Characteristics of equine mesenchymal stem cells derived from amnion and bone marrow: in vitro proliferative and multilineage potential assessment.
Abstract: This is the first study comparing stemness features of equine mesenchymal progenitor cells derived from amniotic membrane and bone marrow. Objective: To investigate an alternative and noninvasive stromal cell source for equine tissue engineering. Methods: In vitro experimental study of the characteristics of equine mesenchymal progenitor cells derived from amnion and bone marrow. Methods: Cells isolated from amniotic membrane and bone marrow were analysed for proliferation (growth curve, doubling time, colony forming unit). Immunocytochemical detection of pluripotency markers and gene expression of stromal cell markers were also performed and these cells were studied for multilineage plasticity. Results: Amniotic stromal cells (AMSCs) and bone marrow mesenchymal cells (BM-MSCs) both exhibited mature stromal cell-specific gene expression and immunocytochemical properties, but showed substantial differences in their proliferative and differentiation potential. The mean doubling time for AMSCs was significantly lower (P<0.05) than that observed for BM-MSCs (1.17 ± 0.15 vs. 3.27 ± 0.19 days, respectively). Compared to AMSCs, BM-MSCs also demonstrated a significantly (P<0.05) lower clonogenic capability (one fibroblast-like colony forming unit from a mean of 590.15 cells seeded for BM-MSCs vs. 242.73 cells seeded for AMSCs). BM-MSCs did not differentiate into glial cells, and the osteogenic differentiation process was longer than for AMSCs. Conclusions: The amniotic membrane could be a valuable source of MSCs to be used both for allogenic and/or autologous therapies. The noninvasive nature and low cost of collection, the rapid proliferation along with a greater differentiation potential and the 'off the shelf' preparation potential could make AMCs useful for cell therapy.
© 2013 EVJ Ltd.
Publication Date: 2013-03-26 PubMed ID: 23527626DOI: 10.1111/evj.12052Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article analyzes and compares the stem cell attributes of equine mesenchymal progenitor cells sourced from the amniotic membrane and the bone marrow. It highlights the potential of amniotic membrane as a noninvasive, effective source of progenitor cells for equine tissue engineering.
Research Methodology
- The study was conducted in vitro, studying the attributes of equine mesenchymal progenitor cells derived from two sources: the amnion and bone marrow.
- Methods involved the isolation of cells from the mentioned sources and subsequent analysis of their proliferation factors like growth curve, doubling time, and the ability to form a colony.
- Techniques like immunocytochemical detection were used to identify pluripotency markers and the gene expression of stromal cell markers.
- The study also monitored the cells for multilineage plasticity, meaning their capacity to differentiate into more than one cell type under the right conditions.
Results
- The findings revealed that both Amniotic stromal cells (AMSCs) and bone marrow mesenchymal cells (BM-MSCs) displayed mature stromal cell-specific gene expression and immunocytochemical properties. However, there were significant differences in their rate of proliferation and differentiation potential.
- The doubling time for AMSCs was on average significantly lower than that of BM-MSCs.
- BM-MSCs exhibited a lower clonogenic ability compared to AMSCs, meaning that they had a reduced capacity to form colonies.
- Unlike AMSCs, BM-MSCs were not able to differentiate into glial cells and the process of osteogenic differentiation was longer than for AMSCs.
- As such, the study found AMSCs to have a more prolific and varied differentiation potential.
Conclusions
- The research concluded that amniotic membrane could potentially be a valuable source of MSCs for equine tissue engineering.
- Due to its noninvasive nature, low collection cost, swift proliferation rate combined with a richer differentiation potential, AMSCs could be very useful for cell therapy.
- The amniotic membrane’s potential for ‘off the shelf’ preparation is an added advantage rendering these cells suitable for flexible and efficient use in medical therapies.
Cite This Article
APA
Lange-Consiglio A, Corradetti B, Meucci A, Perego R, Bizzaro D, Cremonesi F.
(2013).
Characteristics of equine mesenchymal stem cells derived from amnion and bone marrow: in vitro proliferative and multilineage potential assessment.
Equine Vet J, 45(6), 737-744.
https://doi.org/10.1111/evj.12052 Publication
Researcher Affiliations
- Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Italy.
MeSH Terms
- Amnion / cytology
- Animals
- Bone Marrow Cells
- Cell Differentiation
- Cell Proliferation
- Cells, Cultured
- Gene Expression Regulation / physiology
- Horses
- Mesenchymal Stem Cells / cytology
- Mesenchymal Stem Cells / physiology
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Staining and Labeling
Citations
This article has been cited 18 times.- Orlandin JR, Gomes IDS, Sallum Leandro SF, Fuertes Cagnim A, Casals JB, Carregaro AB, Freitas SH, Machado LC, Reis Castiglioni MC, Garcia Alves AL, de Vasconcelos Machado VM, Ambrósio CE. Treatment of Chronic Spinal Cord Injury in Dogs Using Amniotic Membrane-Derived Stem Cells: Preliminary Results.. Stem Cells Cloning 2021;14:39-49.
