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Home / Equine Research Bank / BMC Vet Res / Changes in biomarkers in equine synovial fluid two weeks aft...
BMC veterinary research2018; 14(1); 186; doi: 10.1186/s12917-018-1512-2

Changes in biomarkers in equine synovial fluid two weeks after intra-articular hyaluronan treatment: a randomised double-blind clinical trial.

Abstract: Inflammatory and degenerative activity inside the joint can be studied in vivo via analysis of synovial fluid (SF) biomarkers, which are molecular markers of inflammatory processes and tissue turnover. The aim of this study was to investigate the response of selected biomarkers in the SF after an intra-articular (IA) high-molecular-weight non-animal stabilized hyaluronic acid (NASHA) treatment. Our hypothesis was that prostaglandin E (PGE), substance P, aggrecan chondroitin sulfate 846 epitope (CS846), and carboxypeptide of type II collagen (CPII) concentrations in SF would decrease more in the NASHA than in the placebo group. Twenty-eight clinically lame horses with positive responses to diagnostic IA anaesthesia of the metacarpophalangeal or metatarsophalangeal joints were randomized into treatment (n = 15) and control (n = 13) groups. After collection of baseline SF samples followed by IA diagnostic anaesthesia, horses in the treatment group received 3 ml of a NASHA product IA. Those in the placebo group received an equivalent volume of sterile 0.9% saline solution. The horses were re-evaluated and a second SF sample was obtained after a 2-week period. Results: CS846 concentration decreased in the NASHA group only (P = 0.010). Both PGE and CPII concentrations decreased within the groups (PGE, P = 0.010 for the NASHA group; P = 0.027 for the placebo group; CPII, P < 0.001 for NASHA group; P = 0.009 for placebo group). No significant treatment effect for any biomarker was found between groups. NASHA induced an increase in white blood cell count; this was significant compared with baseline (P = 0.021) and the placebo group (P = 0.045). Conclusions: Although the SF concentration of the cartilage-derived biomarker CS846 decreased in the NASHA group, no statistically significant treatment effect of any of the biomarkers were observed between treatment groups. The significant increase in SF white blood cell count after IA NASHA may indicate a mild inflammatory response. However, as no clinical adverse effects were observed, we conclude that IA NASHA was well tolerated.
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Publication Date: 2018-06-15 PubMed ID: 29907111PubMed Central: PMC6003042DOI: 10.1186/s12917-018-1512-2Google Scholar: Lookup
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  • Journal Article
  • Randomized Controlled Trial

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the effects of a high-molecular-weight non-animal stabilized hyaluronic acid (NASHA) treatment on various biomarkers in horse synovial fluid. It tests the hypothesis that certain biomarker concentrations would decrease more in horses receiving NASHA treatment than in a placebo group. The results indicated no significant difference in changes of these biomarkers between the treatment and placebo group. However, the NASHA group showed higher white blood cell counts suggesting a mild inflammatory response.

Research Aim and Hypothesis

  • The purpose of this study was to explore the effects of a non-animal stabilized hyaluronic acid (NASHA) treatment in horses on selected synovial fluid (SF) biomarkers, which can indicate inflammation and tissue activity.
  • The researchers hypothesized that certain biomarker concentrations, specifically prostaglandin E, substance P, aggrecan chondroitin sulfate 846 epitope, and carboxypeptide of type II collagen would undergo steeper decrease in horses that received the NASHA treatment than in the placebo group.

Methodology

  • Twenty-eight horses with lameness and positive responses to diagnostic intra-articular anaesthesia were divided randomly into the treatment group (15 horses) and control group (13 horses).
  • Following collection of baseline SF samples, horses in the treatment group received a NASHA treatment while those in the placebo group were given a sterile saline solution.
  • After a two-week period, the horses were examined again and a second SF sample was obtained.

Results

  • The NASHA group recorded a decrease in concentration of CS846, one of the selected biomarkers.
  • Decreases in concentrations of both PGE and CPII biomarkers were observed within both the NASHA and placebo groups.
  • Overall, no significant difference was found between the treatment and placebo groups in terms of biomarker treatment effects.
  • However, the NASHA group exhibited an increase in white blood cell count, significant in comparison with the baseline and the placebo group. This may suggest a minor inflammatory response from the treatment.

