FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / Vet Microbiol / Characteristics of extended-spectrum cephalosporin-resistant...
Veterinary microbiology2007; 124(3-4); 248-255; doi: 10.1016/j.vetmic.2007.04.027

Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates from horses.

Abstract: The aim of the present study was to contribute to the knowledge on extended-spectrum beta-lactamases (ESBL's), AmpC beta-lactamases and integrons in Enterobacteriaceae isolated from horses, which is still limited. The susceptibility of 1581 clinical isolates from animals to ceftiofur was tested. Most of these isolates (n=1347) originated from horses. Seven ceftiofur-resistant equine isolates (four Escherichia coli and three Klebsiella pneumoniae) were identified and all seven were multidrug-resistant. These isolates were further studied for the presence of ESBL's, AmpC beta-lactamases and class 1 integrons. The potential for the horizontal transfer of resistance genes among these clinical isolates was also studied. ESBL-type resistance genes were found in five isolates, AmpC-type genes in one isolates and integrons in six isolates. Nucleotide sequence analysis revealed that the isolates carried the bla(CTX-M-1), bla(CMY-2), bla(TEM-1) and/or bla(SHV-1) genes. This is the first report describing the in vitro conjugal transfer of the bla(CTX-M-1) genes from a clinical E. coli isolate to Salmonella isolates. Gene cassettes encoding resistance to aminoglycosides (aadA1, aadA2 and aadA5), and trimethoprim (dfrA1, drfA12 and dfrA17) were found on the integrons present in the isolates. The cassette arrays of the dfrA17-aadA5 and dfrA1-aadA1 genes in the two integrons of a single E. coli isolate have not yet been described before. To our knowledge this is the first report on ESBL's and AmpC beta-lactamases in equine E. coli and Klebsiella isolates.
Get Full Text
Publication Date: 2007-04-24 PubMed ID: 17521833DOI: 10.1016/j.vetmic.2007.04.027Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study researches on a particular type of antibiotic resistance in bacteria found in horses, with focus on extended-spectrum beta-lactamases (ESBL’s), AmpC beta-lactamases and integrins in Enterobacteriaceae. Seven resistant pathogens identified from these tests possess multiple antibiotic resistances and carry genetic resistance that could be potentially transferable.

Objective and Methodology of the Research

  • The goal of the investigation was to enhance understanding of ESBL’s, AmpC beta-lactamases and class 1 integrons within Enterobacteriaceae sourced from horses.
  • The researchers scrutinized 1581 clinical isolates obtained from animals to confirm their susceptibility to the antibiotic ceftiofur. Out of these, the majority (1347) came from horses.
  • They discovered seven equine isolates resistant to ceftiofur (four Escherichia coli and three Klebsiella pneumoniae) which were also multi-drug resistant. These seven types were further examined for ESBL’s, AmpC beta-lactamases, class 1 integrons and their potential for horizontal gene transfer.

Findings of the Study

  • Five isolates had ESBL-type resistant genes, one had AmpC-type, and six carried class 1 integrons. Detailed analysis revealed these isolates bore the resistance genes bla(CTX-M-1), bla(CMY-2), bla(TEM-1), and/or bla(SHV-1).
  • This research revealed the first identified incident of in vitro horizontal transfer of bla(CTX-M-1) resistance genes from E. coli clinical isolates to Salmonella isolates.
  • Gene cassettes that code resistance to aminoglycosides (aadA1, aadA2, aadA5) and trimethoprim (dfrA1, drfA12, dfrA17) were found nested within the integrons of the isolates.
  • Two unique cassette arrangements, dfrA17-aadA5 and dfrA1-aadA1, lodged within the integrons of a singular E. coli isolate, were recounted for the first time.

Significance of the Research

  • This was the first known report on extended-spectrum beta-lactamases (ESBL’s) and AmpC beta-lactamases in equine E. coli and Klebsiella pneumoniae isolates.
  • The research highlights the potential threat of multiresistant bacteria in animals, and the potential for these pathogens to transfer resistance to other bacteria.

