FREE SHIPPING over $40
OVER 10000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Dental materials journal2016; 35(6); 900-907; doi: 10.4012/dmj.2016-005

Characterization and angiogenic potential of xenogeneic bone grafting materials: Role of periodontal ligament cells.

Abstract: Adequate revascularization is a prerequisite for successful healing of periodontal bone defects. This study characterized three different xenogeneic bone grafting materials: Gen-Os of equine and porcine origins, and anorganic Bio-Oss. We also investigated their angiogenic potential. All materials were composed of poorly crystalline calcium oxide phosphate, with Bio-Oss exhibiting a carbonated phase and larger particle size and both Gen-Os showing the presence of collagen. Both Gen-Os materials significantly enhanced vascular endothelial growth factor (VEGF) secretion by PDL cells. A significant increase in endothelial cell proliferation was observed in cultures with both Gen-Os conditioned media, but not with that of Bio-Oss. Finally, angiogenesis was stimulated by both Gen-Os conditioned media as demonstrated by an increased formation of capillary-like structures. Taken together, these findings indicate an enhanced angiogenic potential of both Gen-Os bone grafting materials when applied on PDL cells, most likely by increasing VEGF production.
Get Full Text
Publication Date: 2016-09-29 PubMed ID: 27680032DOI: 10.4012/dmj.2016-005Google Scholar: Lookup
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research study investigates the suitability of three different types of bone grafting materials, derived from horses (equine) and pigs (porcine) as well as anorganic Bio-Oss. The study found that two of the materials, Gen-Os equine and Gen-Os porcine, have enhanced ability to generate new blood vessels, which is crucial for bone healing, when applied to certain types of bone cells.

Study Materials: Xenogeneic Bone Grafting and PDL Cells

  • This study used three different xenogeneic bone grafting materials: Gen-Os of equine and porcine origins, and anorganic Bio-Oss. Xenogeneic materials are those sourced from a different species. In this case, Gen-Os materials are derived from horses and pigs, while Bio-Oss is an anorganic material with no animal or human origin.
  • Composed of poorly crystalline calcium oxide phosphate, all three materials were observed for their individual characteristics. Bio-Oss was identified to have a carbonated phase and larger particle size, while both Gen-Os materials showed the presence of collagen.
  • Periodontal ligament (PDL) cells were used in conjunction with these materials, as their role plays a major part in healing of bone defects. VEGF, or vascular endothelial growth factor, secretion by these cells was monitored, as it is key in the formation of new blood vessels.

Findings: Enhancement of Angiogenesis

  • Looking at the angiogenic potential, or ability to form new blood vessels, of these materials, significant enhancement was observed with both Gen-Os materials.
  • The secretion of VEGF by PDL cells was significantly increased when these cells were exposed to Gen-Os materials. This indicates that the Gen-Os materials are likely aiding in the production of this important growth factor.
  • Significant increase in endothelial cell proliferation, essentially the growth and multiplication of these vital blood vessel cells, was observed in cultures with both Gen-Os conditioned media. No such increase was observed with Bio-Oss conditioned media.
  • Stimulation of angiogenesis was also demonstrated by increased formation of capillary-like structures when both Gen-Os conditioned media were used.

Conclusions: Potential of Gen-Os Materials

  • Taken together, these findings suggest an enhanced angiogenic potential of both Gen-Os bone grafting materials when applied on PDL cells.
  • This result is most likely due to the potential of these materials to increase VEGF production, which is essential for revascularization and successful healing of bone defects.
  • It suggests that Gen-Os bone grafting materials of both equine and porcine origins could be an effective tool for promoting healing in periodontal bone defects.

Cite This Article

APA
Rombouts C, Jeanneau C, Camilleri J, Laurent P, About I. (2016). Characterization and angiogenic potential of xenogeneic bone grafting materials: Role of periodontal ligament cells. Dent Mater J, 35(6), 900-907. https://doi.org/10.4012/dmj.2016-005

Publication

Dental materials journal
ISSN: 1881-1361
NlmUniqueID: 8309299
Country: Japan
Language: English
Volume: 35
Issue: 6
Pages: 900-907

Researcher Affiliations

Rombouts, Charlotte
  • Aix Marseille Univ, CNRS, ISM, Inst Movement Sci.
Jeanneau, Charlotte
    Camilleri, Josette
      Laurent, Patrick
        About, Imad

          MeSH Terms

          • Animals
          • Bone Transplantation
          • Collagen
          • Culture Media, Conditioned
          • Horses
          • Neovascularization, Physiologic
          • Periodontal Ligament
          • Swine
          • Vascular Endothelial Growth Factor A