Conclusions

  • The results showed a decrease in the SF concentration of the CS846 biomarker in the NASHA group.
  • However, no significant differences in the effects on any of the biomarkers were observed between the treatment and placebo groups.
  • The NASHA group did indicate a mild inflammatory reaction, as shown by the increase in white blood cells count.
  • This reaction did not lead to any clear adverse effects, leading to the conclusion that the NASHA treatment was well tolerated.

Cite This Article

APA
Niemelä TM, Tulamo RM, Aaltonen K, Sankari SM, Hielm-Björkman AK. (2018). Changes in biomarkers in equine synovial fluid two weeks after intra-articular hyaluronan treatment: a randomised double-blind clinical trial. BMC Vet Res, 14(1), 186. https://doi.org/10.1186/s12917-018-1512-2

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 14
Issue: 1
Pages: 186
PII: 186

Researcher Affiliations

Niemelä, Tytti M
  • Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland. tytti.niemela@fimnet.fi.
Tulamo, Riitta-Mari
  • Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland.
Aaltonen, Kaisa
  • Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland.
Sankari, Satu M
  • Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland.
Hielm-Björkman, Anna K
  • Department of Equine and Small Animal Medicine, Faculty of Veterinary Medicine, University of Helsinki, P.O. Box 57, 00014, Helsinki, Finland.

MeSH Terms

  • Animals
  • Biomarkers / metabolism
  • Cartilage, Articular
  • Female
  • Horse Diseases / drug therapy
  • Horse Diseases / metabolism
  • Horses
  • Hyaluronic Acid / therapeutic use
  • Lameness, Animal / drug therapy
  • Male
  • Synovial Fluid / metabolism
  • Synovitis / drug therapy
  • Synovitis / veterinary

Conflict of Interest Statement

ETHICS APPROVAL AND CONSENT TO PARTICIPATE: The study was approved by the Viikki Campus Research Ethics Committee of the University of Helsinki. All horse owners signed a study consent before the start of the study and owners were allowed withdraw from the study without giving any particular reason. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