Cite This Article

APA
Vo AT, van Duijkeren E, Fluit AC, Gaastra W. (2007). Characteristics of extended-spectrum cephalosporin-resistant Escherichia coli and Klebsiella pneumoniae isolates from horses. Vet Microbiol, 124(3-4), 248-255. https://doi.org/10.1016/j.vetmic.2007.04.027

Publication

Veterinary microbiology
ISSN: 0378-1135
NlmUniqueID: 7705469
Country: Netherlands
Language: English
Volume: 124
Issue: 3-4
Pages: 248-255

Researcher Affiliations

Vo, An T T
  • Department of Infectious Diseases and Immunology, Faculty of Veterinary Medicine, Utrecht University, PO Box 80165, 3508 TD Utrecht, The Netherlands.
van Duijkeren, Engeline
    Fluit, Ad C
      Gaastra, Wim

        MeSH Terms

        • Animals
        • Anti-Bacterial Agents / pharmacology
        • Cephalosporin Resistance / genetics
        • Cephalosporins / pharmacology
        • Conjugation, Genetic
        • DNA, Bacterial / chemistry
        • DNA, Bacterial / genetics
        • Drug Resistance, Multiple, Bacterial / genetics
        • Escherichia coli / drug effects
        • Escherichia coli Infections / microbiology
        • Escherichia coli Infections / veterinary
        • Female
        • Gene Transfer, Horizontal
        • Horse Diseases / microbiology
        • Horses
        • Klebsiella Infections / microbiology
        • Klebsiella Infections / veterinary
        • Klebsiella pneumoniae / drug effects
        • Male
        • Microbial Sensitivity Tests / veterinary
        • Molecular Sequence Data
        • beta-Lactamases / genetics

