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Equine veterinary journal2013; 45(6); 737-744; doi: 10.1111/evj.12052

Characteristics of equine mesenchymal stem cells derived from amnion and bone marrow: in vitro proliferative and multilineage potential assessment.

Abstract: This is the first study comparing stemness features of equine mesenchymal progenitor cells derived from amniotic membrane and bone marrow. Objective: To investigate an alternative and noninvasive stromal cell source for equine tissue engineering. Methods: In vitro experimental study of the characteristics of equine mesenchymal progenitor cells derived from amnion and bone marrow. Methods: Cells isolated from amniotic membrane and bone marrow were analysed for proliferation (growth curve, doubling time, colony forming unit). Immunocytochemical detection of pluripotency markers and gene expression of stromal cell markers were also performed and these cells were studied for multilineage plasticity. Results: Amniotic stromal cells (AMSCs) and bone marrow mesenchymal cells (BM-MSCs) both exhibited mature stromal cell-specific gene expression and immunocytochemical properties, but showed substantial differences in their proliferative and differentiation potential. The mean doubling time for AMSCs was significantly lower (P<0.05) than that observed for BM-MSCs (1.17 ± 0.15 vs. 3.27 ± 0.19 days, respectively). Compared to AMSCs, BM-MSCs also demonstrated a significantly (P<0.05) lower clonogenic capability (one fibroblast-like colony forming unit from a mean of 590.15 cells seeded for BM-MSCs vs. 242.73 cells seeded for AMSCs). BM-MSCs did not differentiate into glial cells, and the osteogenic differentiation process was longer than for AMSCs. Conclusions: The amniotic membrane could be a valuable source of MSCs to be used both for allogenic and/or autologous therapies. The noninvasive nature and low cost of collection, the rapid proliferation along with a greater differentiation potential and the 'off the shelf' preparation potential could make AMCs useful for cell therapy.
© 2013 EVJ Ltd.
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Publication Date: 2013-03-26 PubMed ID: 23527626DOI: 10.1111/evj.12052Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article analyzes and compares the stem cell attributes of equine mesenchymal progenitor cells sourced from the amniotic membrane and the bone marrow. It highlights the potential of amniotic membrane as a noninvasive, effective source of progenitor cells for equine tissue engineering.

Research Methodology

  • The study was conducted in vitro, studying the attributes of equine mesenchymal progenitor cells derived from two sources: the amnion and bone marrow.
  • Methods involved the isolation of cells from the mentioned sources and subsequent analysis of their proliferation factors like growth curve, doubling time, and the ability to form a colony.
  • Techniques like immunocytochemical detection were used to identify pluripotency markers and the gene expression of stromal cell markers.
  • The study also monitored the cells for multilineage plasticity, meaning their capacity to differentiate into more than one cell type under the right conditions.

Results

  • The findings revealed that both Amniotic stromal cells (AMSCs) and bone marrow mesenchymal cells (BM-MSCs) displayed mature stromal cell-specific gene expression and immunocytochemical properties. However, there were significant differences in their rate of proliferation and differentiation potential.
  • The doubling time for AMSCs was on average significantly lower than that of BM-MSCs.
  • BM-MSCs exhibited a lower clonogenic ability compared to AMSCs, meaning that they had a reduced capacity to form colonies.
  • Unlike AMSCs, BM-MSCs were not able to differentiate into glial cells and the process of osteogenic differentiation was longer than for AMSCs.
  • As such, the study found AMSCs to have a more prolific and varied differentiation potential.

Conclusions

  • The research concluded that amniotic membrane could potentially be a valuable source of MSCs for equine tissue engineering.
  • Due to its noninvasive nature, low collection cost, swift proliferation rate combined with a richer differentiation potential, AMSCs could be very useful for cell therapy.
  • The amniotic membrane’s potential for ‘off the shelf’ preparation is an added advantage rendering these cells suitable for flexible and efficient use in medical therapies.

Cite This Article

APA
Lange-Consiglio A, Corradetti B, Meucci A, Perego R, Bizzaro D, Cremonesi F. (2013). Characteristics of equine mesenchymal stem cells derived from amnion and bone marrow: in vitro proliferative and multilineage potential assessment. Equine Vet J, 45(6), 737-744. https://doi.org/10.1111/evj.12052

Publication

Equine veterinary journal
ISSN: 2042-3306
NlmUniqueID: 0173320
Country: United States
Language: English
Volume: 45
Issue: 6
Pages: 737-744

Researcher Affiliations

Lange-Consiglio, A
  • Large Animal Hospital, Reproduction Unit, Università degli Studi di Milano, Italy.
Corradetti, B
    Meucci, A
      Perego, R
        Bizzaro, D
          Cremonesi, F

            MeSH Terms

            • Amnion / cytology
            • Animals
            • Bone Marrow Cells
            • Cell Differentiation
            • Cell Proliferation
            • Cells, Cultured
            • Gene Expression Regulation / physiology
            • Horses
            • Mesenchymal Stem Cells / cytology
            • Mesenchymal Stem Cells / physiology
            • Reverse Transcriptase Polymerase Chain Reaction / veterinary
            • Staining and Labeling

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