References

This article includes 54 references
  1. Jeffcott LB, Rossdale PD, Freestone J, Frank CJ, Towers-Clark PF. An assessment of wastage in thoroughbred racing from conception to 4 years of age.. Equine Vet J 1982 Jul;14(3):185-98.
    doi: 10.1111/j.2042-3306.1982.tb02389.xpubmed: 7106081google scholar: lookup
  2. Morris EA, Seeherman HJ. Clinical evaluation of poor performance in the racehorse: the results of 275 evaluations.. Equine Vet J 1991 May;23(3):169-74.
    doi: 10.1111/j.2042-3306.1991.tb02749.xpubmed: 1884697google scholar: lookup
  3. Asheim A, Lindblad G. Intra-articular treatment of arthritis in race-horses with sodium hyaluronate.. Acta Vet Scand 1976;17(4):379-94.
    pmc: PMC8383961pubmed: 1015471doi: 10.1186/bf03547893google scholar: lookup
  4. Rose RJ. The intra-articular use of sodium hyaluronate for the treatment of osteo-arthrosis in the horse.. N Z Vet J 1979 Jan-Feb;27(1-2):5-8.
    doi: 10.1080/00480169.1979.34585pubmed: 286229google scholar: lookup
  5. Ruth DT, Swites BJ. Comparison of the effectiveness of intra-articular hyaluronic acid and conventional therapy for the treatment of naturally occurring arthritic conditions in horses.. Equine Pract 1985;7:25–29.
  6. Aviad AD, Arthur RM, Brencick VA. Synacid vs. Hylartin V in equine joint disease.. J Equine Vet Sci 1988;8:112–116.
    doi: 10.1016/S0737-0806(88)80030-3google scholar: lookup
  7. Gaustad G, Larsen S. Comparison of polysulphated glycosaminoglycan and sodium hyaluronate with placebo in treatment of traumatic arthritis in horses.. Equine Vet J 1995 Sep;27(5):356-62.
    doi: 10.1111/j.2042-3306.1995.tb04070.xpubmed: 8654350google scholar: lookup
  8. Gomis A, Pawlak M, Balazs EA, Schmidt RF, Belmonte C. Effects of different molecular weight elastoviscous hyaluronan solutions on articular nociceptive afferents.. Arthritis Rheum 2004 Jan;50(1):314-26.
    doi: 10.1002/art.11421pubmed: 14730630google scholar: lookup
  9. Caires R, Luis E, Taberner FJ, Fernandez-Ballester G, Ferrer-Montiel A, Balazs EA, Gomis A, Belmonte C, de la Peña E. Hyaluronan modulates TRPV1 channel opening, reducing peripheral nociceptor activity and pain.. Nat Commun 2015 Aug 27;6:8095.
    pmc: PMC4560824pubmed: 26311398doi: 10.1038/ncomms9095google scholar: lookup
  10. Punzi L, Schiavon F, Cavasin F, Ramonda R, Gambari PF, Todesco S. The influence of intra-articular hyaluronic acid on PGE2 and cAMP of synovial fluid.. Clin Exp Rheumatol 1989 May-Jun;7(3):247-50.
    pubmed: 2547540
  11. Frean SP, Lees P. Effects of polysulfated glycosaminoglycan and hyaluronan on prostaglandin E2 production by cultured equine synoviocytes.. Am J Vet Res 2000 May;61(5):499-505.
    doi: 10.2460/ajvr.2000.61.499pubmed: 10803643google scholar: lookup
  12. Frisbie DD, Kawcak CE, McIlwraith CW, Werpy NM. Evaluation of polysulfated glycosaminoglycan or sodium hyaluronan administered intra-articularly for treatment of horses with experimentally induced osteoarthritis.. Am J Vet Res 2009 Feb;70(2):203-9.
    doi: 10.2460/ajvr.70.2.203pubmed: 19231952google scholar: lookup
  13. Leopold SS, Warme WJ, Pettis PD, Shott S. Increased frequency of acute local reaction to intra-articular hylan GF-20 (synvisc) in patients receiving more than one course of treatment.. J Bone Joint Surg Am 2002 Sep;84(9):1619-23.
    doi: 10.2106/00004623-200209000-00015pubmed: 12208919google scholar: lookup
  14. Pullman-Mooar S, Mooar P, Sieck M, Clayburne G, Schumacher HR. Are there distinctive inflammatory flares after hylan g-f 20 intraarticular injections?. J Rheumatol 2002 Dec;29(12):2611-4.
    pubmed: 12465161
  15. Marino AA, Waddell DD, Kolomytkin OV, Pruett S, Sadasivan KK, Albright JA. Assessment of immunologic mechanisms for flare reactions to Synvisc.. Clin Orthop Relat Res 2006 Jan;442:187-94.
    doi: 10.1097/01.blo.0000185031.86478.a8pubmed: 16394759google scholar: lookup
