FREE SHIPPING over $40
OVER 9000 FIVE-STAR REVIEWS
5% OFF ALL SUBSCRIPTIONS
100% HAPPINESS GUARANTEE
Analyze Diet
0
Home / Equine Research Bank / J Biol Regul Homeost Agents / Characterization and differentiation of equine tendon-derive...
Journal of biological regulators and homeostatic agents2011; 25(2 Suppl); S75-S84;

Characterization and differentiation of equine tendon-derived progenitor cells.

Abstract: Mesenchymal stem cells have been recently investigated for their potential use in regenerative medicine. Population of adult stem cells were recently identified in human and lab animal tendons, but no detailed investigations have been made in the equine species. The aim of our study is to identify a progenitor cell population from tendon tissue (TSPCs) in the horse superficial digital flexor tendon that are able to be highly clonogenic, to grow fast and to differentiate in different induced cell lineages as well as bone marrow derived progenitor cells (BM-MSCs). The hypothesis that TSPCs possess a mesenchymal stem cell behavior opens a new prospective for tendon regenerative medicine approaches. TSPCs were expanded more rapidly and showed higher plating efficiency when compared with BM-MSCs. Both cell lines expressed identical stem cell markers in vitro and they were able to differentiate towards osteogenic and adipogenic lineages as demonstrated with cytochemical staining and mRNA gene expression. TSPCs showed a positive but limited chondrogenic differentiation compared with BM-MSCs as demonstrated by histological and biochemical analyses. According to our results, equine TSPCs have high clonogenic properties and proliferating potential, they express stem cell markers and have the capability to be multipotent as well as BM-MSCs. These findings suggest that TSPCs may represent a good model for stem cell biology and could be useful for future tendon regenerative medicine investigations.
Get Full Text
Publication Date: 2011-12-08 PubMed ID: 22051173
The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
LinkedInCopy
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research explored a specific type of stem cell (tendon-derived progenitor cells or TSPCs) found in horse tendons, studying their potential for aiding in regenerative medicine applications. The research found that these cells can grow quickly, are robust, and can differentiate into various cell types, making them a promising candidate for future studies and potential regenerative therapy.

Understanding TSPCs and Research Objectives

  • This research focuses on tendon-derived progenitor cells (TSPCs), which are adult stem cells found in tendons. The main goal of the study was to identify such cells in the superficial digital flexor tendon of horses for deeper investigation.
  • The researchers hypothesized that TSPCs might exhibit qualities similar to mesenchymal stem cells, a particular type of stem cell known for its potential in regenerative medicine.
  • They compared these TSPCs with bone marrow-derived progenitor cells (BM-MSCs), another type of Stem Cell to assess their growth rate, differentiation ability, and clonogenicity (ability to form colonies).

Key Findings and Procedures

  • The research found that TSPCs grow more rapidly and show a higher plating efficiency than BM-MSCs. Both cell types displayed identical stem cell markers, suggesting comparable properties.
  • The team used cytochemical staining and mRNA gene expression to show that TSPC and BM-MSCs can differentiate into osteogenic (bone-forming) and adipogenic (fat-forming) lineages.
  • The research also examined chondrogenic (cartilage-forming) differentiation. It was found that TSPCs had a positive but limited ability to differentiate into chondrogenic cells compared to BM-MSCs.

Conclusion and Implications

  • The research concluded that equine TSPCs have promising characteristics for stem cell biology and potential applications in regenerative medicine. These include high clonogenic properties, proliferating potential, the expression of stem cell markers, and the capability for multipotency (the ability to differentiate into several cell types).
  • This discovery is significant because it potentially opens up new avenues for treatment in both veterinary and human medicine. Further exploration of TSPCs could lead to new breakthroughs in tendon regenerative medicine.

Cite This Article

APA
Lovati AB, Corradetti B, Lange Consiglio A, Recordati C, Bonacina E, Bizzaro D, Cremonesi F. (2011). Characterization and differentiation of equine tendon-derived progenitor cells. J Biol Regul Homeost Agents, 25(2 Suppl), S75-S84.

