Characterization and complete genome analysis of a bacteriophage vB_EcoM_DE7 infecting donkey-derived Escherichia coli.
Abstract: A lytic bacteriophage vB_EcoM_DE7 (hereafter designated DE7) that could infect donkey-derived Escherichia coli was isolated. The bacteriophage was examined by transmission electron microscopy, and the result showed that DE7 belonged to the family Myoviridae. The microbiological characterization revealed that DE7 was stable over a broad range of pHs (3 ∼10) at 40-50 °C. The latent period was 10 min, and the burst size was 43 PFUs/infected cell. The whole-genome sequencing showed that DE7 was a dsDNA virus and had a genome of 86,130 bp. The genome contained 124 predicted open reading frames (ORFs), 35 of which had known functions, including DNA replication and modification, transcriptional regulation, structural and packaging proteins, and host cell lysis. Twenty tRNA genes were identified, but no genes associated with bacterial pathogenicity, lysogeny and drug resistance were identified. BLASTN analysis revealed that phage DE7 had a high sequence identity (96%) with Salmonella phage vB_SPuM_SP116, but it could not lyse any Salmonella strain tested in this study. DE7 was classified as a Felix O1-like virus based on its general characterization and genomic information. Since phage DE7 exhibited high efficacy in lysing E. coli and lacked genes associated with bacterial virulence, antimicrobial resistance and lysogeny, it could be potentially used to control foal diarrhoea caused by E. coli.
Copyright © 2022. Published by Elsevier B.V.
Publication Date: 2022-09-05 PubMed ID: 36064043DOI: 10.1016/j.virusres.2022.198913Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This research identifies a bacteriophage (a virus that infects bacteria), it’s properties, genetic profile and potential uses in controlling disease caused by a certain bacteria in donkeys.
Identification of Bacteriophage DE7
- Researchers isolated a lytic bacteriophage named vB_EcoM_DE7 (shortened to DE7), capable of infecting donkey-derived Escherichia coli bacteria.
- Transmission electron microscopy was used to visually observe the bacteriophage and it was found to belong to the Myoviridae family.
Characterization of Bacteriophage DE7
- The bacteriophage underwent microbiological evaluation, revealing its robustness in a variety of conditions. It was found stable over a wide pH range (3 to 10) and in temperatures of 40-50 degrees Celsius.
- The latency period (the time taken to infect the bacteria) was recorded as 10 minutes with a burst size (number of new phages released per infected cell) of 43 PFUs.
Genetic Analysis of Bacteriophage DE7
- The genome of DE7 was sequenced, showing it to be a dsDNA (double-stranded DNA) virus with a genome size of 86,130 base pairs.
- 124 predicted open reading frames (ORFs) were found within the genome, with 35 of them having identifiable functions related to DNA replication and modification, transcriptional regulation, structural and packaging proteins, and host cell lysis.
- 20 tRNA genes were also identified, but the genome did not show any genes associated with bacterial pathogenicity, lysogeny and drug resistance.
- When compared to other similar bacteriophages using BLASTN analysis, DE7 showed 96% genetic sequence identity with the Salmonella phage vB_SPuM_SP116. However, DE7 did not exhibit an ability to lyse any Salmonella strains tested.
Potential Application of Bacteriophage DE7
- Based on these analysis results, DE7 was classified as a Felix O1-like virus.
- The bacteriophage demonstrated high efficacy in lysing (breaking down) E. coli and did not contain genes that could contribute to bacterial virulence or antimicrobial resistance.
- This means DE7 could potentially be used to control diarrhea caused by E. coli in foals (young donkeys).
Cite This Article
APA
Cui JQ, Liu WH, Zang YX, Zhang C, Zou L, Sun HZ, Pan Q, Ren HY.
(2022).
Characterization and complete genome analysis of a bacteriophage vB_EcoM_DE7 infecting donkey-derived Escherichia coli.
Virus Res, 321, 198913.
https://doi.org/10.1016/j.virusres.2022.198913 Publication
Researcher Affiliations
- College of Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
- College of Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
- College of Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
- College of Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
- College of Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China.
- Qingdao Phagepharm Bio-tech Co, Ltd, Qingdao, Shandong, China.
- Qingdao Phagepharm Bio-tech Co, Ltd, Qingdao, Shandong, China.
- College of Veterinary medicine, Qingdao Agricultural University, Qingdao, Shandong, China. Electronic address: renren0228@sina.com.
MeSH Terms
- Animals
- Anti-Infective Agents
- Bacteriophages / genetics
- Equidae
- Escherichia coli / genetics
- Genome, Viral
- Horses
- RNA, Transfer / genetics
Conflict of Interest Statement
Conflict of Interest The authors declare that they have no conflict of interest.
Citations
This article has been cited 5 times.- Cui JQ, Liu WH, Zhang C, Zou L, Ren HY. Genomic characterization of three bacteriophages infecting donkey-derived Escherichia coli. Virus Genes 2023 Oct;59(5):752-762.
- Cui J, Zhong W, Liu W, Zhang C, Zou L, Ren H. Whole genome sequencing and annotation of a lysogenic phage vB_EcoP_DE5 isolated from donkey-derived Escherichia coli. Virus Genes 2023 Apr;59(2):290-300.
- Tian R, Chong CJ, Bai YY, Chen N, Qiao RR, Wang K, Wang YW, Zhao P, Zhao CB, Tang YP, Zhang L, Zhang Q. The role of gut microbiota in diarrhea and its alleviation through microbiota-targeted interventions. Front Microbiol 2025;16:1630823.
- Pchelin IM, Smolensky AV, Azarov DV, Goncharov AE. Lytic Spectra of Tailed Bacteriophages: A Systematic Review and Meta-Analysis. Viruses 2024 Dec 4;16(12).
- Kobakhidze S, Koulouris S, Kakabadze N, Kotetishvili M. Genetic recombination-mediated evolutionary interactions between phages of potential industrial importance and prophages of their hosts within or across the domains of Escherichia, Listeria, Salmonella, Campylobacter, and Staphylococcus. BMC Microbiol 2024 May 4;24(1):155.
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