  16. Nicholson AM, Trumble TN, Merritt KA, Brown MP. Associations of horse age, joint type, and osteochondral injury with serum and synovial fluid concentrations of type II collagen biomarkers in Thoroughbreds.. Am J Vet Res 2010 Jul;71(7):741-9.
    doi: 10.2460/ajvr.71.7.741pubmed: 20594075google scholar: lookup
  17. Frisbie DD, Al-Sobayil F, Billinghurst RC, Kawcak CE, McIlwraith CW. Changes in synovial fluid and serum biomarkers with exercise and early osteoarthritis in horses.. Osteoarthritis Cartilage 2008 Oct;16(10):1196-204.
    pubmed: 18442931doi: 10.1016/j.joca.2008.03.008google scholar: lookup
  18. Bertone AL, Palmer JL, Jones J. Synovial fluid cytokines and eicosanoids as markers of joint disease in horses.. Vet Surg 2001 Nov-Dec;30(6):528-38.
    doi: 10.1053/jvet.2001.28430pubmed: 11704948google scholar: lookup
  19. Gibson KT, Hodge H, Whittem T. Inflammatory mediators in equine synovial fluid.. Aust Vet J 1996 Apr;73(4):148-51.
    doi: 10.1111/j.1751-0813.1996.tb10008.xpubmed: 8660230google scholar: lookup
  20. Todhunter PG, Kincaid SA, Todhunter RJ, Kammermann JR, Johnstone B, Baird AN, Hanson RR, Wright JM, Lin HC, Purohit RC. Immunohistochemical analysis of an equine model of synovitis-induced arthritis.. Am J Vet Res 1996 Jul;57(7):1080-93.
    pubmed: 8807026
  21. Kirker-Head CA, Chandna VK, Agarwal RK, Morris EA, Tidwell A, O'Callaghan MW, Rand W, Kumar MS. Concentrations of substance P and prostaglandin E2 in synovial fluid of normal and abnormal joints of horses.. Am J Vet Res 2000 Jun;61(6):714-8.
    doi: 10.2460/ajvr.2000.61.714pubmed: 10850851google scholar: lookup
  22. Frisbie DD, McIlwraith CW, Kawcak CE, Werpy NM. Evaluation of intra-articular hyaluronan, sodium chondroitin sulfate and N-acetyl-D-glucosamine combination versus saline (0.9% NaCl) for osteoarthritis using an equine model.. Vet J 2013 Sep;197(3):824-9.
    doi: 10.1016/j.tvjl.2013.05.033pubmed: 23838209google scholar: lookup
  23. van Loon JP, de Grauw JC, van Dierendonck M, L'ami JJ, Back W, van Weeren PR. Intra-articular opioid analgesia is effective in reducing pain and inflammation in an equine LPS induced synovitis model.. Equine Vet J 2010 Jul;42(5):412-9.
    doi: 10.1111/j.2042-3306.2010.00077.xpubmed: 20636777google scholar: lookup
  24. Piat P, Richard H, Beauchamp G, Laverty S. In vivo effects of a single intra-articular injection of 2% lidocaine or 0.5% bupivacaine on articular cartilage of normal horses.. Vet Surg 2012 Nov;41(8):1002-10.
    doi: 10.1111/j.1532-950X.2012.01039.xpubmed: 22957715google scholar: lookup
  25. Robion FC, Doizé B, Bouré L, Marcoux M, Ionescu M, Reiner A, Poole AR, Laverty S. Use of synovial fluid markers of cartilage synthesis and turnover to study effects of repeated intra-articular administration of methylprednisolone acetate on articular cartilage in vivo.. J Orthop Res 2001 Mar;19(2):250-8.
    doi: 10.1016/S0736-0266(00)90008-1pubmed: 11347698google scholar: lookup
  26. Moraes AP, Moreira JJ, Brossi PM, Machado TS, Michelacci YM, Baccarin RY. Short- and long-term effects of platelet-rich plasma upon healthy equine joints: Clinical and laboratory aspects.. Can Vet J 2015 Aug;56(8):831-8.
    pmc: PMC4502851pubmed: 26246629
  27. Schleining JA, McClure SR, Evans RB, Hyde WG, Wulf LW, Kind AJ. Liposome-based diclofenac for the treatment of inflammation in an acute synovitis model in horses.. J Vet Pharmacol Ther 2008 Dec;31(6):554-61.
    doi: 10.1111/j.1365-2885.2008.00994.xpubmed: 19000279google scholar: lookup
  28. de Grauw JC, van de Lest CH, Brama PA, Rambags BP, van Weeren PR. In vivo effects of meloxicam on inflammatory mediators, MMP activity and cartilage biomarkers in equine joints with acute synovitis.. Equine Vet J 2009 Sep;41(7):693-9.
    doi: 10.2746/042516409X436286pubmed: 19927589google scholar: lookup
