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Animals : an open access journal from MDPI2022; 12(9); doi: 10.3390/ani12091169

Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach.

Abstract: Changes in the salivary proteome in 12 horses with the two diseases included in equine gastric ulcer syndrome (EGUS), equine glandular gastric disease (EGGD) (n = 6) and equine squamous gastric disease (ESGD) (n = 6), were evaluated using a high-resolution LC-MS/MS analysis of TMT-labelled peptides and compared to 10 healthy control horses. Serum was also analysed for comparative purposes. The comparison between the horses with EGGD and controls showed significant changes in 10 salivary proteins, whereas 36 salivary proteins were differently abundant between ESGD and control groups. The most upregulated proteins in the case of EGGD were related to immune activation whereas, in horses with ESGD, the most significantly changed proteins were associated with squamous cell regulation and growth. Compared to serum, saliva showed a higher number of proteins with significant changes and a different pattern of changes. The proteins identified in our study, in addition to providing new information about the pathophysiological mechanisms in these diseases, could have the potential to be novel biomarkers for the diagnosis or monitoring of EGGD and ESGD.
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Publication Date: 2022-05-02 PubMed ID: 35565595PubMed Central: PMC9103582DOI: 10.3390/ani12091169Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates changes in the composition of proteins in the saliva and serum of horses affected by equine gastric ulcer syndrome, specifically looking at two diseases. These protein changes are measured and compared with healthy horses, with the goal of identifying potential new biomarkers for disease diagnosis and monitoring.

Research Methods

  • The researchers studied changes in the saliva’s proteome (the entire set of proteins expressed by a genome, cell, tissue, or organism) in 12 horses diagnosed with two diseases categorized under equine gastric ulcer syndrome (EGUS): equine glandular gastric disease (EGGD) and equine squamous gastric disease (ESGD).
  • A detailed analysis of TMT-labelled peptides was carried out using high-resolution LC-MS/MS, a highly sensitive and advanced proteomic technique. This method was used to compare the salivary proteome of affected horses with that of 10 healthy control horses.
  • The same comparative analysis was also carried out on serum samples from these horses.

Major Findings

  • In the comparison between horses affected with EGGD and the controls, the researchers found significant changes in 10 salivary proteins. Meanwhile, 36 salivary proteins were found to be differently abundant between horses with ESGD and the control group.
  • In EGGD-affected horses, the proteins that showed the most increase were related to immune activation. Conversely, in horses with ESGD, the most notably changed proteins were associated with regulation and growth of squamous cells (a type of skin cell).
  • Compared to serum, saliva showed a higher number of proteins with significant changes, and these changes exhibited a different pattern.

Implications of the Research

  • The changes in salivary and serum proteins provide new insights into the pathophysiological mechanisms of EGGD and ESGD. This knowledge can help in developing targeted therapies and management strategies for these diseases.
  • The identified proteins have the potential to act as novel biomarkers for the diagnosis or monitoring of EGGD and ESGD. This could lead to quicker and more accurate diagnoses, better disease management, and improved prognostic information.

Cite This Article

APA
Muñoz-Prieto A, Contreras-Aguilar MD, Cerón JJ, Ayala I, Martin-Cuervo M, Gonzalez-Sanchez JC, Jacobsen S, Kuleš J, Beletić A, Rubić I, Mrljak V, Tecles F, Hansen S. (2022). Changes in Proteins in Saliva and Serum in Equine Gastric Ulcer Syndrome Using a Proteomic Approach. Animals (Basel), 12(9). https://doi.org/10.3390/ani12091169

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 12
Issue: 9

Researcher Affiliations

Muñoz-Prieto, Alberto
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
Contreras-Aguilar, Maria Dolores
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
Cerón, Jose Joaquín
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
Ayala, Ignacio
  • Department of Animal Medicine & Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
Martin-Cuervo, Maria
  • Medicine Animal, Faculty of Veterinary Medicine of Cáceres, University of Extremadura, Avenida de la Universidad S-N, 10002 Cáceres, Spain.
Gonzalez-Sanchez, Juan Carlos
  • BioQuant, Faculty of Biosciences, Heidelberg University, Im Neuenheimer Feld 267, 69117 Heidelberg, Germany.
Jacobsen, Stine
  • Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark.
Kuleš, Josipa
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
Beletić, Anđelo
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
Rubić, Ivana
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
Mrljak, Vladimir
  • Clinic for Internal Diseases, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10000 Zagreb, Croatia.
Tecles, Fernando
  • Interdisciplinary Laboratory of Clinical Analysis of the University of Murcia (Interlab-UMU), Department of Animal Medicine and Surgery, Veterinary School, Regional Campus of International Excellence Mare Nostrum, Campus de Espinardo, University of Murcia, 30100 Murcia, Spain.
Hansen, Sanni
  • Department of Veterinary Clinical Sciences, Section Medicine and Surgery, University of Copenhagen, Agrovej 8, 2630 Taastrup, Denmark.

Grant Funding

  • 19894/GERM/15 / Fundación Seneca
  • KK.01.1.16.0004 / European Structural and Investment Funds

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; the collection, analyses, or interpretation of data; the writing of the manuscript; nor in the decision to publish the results.

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Citations

This article has been cited 11 times.
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