Publication

Journal of biological regulators and homeostatic agents
ISSN: 0393-974X
NlmUniqueID: 8809253
Country: Italy
Language: English
Volume: 25
Issue: 2 Suppl
Pages: S75-S84

Researcher Affiliations

Lovati, A B
  • Università degli Studi di Milano, Department of Veterinary Clinical Science, Lodi, Italy. arianna.lovati@grupposandonato.it
Corradetti, B
    Lange Consiglio, A
      Recordati, C
        Bonacina, E
          Bizzaro, D
            Cremonesi, F

              MeSH Terms

              • Animals
              • Antigens, Differentiation / biosynthesis
              • Cell Differentiation
              • Cell Separation
              • Cells, Cultured
              • Chondrogenesis
              • Humans
              • Osteogenesis
              • Sheep
              • Stem Cells / cytology
              • Stem Cells / metabolism
              • Tendons / cytology
              • Tendons / metabolism

              Citations

              This article has been cited 33 times.
              1. Jiang L, Lu J, Chen Y, Lyu K, Long L, Wang X, Liu T, Li S. Mesenchymal stem cells: An efficient cell therapy for tendon repair (Review).. Int J Mol Med 2023 Aug;52(2).
                doi: 10.3892/ijmm.2023.5273pubmed: 37387410google scholar: lookup
              2. Quintero D, Perucca Orfei C, Kaplan LD, de Girolamo L, Best TM, Kouroupis D. The roles and therapeutic potentialof mesenchymal stem/stromal cells and their extracellular vesicles in tendinopathies.. Front Bioeng Biotechnol 2023;11:1040762.
                doi: 10.3389/fbioe.2023.1040762pubmed: 36741745google scholar: lookup
              3. Xue Z, Chen Z, Wu T, Li R, Chen C, Liu J, Hou H, Zheng X, Wang H. VEGFA-Enriched Exosomes from Tendon-Derived Stem Cells Facilitate Tenocyte Differentiation, Migration, and Transition to a Fibroblastic Phenotype.. Biomed Res Int 2022;2022:8537959.
                doi: 10.1155/2022/8537959pubmed: 36119932google scholar: lookup
              4. Chen Z, Chen P, Zheng M, Gao J, Liu D, Wang A, Zheng Q, Leys T, Tai A, Zheng M. Challenges and perspectives of tendon-derived cell therapy for tendinopathy: from bench to bedside.. Stem Cell Res Ther 2022 Sep 2;13(1):444.
                doi: 10.1186/s13287-022-03113-6pubmed: 36056395google scholar: lookup
              5. Russo V, El Khatib M, Prencipe G, Citeroni MR, Faydaver M, Mauro A, Berardinelli P, Cerveró-Varona A, Haidar-Montes AA, Turriani M, Di Giacinto O, Raspa M, Scavizzi F, Bonaventura F, Stöckl J, Barboni B. Tendon Immune Regeneration: Insights on the Synergetic Role of Stem and Immune Cells during Tendon Regeneration.. Cells 2022 Jan 27;11(3).
                doi: 10.3390/cells11030434pubmed: 35159244google scholar: lookup
              6. Roberts JH, Halper J. Growth Factor Roles in Soft Tissue Physiology and Pathophysiology.. Adv Exp Med Biol 2021;1348:139-159.
                doi: 10.1007/978-3-030-80614-9_6pubmed: 34807418google scholar: lookup
              7. Ning LJ, Zhang YJ, Zhang YJ, Zhu M, Ding W, Jiang YL, Zhang Y, Luo JC, Qin TW. Enhancement of Migration and Tenogenic Differentiation of Macaca Mulatta Tendon-Derived Stem Cells by Decellularized Tendon Hydrogel.. Front Cell Dev Biol 2021;9:651583.
                doi: 10.3389/fcell.2021.651583pubmed: 33987178google scholar: lookup
              8. Li Y, Wu T, Liu S. Identification and Distinction of Tenocytes and Tendon-Derived Stem Cells.. Front Cell Dev Biol 2021;9:629515.
                doi: 10.3389/fcell.2021.629515pubmed: 33937230google scholar: lookup
              9. Quam VG, Altmann NN, Brokken MT, Durgam SS. Zonal characterization and differential trilineage potentials of equine intrasynovial deep digital flexor tendon-derived cells.. BMC Vet Res 2021 Apr 1;17(1):138.
                doi: 10.1186/s12917-021-02793-1pubmed: 33794882google scholar: lookup
              10. Desai S, Jayasuriya CT. Implementation of Endogenous and Exogenous Mesenchymal Progenitor Cells for Skeletal Tissue Regeneration and Repair.. Bioengineering (Basel) 2020 Aug 4;7(3).