        Citations

        This article has been cited 20 times.
        1. Tyrnenopoulou P, Fthenakis GC. Clinical Aspects of Bacterial Distribution and Antibiotic Resistance in the Reproductive System of Equids.. Antibiotics (Basel) 2023 Mar 28;12(4).
          doi: 10.3390/antibiotics12040664pubmed: 37107026google scholar: lookup
        2. Gehlen H, Klein KS, Merle R, Lübke-Becker A, Stoeckle SD. Does colonization with MRSA, ESBL - producing Enterobacteriaceae, and/or Acinetobacter baumannii - increase the risk for postoperative surgical site infection?. Vet Med Sci 2023 Mar;9(2):729-737.
          doi: 10.1002/vms3.1073pubmed: 36646070google scholar: lookup
        3. Sukmawinata E, Uemura R, Sato W, Thu Htun M, Sueyoshi M. Multidrug-Resistant ESBL/AmpC-Producing Klebsiella pneumoniae Isolated from Healthy Thoroughbred Racehorses in Japan.. Animals (Basel) 2020 Feb 25;10(3).
          doi: 10.3390/ani10030369pubmed: 32106501google scholar: lookup
        4. Loncaric I, Cabal Rosel A, Szostak MP, Licka T, Allerberger F, Ruppitsch W, Spergser J. Broad-Spectrum Cephalosporin-Resistant Klebsiella spp. Isolated from Diseased Horses in Austria.. Animals (Basel) 2020 Feb 20;10(2).
          doi: 10.3390/ani10020332pubmed: 32093201google scholar: lookup
        5. Shnaiderman-Torban A, Navon-Venezia S, Dor Z, Paitan Y, Arielly H, Ahmad WA, Kelmer G, Fulde M, Steinman A. Extended-Spectrum β-lactamase-Producing Enterobacteriaceae Shedding in Farm Horses Versus Hospitalized Horses: Prevalence and Risk Factors.. Animals (Basel) 2020 Feb 11;10(2).
          doi: 10.3390/ani10020282pubmed: 32054111google scholar: lookup
        6. Hordijk J, Farmakioti E, Smit LAM, Duim B, Graveland H, Theelen MJP, Wagenaar JA. Fecal Carriage of Extended-Spectrum-β-Lactamase/AmpC-Producing Escherichia coli in Horses.. Appl Environ Microbiol 2020 Apr 1;86(8).
          doi: 10.1128/AEM.02590-19pubmed: 32033947google scholar: lookup
        7. Trigo da Roza F, Couto N, Carneiro C, Cunha E, Rosa T, Magalhães M, Tavares L, Novais Â, Peixe L, Rossen JW, Lamas LP, Oliveira M. Commonality of Multidrug-Resistant Klebsiella pneumoniae ST348 Isolates in Horses and Humans in Portugal.. Front Microbiol 2019;10:1657.
          doi: 10.3389/fmicb.2019.01657pubmed: 31379799google scholar: lookup
        8. Kaspar U, von Lützau K, Schlattmann A, Rösler U, Köck R, Becker K. Zoonotic multidrug-resistant microorganisms among non-hospitalized horses from Germany.. One Health 2019 Jun;7:100091.
          doi: 10.1016/j.onehlt.2019.100091pubmed: 31016221google scholar: lookup
        9. Daehre K, Projahn M, Friese A, Semmler T, Guenther S, Roesler UH. ESBL-Producing Klebsiella pneumoniae in the Broiler Production Chain and the First Description of ST3128.. Front Microbiol 2018;9:2302.
          doi: 10.3389/fmicb.2018.02302pubmed: 30337912google scholar: lookup
        10. Wolny-Koładka K, Lenart-Boroń A. Antimicrobial resistance and the presence of extended-spectrum beta-lactamase genes in Escherichia coli isolated from the environment of horse riding centers.. Environ Sci Pollut Res Int 2018 Aug;25(22):21789-21800.
          doi: 10.1007/s11356-018-2274-xpubmed: 29796881google scholar: lookup
        11. Chang SK, Lo DY, Wei HW, Kuo HC. Antimicrobial resistance of Escherichia coli isolates from canine urinary tract infections.. J Vet Med Sci 2015 Jan;77(1):59-65.
          doi: 10.1292/jvms.13-0281pubmed: 25720807google scholar: lookup
        12. Donati V, Feltrin F, Hendriksen RS, Svendsen CA, Cordaro G, García-Fernández A, Lorenzetti S, Lorenzetti R, Battisti A, Franco A. Extended-spectrum-beta-lactamases, AmpC beta-lactamases and plasmid mediated quinolone resistance in klebsiella spp. from companion animals in Italy.. PLoS One 2014;9(3):e90564.
          doi: 10.1371/journal.pone.0090564pubmed: 24595207google scholar: lookup
        13. Domingues S, da Silva GJ, Nielsen KM. Integrons: Vehicles and pathways for horizontal dissemination in bacteria.. Mob Genet Elements 2012 Sep 1;2(5):211-223.
          doi: 10.4161/mge.22967pubmed: 23550063google scholar: lookup
        14. Domingues S, Harms K, Fricke WF, Johnsen PJ, da Silva GJ, Nielsen KM. Natural transformation facilitates transfer of transposons, integrons and gene cassettes between bacterial species.. PLoS Pathog 2012;8(8):e1002837.
          doi: 10.1371/journal.ppat.1002837pubmed: 22876180google scholar: lookup
        15. Guenther S, Ewers C, Wieler LH. Extended-Spectrum Beta-Lactamases Producing E. coli in Wildlife, yet Another Form of Environmental Pollution?. Front Microbiol 2011;2:246.
          doi: 10.3389/fmicb.2011.00246pubmed: 22203818google scholar: lookup
        16. Karczmarczyk M, Abbott Y, Walsh C, Leonard N, Fanning S. Characterization of multidrug-resistant Escherichia coli isolates from animals presenting at a university veterinary hospital.. Appl Environ Microbiol 2011 Oct;77(20):7104-12.
          doi: 10.1128/AEM.00599-11pubmed: 21856835google scholar: lookup
        17. Bryan J, Leonard N, Fanning S, Katz L, Duggan V. Antimicrobial resistance in commensal faecal Escherichia coli of hospitalised horses.. Ir Vet J 2010 Jun 1;63(6):373-9.
          doi: 10.1186/2046-0481-63-6-373pubmed: 21851747google scholar: lookup
        18. Locatelli C, Scaccabarozzi L, Pisoni G, Moroni P. CTX-M1 ESBL-producing Klebsiella pneumoniae subsp. pneumoniae isolated from cases of bovine mastitis.. J Clin Microbiol 2010 Oct;48(10):3822-3.
          doi: 10.1128/JCM.00941-10pubmed: 20720020google scholar: lookup
        19. Ahmed MO, Clegg PD, Williams NJ, Baptiste KE, Bennett M. Antimicrobial resistance in equine faecal Escherichia coli isolates from North West England.. Ann Clin Microbiol Antimicrob 2010 Apr 7;9:12.
          doi: 10.1186/1476-0711-9-12pubmed: 20374640google scholar: lookup
        20. Locatelli C, Caronte I, Scaccabarozzi L, Migliavacca R, Pagani L, Moroni P. Extended-spectrum beta-lactamase production in E. coli strains isolated from clinical bovine mastitis.. Vet Res Commun 2009 Sep;33 Suppl 1:141-4.
          doi: 10.1007/s11259-009-9263-ypubmed: 19568948google scholar: lookup
        Subscribe to our newsletter
        Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.