  29. Owens JG, Kamerling SG, Stanton SR, Keowen ML, Prescott-Mathews JS. Effects of pretreatment with ketoprofen and phenylbutazone on experimentally induced synovitis in horses.. Am J Vet Res 1996 Jun;57(6):866-74.
    pubmed: 8725815
  30. Marshall KW, Chiu B, Inman RD. Substance P and arthritis: analysis of plasma and synovial fluid levels.. Arthritis Rheum 1990 Jan;33(1):87-90.
    doi: 10.1002/art.1780330111pubmed: 1689161google scholar: lookup
  31. de Grauw JC, van de Lest CH, van Weeren R, Brommer H, Brama PA. Arthrogenic lameness of the fetlock: synovial fluid markers of inflammation and cartilage turnover in relation to clinical joint pain.. Equine Vet J 2006 Jul;38(4):305-11.
    doi: 10.2746/042516406777749236pubmed: 16866196google scholar: lookup
  32. Niemelä TM, Tulamo RM, Hielm-Björkman AK. A randomised, double-blinded, placebo-controlled clinical study on intra-articular hyaluronan treatment in equine lameness originating from the metacarpophalangeal joint.. BMC Vet Res 2016 Mar 23;12:60.
    doi: 10.1186/s12917-016-0687-7pmc: PMC4804525pubmed: 27005478google scholar: lookup
  33. Brown TJ, Laurent UB, Fraser JR. Turnover of hyaluronan in synovial joints: elimination of labelled hyaluronan from the knee joint of the rabbit.. Exp Physiol 1991 Jan;76(1):125-34.
    doi: 10.1113/expphysiol.1991.sp003474pubmed: 2015069google scholar: lookup
  34. Lindenhayn K, Heilmann HH, Niederhausen T, Walther HU, Pohlenz K. Elimination of tritium-labelled hyaluronic acid from normal and osteoarthritic rabbit knee joints.. Eur J Clin Chem Clin Biochem 1997 May;35(5):355-63.
    pubmed: 9189739doi: 10.1515/cclm.1997.35.5.355google scholar: lookup
  35. Edsman K, Hjelm R, Lärkner H, Nord LI, Karlsson A, Wiebensjö Å, Höglund AU, Kenne AH, Näsström J. Intra-articular Duration of Durolane™ after Single Injection into the Rabbit Knee.. Cartilage 2011 Oct;2(4):384-8.
    doi: 10.1177/1947603511400184pmc: PMC4297137pubmed: 26069596google scholar: lookup
  36. Dougados M, Nguyen M, Listrat V, Amor B. High molecular weight sodium hyaluronate (hyalectin) in osteoarthritis of the knee: a 1 year placebo-controlled trial.. Osteoarthritis Cartilage 1993 Apr;1(2):97-103.
    doi: 10.1016/S1063-4584(05)80024-Xpubmed: 8886085google scholar: lookup
  37. Åkermark C, Berg P, Björkman A, Malm P. Non-animal stabilised hyaluronic acid in the treatment of osteoarthritis of the knee: a tolerability study.. Clin Drug Investig 2002;22:157–166.
    doi: 10.2165/00044011-200222030-00002google scholar: lookup
  38. Lindqvist U, Tolmachev V, Kairemo K, Aström G, Jonsson E, Lundqvist H. Elimination of stabilised hyaluronan from the knee joint in healthy men.. Clin Pharmacokinet 2002;41(8):603-13.
    doi: 10.2165/00003088-200241080-00004pubmed: 12102643google scholar: lookup
  39. Altman RD, Akermark C, Beaulieu AD, Schnitzer T. Efficacy and safety of a single intra-articular injection of non-animal stabilized hyaluronic acid (NASHA) in patients with osteoarthritis of the knee.. Osteoarthritis Cartilage 2004 Aug;12(8):642-9.
    doi: 10.1016/j.joca.2004.04.010pubmed: 15262244google scholar: lookup
  40. Working in Epidemiology. 2010. http://www.winepi.net/uk/index.htm. Accessed 1 Feb 2010.
  41. Richette P, Ravaud P, Conrozier T, Euller-Ziegler L, Mazières B, Maugars Y, Mulleman D, Clerson P, Chevalier X. Effect of hyaluronic acid in symptomatic hip osteoarthritis: a multicenter, randomized, placebo-controlled trial.. Arthritis Rheum 2009 Mar;60(3):824-30.
    doi: 10.1002/art.24301pubmed: 19248105google scholar: lookup
  42. Pham T, Le Henanff A, Ravaud P, Dieppe P, Paolozzi L, Dougados M. Evaluation of the symptomatic and structural efficacy of a new hyaluronic acid compound, NRD101, in comparison with diacerein and placebo in a 1 year randomised controlled study in symptomatic knee osteoarthritis.. Ann Rheum Dis 2004 Dec;63(12):1611-7.
    doi: 10.1136/ard.2003.019703pmc: PMC1754857pubmed: 15331394google scholar: lookup