          Citations

          This article has been cited 11 times.
          1. Romasco T, Tumedei M, Inchingolo F, Pignatelli P, Montesani L, Iezzi G, Petrini M, Piattelli A, Di Pietro N. A Narrative Review on the Effectiveness of Bone Regeneration Procedures with OsteoBiol(®) Collagenated Porcine Grafts: The Translational Research Experience over 20 Years. J Funct Biomater 2022 Aug 18;13(3).
            doi: 10.3390/jfb13030121pubmed: 35997459google scholar: lookup
          2. Xue N, Ding X, Huang R, Jiang R, Huang H, Pan X, Min W, Chen J, Duan JA, Liu P, Wang Y. Bone Tissue Engineering in the Treatment of Bone Defects. Pharmaceuticals (Basel) 2022 Jul 17;15(7).
            doi: 10.3390/ph15070879pubmed: 35890177google scholar: lookup
          3. Janjić K, Agis H, Moritz A, Rausch-Fan X, Andrukhov O. Effects of collagen membranes and bone substitute differ in periodontal ligament cell microtissues and monolayers. J Periodontol 2022 May;93(5):697-708.
            doi: 10.1002/JPER.21-0225pubmed: 34223638google scholar: lookup
          4. Mancini L, Romandini M, Fratini A, Americo LM, Panda S, Marchetti E. Biomaterials for Periodontal and Peri-Implant Regeneration. Materials (Basel) 2021 Jun 15;14(12).
            doi: 10.3390/ma14123319pubmed: 34203989google scholar: lookup
          5. Falacho RI, Palma PJ, Marques JA, Figueiredo MH, Caramelo F, Dias I, Viegas C, Guerra F. Collagenated Porcine Heterologous Bone Grafts: Histomorphometric Evaluation of Bone Formation Using Different Physical Forms in a Rabbit Cancellous Bone Model. Molecules 2021 Mar 2;26(5).
            doi: 10.3390/molecules26051339pubmed: 33801547google scholar: lookup
          6. Pelaez-Cruz P, López Jornet P, Pons-Fuster E. Collagen Formulation in Xenogeneic Bone Substitutes Influences Cellular Responses in Periodontal Regeneration: An In Vitro Study. Biomimetics (Basel) 2025 Sep 10;10(9).
            doi: 10.3390/biomimetics10090608pubmed: 41002843google scholar: lookup
          7. Yamada R, Xavier SP, Nakajima Y, Silva ER, Botticelli D, Teranishi Y, Baba S. Impact of Collagenated and Non-Collagenated Deproteinized Bovine Bone Mineral on Schneiderian Membrane Integrity in Rabbits. Dent J (Basel) 2025 Jan 2;13(1).
            doi: 10.3390/dj13010019pubmed: 39851595google scholar: lookup
          8. Menini M, Canullo L, Iacono R, Triestino A, Caponio VCA, Savadori P, Pesce P, Pedetta A, Guerra F. Effect of Different Graft Material Consistencies in the Treatment of Minimal Bone Dehiscence: A Retrospective Pilot Study. Dent J (Basel) 2024 Jun 27;12(7).
            doi: 10.3390/dj12070198pubmed: 39056985google scholar: lookup
          9. Jeanneau C, Catherine JH, Giraud T, Lan R, About I. The Added Value of a Collagenated Thermosensitive Bone Substitute as a Scaffold for Bone Regeneration. Materials (Basel) 2024 Jan 27;17(3).
            doi: 10.3390/ma17030625pubmed: 38591482google scholar: lookup
          10. Maiotti M, Massoni C. Arthroscopic Xenograft With Cerclage Fixation: A Method for Glenoid Bone Loss Reconstruction With Cerclage Fixation Using a Specific Posterior Guide. Arthrosc Tech 2023 Oct;12(10):e1657-e1664.
            doi: 10.1016/j.eats.2023.05.011pubmed: 37942095google scholar: lookup
          11. Pelaez-Cruz P, Lopez-Jornet P, Pons-Fuster E. Effects of photobiomodulation on human dental pulp stem cells treated with a bone xenograft: An in vitro study. Lasers Med Sci 2026 Mar 13;41(1).
            doi: 10.1007/s10103-026-04845-0pubmed: 41824113google scholar: lookup
          Subscribe to our newsletter
          Join 99,780 horse owners like you who receive our email updates on horse health and nutrition.