Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61).
Abstract: This paper describes two anti-glycoprotein IIb/IIIa or CD41/61 murine monoclonal antibodies (Co.35E4 and Co.2oA1). The cellular distribution and apparent molecular weight of the antigen detected by these antibodies is consistent with their reaction with ruminant and equine glycoprotein IIb/IIIa. Biochemical analysis of the equine molecule using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) revealed bands of 24, 100 and 110 kDa under reducing conditions and 115 and 80 kDa under nonreducing conditions. Biochemical analysis of ruminant antigen revealed that the 24 kDa band did not appear owing to the absence of labelling with sulpho-NHS-biotin. Co.35E4 and Co.20A1 recognised two different Ca2+ complex independent epitopes. The glycoprotein IIb/IIIa was present on ruminant and equine granulocytes, monocytes and platelets. However, binding on granulocytes and monocytes was due to the adsorption of membrane platelet fragments.
Publication Date: 1995-02-01 PubMed ID: 7538249DOI: 10.1016/0165-2427(94)05310-oGoogle Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article focuses on characterising a type of membrane receptor, similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61), found on the platelets and peripheral blood leukocytes of ruminants and equines.
Identification of Monoclonal Antibodies
- The researchers defined two murine monoclonal antibodies named Co.35E4 and Co.2oA1 in the beginning of the research. These antibodies have been associated with anti-glycoprotein IIb/IIIa or CD41/61, making them suitable for the investigation.
- The antigen detected through these antibodies corresponded with ruminant and equine glycoprotein IIb/IIIa in terms of cellular distribution and apparent molecular weight. This verified their relevance to the study.
Biochemical Analysis of Equine and Ruminant Molecules
- A biochemical analysis was carried out on the equine molecule using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). This analysis displayed bands of 24, 100 and 110 kDa under reducing conditions and bands of 115 and 80 kDa under non-reducing conditions. The band pattern suggests the presence of certain proteins in the sample.
- The biochemical analysis of the ruminant antigen differed as the 24 kDa band was notably absent due to the lack of labelling with sulpho-NHS-biotin, a common biological labelling agent.
Recognition of Epitopes and Binding Sites
- Both Co.35E4 and Co.20A1 identified two different independent epitopes that are not influenced by Ca2+ complexes. Epitopes are the parts of an antigen molecule to which an antibody attaches itself.
- The research established that the glycoprotein IIb/IIIa is present on equine and ruminant granulocytes, monocytes and platelets. Granulocytes and monocytes are types of white blood cells, while platelets are small blood cells that help the body form clots to stop bleeding.
- The research also indicated that the binding on granulocytes and monocytes might be a result of the adsorption of membrane platelet fragments. Adsorption is the process through which atoms, ions or molecules bind to a surface.
Cite This Article
APA
Pintado CO, Friend M, Llanes D.
(1995).
Characterisation of a membrane receptor on ruminants and equine platelets and peripheral blood leukocytes similar to the human integrin receptor glycoprotein IIb/IIIa (CD41/61).
Vet Immunol Immunopathol, 44(3-4), 359-368.
https://doi.org/10.1016/0165-2427(94)05310-o Publication
Researcher Affiliations
- Dpto de Genética, Facultad de Veterinaria, Universidad de Córdoba, Spain.
MeSH Terms
- Animals
- Antibodies, Monoclonal / biosynthesis
- Antigens, CD / analysis
- Blood Platelets / chemistry
- Epitopes
- Horses / blood
- Leukocytes / chemistry
- Mice
- Platelet Membrane Glycoproteins / analysis
- Platelet Membrane Glycoproteins / immunology
- Receptors, Cell Surface / analysis
- Ruminants / blood
- Species Specificity
Citations
This article has been cited 2 times.- Lin GL, Chang HH, Lien TS, Chen PK, Chan H, Su MT, Liao CY, Sun DS. Suppressive effect of dengue virus envelope protein domain III on megakaryopoiesis.. Virulence 2017 Nov 17;8(8):1719-1731.
- Chen PK, Chang HH, Lin GL, Wang TP, Lai YL, Lin TK, Hsieh MC, Kau JH, Huang HH, Hsu HL, Liao CY, Sun DS. Suppressive effects of anthrax lethal toxin on megakaryopoiesis.. PLoS One 2013;8(3):e59512.
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