- Ribitsch I, Oreff GL, Jenner F. Regenerative Medicine for Equine Musculoskeletal Diseases.. Animals (Basel) 2021 Jan 19;11(1).
- Ambrósio CE, Orlandin JR, Oliveira VC, Motta LCB, Pinto PAF, Pereira VM, Padoveze LR, Karam RG, Pinheiro AO. Potential application of aminiotic stem cells in veterinary medicine.. Anim Reprod 2020 May 22;16(1):24-30.
- Hutchings G, Moncrieff L, Dompe C, Janowicz K, Sibiak R, Bryja A, Jankowski M, Mozdziak P, Bukowska D, Antosik P, Shibli JA, Dyszkiewicz-Konwińska M, Bruska M, Kempisty B, Piotrowska-Kempisty H. Bone Regeneration, Reconstruction and Use of Osteogenic Cells; from Basic Knowledge, Animal Models to Clinical Trials.. J Clin Med 2020 Jan 4;9(1).
- Lange-Consiglio A, Gusmara C, Manfredi E, Idda A, Soggiu A, Greco V, Bonizzi L, Cremonesi F, Zecconi A. Antimicrobial Effects of Conditioned Medium From Amniotic Progenitor Cells in vitro and in vivo: Toward Tissue Regenerative Therapies for Bovine Mastitis.. Front Vet Sci 2019;6:443.
- Merlo B, Teti G, Lanci A, Burk J, Mazzotti E, Falconi M, Iacono E. Comparison between adult and foetal adnexa derived equine post-natal mesenchymal stem cells.. BMC Vet Res 2019 Aug 2;15(1):277.
- Lanci A, Merlo B, Mariella J, Castagnetti C, Iacono E. Heterologous Wharton's Jelly Derived Mesenchymal Stem Cells Application on a Large Chronic Skin Wound in a 6-Month-Old Filly.. Front Vet Sci 2019;6:9.
- Shi R, Huang Y, Ma C, Wu C, Tian W. Current advances for bone regeneration based on tissue engineering strategies.. Front Med 2019 Apr;13(2):160-188.
- Barboni B, Russo V, Berardinelli P, Mauro A, Valbonetti L, Sanyal H, Canciello A, Greco L, Muttini A, Gatta V, Stuppia L, Mattioli M. Placental Stem Cells from Domestic Animals: Translational Potential and Clinical Relevance.. Cell Transplant 2018 Jan;27(1):93-116.
- Deng Y, Huang G, Zou L, Nong T, Yang X, Cui J, Wei Y, Yang S, Shi D. Isolation and characterization of buffalo (bubalus bubalis) amniotic mesenchymal stem cells derived from amnion from the first trimester pregnancy.. J Vet Med Sci 2018 Apr 27;80(4):710-719.
- Zahedi M, Parham A, Dehghani H, Mehrjerdi HK. Stemness Signature of Equine Marrow-derived Mesenchymal Stem Cells.. Int J Stem Cells 2017 May 30;10(1):93-102.
- Perrini C, Strillacci MG, Bagnato A, Esposti P, Marini MG, Corradetti B, Bizzaro D, Idda A, Ledda S, Capra E, Pizzi F, Lange-Consiglio A, Cremonesi F. Microvesicles secreted from equine amniotic-derived cells and their potential role in reducing inflammation in endometrial cells in an in-vitro model.. Stem Cell Res Ther 2016 Nov 18;7(1):169.
- Somal A, Bhat IA, B I, Pandey S, Panda BS, Thakur N, Sarkar M, Chandra V, Saikumar G, Sharma GT. A Comparative Study of Growth Kinetics, In Vitro Differentiation Potential and Molecular Characterization of Fetal Adnexa Derived Caprine Mesenchymal Stem Cells.. PLoS One 2016;11(6):e0156821.
- Fülber J, Maria DA, da Silva LC, Massoco CO, Agreste F, Baccarin RY. Comparative study of equine mesenchymal stem cells from healthy and injured synovial tissues: an in vitro assessment.. Stem Cell Res Ther 2016 Mar 5;7:35.
- Tessier L, Bienzle D, Williams LB, Koch TG. Phenotypic and immunomodulatory properties of equine cord blood-derived mesenchymal stromal cells.. PLoS One 2015;10(4):e0122954.
- Mohanty N, Gulati BR, Kumar R, Gera S, Kumar S, Kumar P, Yadav PS. Phenotypical and functional characteristics of mesenchymal stem cells derived from equine umbilical cord blood.. Cytotechnology 2016 Aug;68(4):795-807.
- Corradetti B, Correani A, Romaldini A, Marini MG, Bizzaro D, Perrini C, Cremonesi F, Lange-Consiglio A. Amniotic membrane-derived mesenchymal cells and their conditioned media: potential candidates for uterine regenerative therapy in the horse.. PLoS One 2014;9(10):e111324.
- Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
