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Home / Equine Research Bank / BMC Vet Res / Changes in saliva analytes in equine acute abdominal disease...
BMC veterinary research2019; 15(1); 187; doi: 10.1186/s12917-019-1933-6

Changes in saliva analytes in equine acute abdominal disease: a sialochemistry approach.

Abstract: The biochemical components of saliva can change in certain pathologies in horses, for example in acute abdominal disease. The aim of this study was (1) to evaluate if a panel of biochemical analytes usually used in serum can be measured in saliva of horses and (2) to study the possible changes of these biochemical analytes in saliva of horses affected by acute abdominal disease. A panel of 23 analytes was analytically validated in saliva of horses and possible changes in these analytes in a pilot study with six healthy horses and six horses with acute abdominal disease were evaluated. The analytes with significant changes were then evaluated in a larger population of 20 healthy and 37 diseased horses. Results: Seven analytes showed significant increases in the pilot study which were confirmed in the larger population. The analytes which showed significant changes, and their median fold increase and significance shown in the larger population were salivary γ-glutamyl transferase (gGT, 2.3 fold, P = 0.001), creatine kinase (CK, 6.2 fold, P < 0.001), urea (2.3 fold, P = 0.001), total bilirubin (2.6 fold, P < 0.001), total proteins (3.2 fold, P < 0.001), phosphorus (P, 4.5 fold, P < 0.001) and alpha-amylase (sAA, 8.5 fold, P < 0.001). Total proteins, P and sAA showed sensitivities higher than 70% at their optimal cut-off points and a specificity of 100% in differentiating between healthy horses and those with acute abdominal disease. Conclusions: A panel of 23 biochemical analytes can be measured in saliva of horses, where gGT, CK, urea, total bilirubin, total protein, P and sAA levels are raised in horses with acute abdominal disease.
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Publication Date: 2019-06-06 PubMed ID: 31170977PubMed Central: PMC6554884DOI: 10.1186/s12917-019-1933-6Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a study exploring changes in salivary biochemical analytes in horses with acute abdominal disease, validating that a panel of 23 specific biochemicals could be measured in horses’ saliva, some of which showed significant elevations in horses with this disease.

Objective of the Research

  • The research aimed at exploring the biochemical components found in horses’ saliva and understanding how these components might change in instances of acute abdominal disease.
  • Specifically, the study intended to validate whether a chosen panel of 23 biochemical analytes, usually tested in serum, could also be measured in horse saliva.
  • Another goal was to explore if the levels of these analytes changed in horses suffering from acute abdominal disease, indicating their potential as diagnostic markers.

Pilot Study and Results

  • The researchers started with a pilot study involving six healthy horses and six horses with acute abdominal disease, examining potential changes in the 23 selected biochemical analytes in the saliva.
  • The pilot study identified seven of the 23 analytes showing significant increases in the horses with abdominal disease compared to the healthy horses.

Main Study and Findings

  • The analytes with significant changes in the pilot study were then further examined in a larger population of 57 horses (20 healthy and 37 with abdominal disease).
  • The analytes found to significantly increase in the diseased horses were: salivary γ-glutamyl transferase (gGT), creatine kinase (CK), urea, total bilirubin, total proteins, phosphorus (P), and alpha-amylase (sAA).
  • In terms of sensitivity and specificity for detecting acute abdominal disease in horses, total proteins, P, and sAA yielded sensitivities greater than 70% at their optimal cut-off points and achieved a specificity of 100%.

Conclusions

  • The study’s findings validate that the 23 selected biochemical analytes can indeed be measured in horse saliva.
  • Moreover, the study found that the levels of gGT, CK, urea, total bilirubin, total proteins, P, and sAA significantly increase in horses suffering from acute abdominal disease.
  • In particular, total proteins, P, and sAA show high potential as reliable markers for the diagnosis of acute abdominal disease in horses due to their high sensitivity and specificity.

Cite This Article

APA
Contreras-Aguilar MD, Escribano D, Martínez-Subiela S, Martín-Cuervo M, Lamy E, Tecles F, Cerón JJ. (2019). Changes in saliva analytes in equine acute abdominal disease: a sialochemistry approach. BMC Vet Res, 15(1), 187. https://doi.org/10.1186/s12917-019-1933-6

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 15
Issue: 1
Pages: 187
PII: 187

Researcher Affiliations

Contreras-Aguilar, María Dolores
  • Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Campus de Espinardo s/n, 30100, Espinardo, Murcia, Spain.
Escribano, Damián
  • Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Campus de Espinardo s/n, 30100, Espinardo, Murcia, Spain.
  • Department of Food and Animal Science, School of Veterinary Medicine, University of Barcelona, Bellaterra, 08193, Barcelona, Spain.
Martínez-Subiela, Silvia
  • Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Campus de Espinardo s/n, 30100, Espinardo, Murcia, Spain.
Martín-Cuervo, María
  • Animal Medicine, Faculty of Veterinary Medicine of Cáceres, University of Extremadura, Av. de la Universidad S-N, 10005, Cáceres, Spain.
Lamy, Elsa
  • Institute of Mediterranean Agricultural and Environmental Sciences, University of Évora, Núcleo da Mitra, Apartado 94, 7006-554, Évora, Portugal.
Tecles, Fernando
  • Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Campus de Espinardo s/n, 30100, Espinardo, Murcia, Spain. ftecles@um.es.
Cerón, Jose Joaquín
  • Interdisciplinary Laboratory of Clinical Analysis (Interlab-UMU), Veterinary School, Campus of Excellence Mare Nostrum, University of Murcia, Campus de Espinardo s/n, 30100, Espinardo, Murcia, Spain.

MeSH Terms

  • Abdomen, Acute / diagnosis
  • Abdomen, Acute / veterinary
  • Animals
  • Bilirubin / analysis
  • Female
  • Gastrointestinal Diseases / diagnosis
  • Gastrointestinal Diseases / veterinary
  • Horse Diseases / diagnosis
  • Horses
  • Male
  • Phosphorus / analysis
  • Saliva / chemistry
  • Saliva / enzymology
  • Salivary Proteins and Peptides / analysis
  • Sensitivity and Specificity
  • Urea / analysis

Grant Funding

  • 19894/GERM/15 / Fundaciu00f3n Su00e9neca
  • R- 605/2016 / Universidad de Murcia
  • IF/01778/2013 / Portuguese Science Foundation

Conflict of Interest Statement

None of the authors has any financial or personal relationships that could inappropriately influence or bias the content of the paper. Silvia Martínez-Subiela, Elsa Lamy and Jose Joaquín Cerón are editorial board members of BMC Veterinary Research.

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Citations

This article has been cited 13 times.
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