Changes in equine endometrial retinol-binding protein RNA during the estrous cycle and early pregnancy and with exogenous steroids.
Abstract: A cDNA library was constructed from poly(A) RNA obtained from Day 14 nonbred equine endometrium. A cDNA probe for porcine retinol-binding protein (RBP) was used to screen the library, and a complete cDNA sequence (1133 bp, excluding the poly(A) tail) was obtained. Endometrial biopsies were obtained from cycling, nonbred mares at Days 0, 1, 4, 8, 10, 11, 13, and 15 and from pregnant mares at Days 11, 13, 15, and 17 after ovulation (n = 2 mares each day). Endometrial biopsies were also taken from 18 noncycling anestrous mares after the following treatments: C (vehicle control for 1 day, n = 3), E (estradiol-17 valerate, 5 mg/day for 6 days, n = 3), P (progesterone, 250 mg/day for 6 days, n = 4), ESP (E for 6 days followed by, P for 6 days, n = 4), and ELP (E for 6 days followed by P for 12 days, n = 4). Northern blot analyses were performed on total RNA (30 micrograms) using cDNA probes to equine (e) RBP and human glyceraldehyde-3 phosphate dehydrogenase (G3PDH). The RBP RNA levels (normalized to G3PDH) from nonbred mares were low during early diestrus and increased after Day 10, and RBP RNA levels from pregnant mares were similar to those of nonbred mares for corresponding days. E tended to decrease endometrial RBP RNA; and P, ESP, and ELP increased it compared to C. There were no significant differences among P, ESP, and ELP RBP RNA levels.(ABSTRACT TRUNCATED AT 250 WORDS)
Publication Date: 1995-02-01 PubMed ID: 7536053DOI: 10.1095/biolreprod52.2.438Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research studies changes in equine endometrial retinol-binding protein (RBP) RNA during different periods of a horse’s reproduction cycle and early pregnancy, as well as with the introduction of external steroids.
Research Methodology
- The researchers constructed a cDNA library using poly(A) RNA obtained from Day 14 nonbred equine endometrium.
- They used a cDNA probe for porcine retinol-binding protein (RBP) to screen this library, and extracted a full cDNA sequence.
- Endometrial biopsies were collected from cycling, nonbred mares on various days of the estrous cycle and from pregnant mares at specific days after ovulation.
- Same procedures were followed with noncycling, anestrous mares, following different hormonal treatments. These included treatment with control vehicle (C), estradiol-17 valerate (E), progesterone (P), estradiol + progesterone (ESP), and extended estradiol + progesterone (ELP).
- Northern blot analyses were performed on total RNA using cDNA probes to equine retinol-binding protein and human glyceraldehyde-3 phosphate dehydrogenase (G3PDH).
Research Findings
- RBP RNA levels from nonbred mares were low during early diestrus and increased after Day 10.
- RBP RNA levels from pregnant mares were similar to those of nonbred mares for corresponding days, indicating consistency between nonbred and pregnant mares at specific periods of their reproductive cycle.
- Research findings showed estradiol treatment tended to decrease the endometrial RBP RNA level while progesterone and its combinations (ESP and ELP) increased it when compared to the control group. However, there were no significant differences among RBP RNA levels in the progesterone, ESP, and ELP treated groups.
This research offers valuable insight into the impact of various hormones and states of the reproductive cycle on retinol-binding protein RNA in horses, which contributes to the understanding of equine reproductive biology.
Cite This Article
APA
McDowell KJ, Adams MH, Franklin KM, Baker CB.
(1995).
Changes in equine endometrial retinol-binding protein RNA during the estrous cycle and early pregnancy and with exogenous steroids.
Biol Reprod, 52(2), 438-443.
https://doi.org/10.1095/biolreprod52.2.438 Publication
Researcher Affiliations
- Gluck Equine Research Center, Department of Veterinary Science, University of Kentucky, Lexington 40546-0099, USA.
MeSH Terms
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA, Complementary / chemistry
- Endometrium / drug effects
- Endometrium / metabolism
- Estradiol / administration & dosage
- Estradiol / analogs & derivatives
- Estradiol / pharmacology
- Estrus / metabolism
- Female
- Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
- Horses / metabolism
- Humans
- Molecular Sequence Data
- Pregnancy
- Pregnancy, Animal / metabolism
- Progesterone / administration & dosage
- Progesterone / pharmacology
- RNA / metabolism
- Retinol-Binding Proteins / chemistry
- Retinol-Binding Proteins / genetics
- Retinol-Binding Proteins / metabolism
Citations
This article has been cited 3 times.- Aurich C, Budik S. Early pregnancy in the horse revisited - does exception prove the rule?. J Anim Sci Biotechnol 2015;6:50.
- Jessen KA, Satre MA. Mouse retinol binding protein gene: cloning, expression and regulation by retinoic acid. Mol Cell Biochem 2000 Aug;211(1-2):85-94.
- Crossett B, Allen WR, Stewart F. A 19 kDa protein secreted by the endometrium of the mare is a novel member of the lipocalin family. Biochem J 1996 Nov 15;320 ( Pt 1)(Pt 1):137-43.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
