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Home/Equine Research Bank/Vet J/Evaluation of a commercially available human serum amyloid A...
Veterinary journal (London, England : 1997)2005; 172(2); 315-319; doi: 10.1016/j.tvjl.2005.04.021

Evaluation of a commercially available human serum amyloid A (SAA) turbidometric immunoassay for determination of equine SAA concentrations.

Abstract: The aim of the present study was to evaluate whether equine serum amyloid A (SAA) concentrations could be measured reliably with a turbidometric immunoassay (TIA) developed for use with human serum. Intra- and inter-assay imprecision were evaluated by multiple measurements on equine serum pools. Assay inaccuracy was determined by linearity under dilution. The assay was subsequently used for measuring SAA concentrations in clinically healthy horses, horses with inflammatory diseases, horses with non-inflammatory diseases, and in horses before and after castration. In pools with low, intermediate and high SAA concentrations, the intra-assay imprecisions were 24.4%, 1.6% and 2.1%, and the inter-assay imprecisions were 33.2%, 4.6% and 6.5%. Slight signs of inaccuracy were observed, but these inaccuracies were negligible when considering the large dynamic range of the SAA response. The assay was able to detect the expected difference in SAA levels in different groups of horses. It was also able to demonstrate the expected dynamic changes in SAA after castration. In conclusion, equine SAA concentrations can be measured reliably using the TIA designed for human SAA.
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Publication Date: 2005-06-13 PubMed ID: 15950503DOI: 10.1016/j.tvjl.2005.04.021Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article evaluates the use of a human serum amyloid A (SAA) turbidometric immunoassay to reliably measure equine SAA concentrations. The study validates its effectiveness in measuring SAA levels in various groups of horses and determining such concentrations before and after equine castration.

Objective and Methodology of the Study

  • The aim of this research was to assess the ability of a turbidometric immunoassay (TIA) initially developed for human SAA in accurately determining equine (horse) SAA concentrations.
  • The study evaluated both intra-assay (within the same assay) and inter-assay (between different assays) imprecision by conducting multiple measurements on equine serum pools.
  • Assay inaccuracy was determined through linearity under dilution, a process that involves diluting the assay and then checking its linear response.
  • The TIA was then utilized to measure SAA concentrations in several categories of horses: clinically healthy horses, those with inflammatory diseases, ones with non-inflammatory diseases, and horses pre and post castration.

Study Findings

  • The study found varying degrees of intra-assay imprecisions (24.4%, 1.6%, and 2.1%) and inter-assay imprecisions (33.2%, 4.6%, and 6.5%) for low, intermediate, and high SAA concentrations respectively.
  • Minor signs of inaccuracy were observed, but they proved insignificant considering the broad dynamic range of the SAA response.
  • The TIA effectively detected the expected difference in SAA levels across the different groups of horses being studied.
  • Additionally, the TIA successfully highlighted changes in SAA levels before and after the castration of horses – an expected dynamic change.

Conclusion

  • The study concluded positively and affirmed that equine SAA concentrations can be measured reliably using the TIA designed initially for human SAA.
  • This finding provides a new tool for veterinarians and researchers to assess SAA levels in horses, which can aid in the diagnosis and monitoring of various equine diseases.

Cite This Article

APA
Jacobsen S, Kjelgaard-Hansen M, Hagbard Petersen H, Jensen AL. (2005). Evaluation of a commercially available human serum amyloid A (SAA) turbidometric immunoassay for determination of equine SAA concentrations. Vet J, 172(2), 315-319. https://doi.org/10.1016/j.tvjl.2005.04.021

Publication

Veterinary journal (London, England : 1997)
ISSN: 1090-0233
NlmUniqueID: 9706281
Country: England
Language: English
Volume: 172
Issue: 2
Pages: 315-319

Researcher Affiliations

Jacobsen, S
  • Department of Large Animal Sciences, The Royal Veterinary and Agricultural University, Dyrlagevej 48, 1870 Frederiksberg C, Copenhagen, Denmark. stj@kvl.dk
Kjelgaard-Hansen, M
    Hagbard Petersen, H
      Jensen, A L

        MeSH Terms

        • Animals
        • Horse Diseases / blood
        • Horses / blood
        • Immunoassay / methods
        • Immunoassay / veterinary
        • Nephelometry and Turbidimetry / veterinary
        • Reproducibility of Results
        • Serum Amyloid A Protein / analysis

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