach to improve the quality of frozen-thawed (FT) spermatozoa for AI.
Publication Date: 2008-08-29 PubMed ID: 18842364DOI: 10.1016/j.anireprosci.2008.08.025Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research explores the use of centrifugation on a single layer of colloid to selectively improve the quality of frozen-thawed sperm from stallions. Key semen characteristics like motility, chromatin structure, membrane integrity, and mitochondrial membrane potential showed significant improvement following this technique.
Objective of the Research
- The study was undertaken to find a subpopulation of stallion sperm that can withstand the freezing and thawing procedure commonly employed in Artificial Insemination (AI).
Methodology and Techniques Used
- Post-thaw semen samples went through centrifugation on a single layer of a silica colloid that had been coated with glycidoxypropyltrimethoxysilane. This colloid had a specific formulation for the species, known as Androcoll-E.
- The researchers focused on examining four key characteristics of the sperm: motility, chromatin structure, membrane integrity, and the potential of the mitochondrial membrane.
Findings of the Research
- Single Layer Centrifugation (SLC) using Androcoll-E brought about significant improvements in all the semen parameters under study.
- This suggests that SLC could be a straight forward approach to enhance the quality of frozen-thawed sperm used for AI.
Implications of the Research
- This study has potential implications for the field of equine reproduction, particularly for improving success rates in artificial insemination procedures.
- By enhancing the quality of frozen-thawed sperm, the likelihood of successful conception and healthy offspring can be increased.
Cite This Article
APA
Macías García B, Morrell JM, Ortega-Ferrusola C, González-Fernández L, Tapia JA, Rodriguez-Martínez H, Peña FJ.
(2008).
Centrifugation on a single layer of colloid selects improved quality spermatozoa from frozen-thawed stallion semen.
Anim Reprod Sci, 114(1-3), 193-202.
https://doi.org/10.1016/j.anireprosci.2008.08.025 Publication
Researcher Affiliations
- Veterinary Teaching Hospital, Laboratory of Spermatology, University of Extremadura, Avda de Universidad s/n, Cáceres, Spain.
MeSH Terms
- Animals
- Centrifugation / methods
- Colloids
- Cryopreservation / methods
- Cryopreservation / veterinary
- DNA Fragmentation
- Freezing
- Horses / physiology
- Male
- Semen Preservation / methods
- Semen Preservation / veterinary
- Sperm Motility
- Spermatozoa / cytology
- Spermatozoa / physiology
Citations
This article has been cited 12 times.- Al-Kass Z, Morrell JM, Ntallaris T. Effect of Centrifugation of Stallion Semen Through a Low Density Colloid Prior to Freezing on Sperm Cryosurvival. Animals (Basel) 2025 Jun 25;15(13).
- Al-Kass Z, Morrell JM. Freezing Stallion Semen-What Do We Need to Focus on for the Future?. Vet Sci 2024 Feb 2;11(2).
- Gutiérrez-Cepeda L, Crespo F, Blazquez JC, Serres C. Optimization of the Equine-Sperm Freeze Test in Purebred Spanish Horses by Incorporating Colloidal Centrifugation. Animals (Basel) 2023 Jan 22;13(3).
- Guillén Palomino CY, Fumuso FG, Bertuzzi ML, Giuliano SM, Velásquez González N, Bariani MV, Carretero MI. Use of Androcoll-E(TM) to Separate Frozen-Thawed Llama Sperm From Seminal Plasma and Diluent. Front Vet Sci 2020;7:594926.
- Gimeno BF, Bariani MV, Laiz-Quiroga L, Martínez-León E, Von-Meyeren M, Rey O, Mutto AÁ, Osycka-Salut CE. Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen-Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status. Animals (Basel) 2021 Jan 3;11(1).
- Ortiz I, Dorado J, Morrell JM, Diaz-Jimenez MA, Pereira B, Consuegra C, Hidalgo M. Comparison of sperm selection techniques in donkeys: motile subpopulations from a practical point of view. Anim Reprod 2019 Oct 23;16(2):282-289.
- Delgado-Bermúdez A, Noto F, Bonilla-Correal S, Garcia-Bonavila E, Catalán J, Papas M, Bonet S, Miró J, Yeste M. Cryotolerance of Stallion Spermatozoa Relies on Aquaglyceroporins rather than Orthodox Aquaporins. Biology (Basel) 2019 Nov 12;8(4).
- Gamboa S, Quaresma A, Castro F, Bravo P, Rebordão MR, Oom MDM, Rocha A. In vivo fertilizing ability of stallion spermatozoa processed by single layer centrifugation with Androcoll-E™. Saudi J Biol Sci 2017 Nov;24(7):1489-1496.
- Sjunnesson YC, Morrell JM, González R. Single layer centrifugation-selected boar spermatozoa are capable of fertilization in vitro. Acta Vet Scand 2013 Mar 5;55(1):20.
- Gutiérrez-Cepeda L, Fernández A, Crespo F, Ramírez MÁ, Gosálvez J, Serres C. The effect of two pre-cryopreservation single layer colloidal centrifugation protocols in combination with different freezing extenders on the fragmentation dynamics of thawed equine sperm DNA. Acta Vet Scand 2012 Dec 5;54(1):72.
- Gallardo Bolaños JM, Miró Morán Á, Balao da Silva CM, Morillo Rodríguez A, Plaza Dávila M, Aparicio IM, Tapia JA, Ortega Ferrusola C, Peña FJ. Autophagy and apoptosis have a role in the survival or death of stallion spermatozoa during conservation in refrigeration. PLoS One 2012;7(1):e30688.
- Morrell JM, Rodriguez-Martinez H. Practical applications of sperm selection techniques as a tool for improving reproductive efficiency. Vet Med Int 2010 Aug 4;2011.
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