  43. Misheff MM, Stover SM. A comparison of two techniques for arthrocentesis of the equine metacarpophalangeal joint.. Equine Vet J 1991 Jul;23(4):273-6.
    doi: 10.1111/j.2042-3306.1991.tb03716.xpubmed: 1915226google scholar: lookup
  44. Kwan C, Bell R, Koenig T, Bischofberger A, Horadagoda N, Perkins NR, Jeffcott LB, Dart AJ. Effects of intra-articular sodium pentosan polysulfate and glucosamine on the cytology, total protein concentration and viscosity of synovial fluid in horses.. Aust Vet J 2012 Aug;90(8):315-20.
    doi: 10.1111/j.1751-0813.2012.00959.xpubmed: 22827626google scholar: lookup
  45. Sanchez Teran AF, Rubio-Martinez LM, Villarino NF, Sanz MG. Effects of repeated intra-articular administration of amikacin on serum amyloid A, total protein and nucleated cell count in synovial fluid from healthy horses.. Equine Vet J Suppl 2012 Dec;(43):12-6.
    doi: 10.1111/j.2042-3306.2012.00637.xpubmed: 23447871google scholar: lookup
  46. Stover SM, Pool RR. Effect of intra-articular gentamicin sulfate on normal equine synovial membrane.. Am J Vet Res 1985 Dec;46(12):2485-91.
    pubmed: 4083580
  47. Caron JP. Osteoarthritis.. In: Ross MW, Dyson SJ, editors. Lameness in the Horse. 2. St Louis: Saunders; 2011. pp. 655–668.
  48. Carmona JU, Argüelles D, Deulofeu R, Martínez-Puig D, Prades M. Effect of the administration of an oral hyaluronan formulation on clinical and biochemical parameters in young horses with osteochondrosis.. Vet Comp Orthop Traumatol 2009;22(6):455-9.
    doi: 10.3415/VCOT-09-01-0001pubmed: 19876524google scholar: lookup
  49. Frisbie DD, McIlwraith CW, Kawcak CE, Werpy NM, Pearce GL. Evaluation of topically administered diclofenac liposomal cream for treatment of horses with experimentally induced osteoarthritis.. Am J Vet Res 2009 Feb;70(2):210-5.
    doi: 10.2460/ajvr.70.2.210pubmed: 19231953google scholar: lookup
  50. Lohmander LS, Ionescu M, Jugessur H, Poole AR. Changes in joint cartilage aggrecan after knee injury and in osteoarthritis.. Arthritis Rheum 1999 Mar;42(3):534-44.
    doi: 10.1002/1529-0131(199904)42:3<534::AID-ANR19>3.0.CO;2-Jpubmed: 10088777google scholar: lookup
  51. Koenig TJ, Dart AJ, McIlwraith CW, Horadagoda N, Bell RJ, Perkins N, Dart C, Krockenberger M, Jeffcott LB, Little CB. Treatment of experimentally induced osteoarthritis in horses using an intravenous combination of sodium pentosan polysulfate, N-acetyl glucosamine, and sodium hyaluronan.. Vet Surg 2014 Jul;43(5):612-22.
    doi: 10.1111/j.1532-950X.2014.12203.xpubmed: 24819506google scholar: lookup
  52. de Grauw JC, van Loon JP, van de Lest CH, Brunott A, van Weeren PR. In vivo effects of phenylbutazone on inflammation and cartilage-derived biomarkers in equine joints with acute synovitis.. Vet J 2014 Jul;201(1):51-6.
    doi: 10.1016/j.tvjl.2014.03.030pubmed: 24888681google scholar: lookup
  53. de Grauw JC, van de Lest CH, van Weeren PR. Inflammatory mediators and cartilage biomarkers in synovial fluid after a single inflammatory insult: a longitudinal experimental study.. Arthritis Res Ther 2009;11(2):R35.
    doi: 10.1186/ar2640pmc: PMC2688180pubmed: 19272138google scholar: lookup
  54. Johnson KA, Hay CW, Chu Q, Roe SC, Caterson B. Cartilage-derived biomarkers of osteoarthritis in synovial fluid of dogs with naturally acquired rupture of the cranial cruciate ligament.. Am J Vet Res 2002 Jun;63(6):775-81.
    doi: 10.2460/ajvr.2002.63.775pubmed: 12061519google scholar: lookup

Citations

This article has been cited 1 times.
  1. Niemelä TM, Tulamo RM, Carmona JU, López C. Evaluation of the effect of experimentally induced cartilage defect and intra-articular hyaluronan on synovial fluid biomarkers in intercarpal joints of horses.. Acta Vet Scand 2019 May 30;61(1):24.
    doi: 10.1186/s13028-019-0460-6pubmed: 31146775google scholar: lookup
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