                doi: 10.3390/bioengineering7030086pubmed: 32759659google scholar: lookup
              11. Lin Y, Zhang L, Liu NQ, Yao Q, Van Handel B, Xu Y, Wang C, Evseenko D, Wang L. In vitro behavior of tendon stem/progenitor cells on bioactive electrospun nanofiber membranes for tendon-bone tissue engineering applications.. Int J Nanomedicine 2019;14:5831-5848.
                doi: 10.2147/IJN.S210509pubmed: 31534327google scholar: lookup
              12. Lu CC, Zhang T, Reisdorf RL, Amadio PC, An KN, Moran SL, Gingery A, Zhao C. Biological analysis of flexor tendon repair-failure stump tissue: A potential recycling of tissue for tendon regeneration.. Bone Joint Res 2019 Jun;8(6):232-245.
                doi: 10.1302/2046-3758.86.BJR-2018-0239.R1pubmed: 31346451google scholar: lookup
              13. Costa-Almeida R, Calejo I, Gomes ME. Mesenchymal Stem Cells Empowering Tendon Regenerative Therapies.. Int J Mol Sci 2019 Jun 19;20(12).
                doi: 10.3390/ijms20123002pubmed: 31248196google scholar: lookup
              14. Zhang K, Hast MW, Izumi S, Usami Y, Shetye S, Akabudike N, Philp NJ, Iwamoto M, Nissim I, Soslowsky LJ, Enomoto-Iwamoto M. Modulating Glucose Metabolism and Lactate Synthesis in Injured Mouse Tendons: Treatment With Dichloroacetate, a Lactate Synthesis Inhibitor, Improves Tendon Healing.. Am J Sports Med 2018 Jul;46(9):2222-2231.
                doi: 10.1177/0363546518778789pubmed: 29927623google scholar: lookup
              15. Chen S, Deng G, Li K, Zheng H, Wang G, Yu B, Zhang K. Interleukin-6 Promotes Proliferation but Inhibits Tenogenic Differentiation via the Janus Kinase/Signal Transducers and Activators of Transcription 3 (JAK/STAT3) Pathway in Tendon-Derived Stem Cells.. Med Sci Monit 2018 Mar 16;24:1567-1573.
                doi: 10.12659/msm.908802pubmed: 29547593google scholar: lookup
              16. Yin Z, Hu JJ, Yang L, Zheng ZF, An CR, Wu BB, Zhang C, Shen WL, Liu HH, Chen JL, Heng BC, Guo GJ, Chen X, Ouyang HW. Single-cell analysis reveals a nestin(+) tendon stem/progenitor cell population with strong tenogenic potentiality.. Sci Adv 2016 Nov;2(11):e1600874.
                doi: 10.1126/sciadv.1600874pubmed: 28138519google scholar: lookup
              17. Perucca Orfei C, Lovati AB, Viganò M, Stanco D, Bottagisio M, Di Giancamillo A, Setti S, de Girolamo L. Dose-Related and Time-Dependent Development of Collagenase-Induced Tendinopathy in Rats.. PLoS One 2016;11(8):e0161590.
                doi: 10.1371/journal.pone.0161590pubmed: 27548063google scholar: lookup
              18. Yang J, Zhao Q, Wang K, Liu H, Ma C, Huang H, Liu Y. Isolation and biological characterization of tendon-derived stem cells from fetal bovine.. In Vitro Cell Dev Biol Anim 2016 Sep;52(8):846-56.
                doi: 10.1007/s11626-016-0043-zpubmed: 27130678google scholar: lookup
              19. Fernandez-Moure JS, Corradetti B, Chan P, Van Eps JL, Janecek T, Rameshwar P, Weiner BK, Tasciotti E. Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis.. Stem Cell Res Ther 2015 Oct 26;6:203.
                doi: 10.1186/s13287-015-0193-zpubmed: 26503337google scholar: lookup
              20. Zhang K, Asai S, Yu B, Enomoto-Iwamoto M. IL-1β irreversibly inhibits tenogenic differentiation and alters metabolism in injured tendon-derived progenitor cells in vitro.. Biochem Biophys Res Commun 2015 Aug 7;463(4):667-72.
                doi: 10.1016/j.bbrc.2015.05.122pubmed: 26051275google scholar: lookup
              21. Lui PP. Markers for the identification of tendon-derived stem cells in vitro and tendon stem cells in situ - update and future development.. Stem Cell Res Ther 2015 Jun 2;6(1):106.
                doi: 10.1186/s13287-015-0097-ypubmed: 26031740google scholar: lookup
              22. Williamson KA, Lee KJ, Humphreys WJ, Comerford EJ, Clegg PD, Canty-Laird EG. Restricted differentiation potential of progenitor cell populations obtained from the equine superficial digital flexor tendon (SDFT).. J Orthop Res 2015 Jun;33(6):849-58.
                doi: 10.1002/jor.22891pubmed: 25877997google scholar: lookup
              23. Sun HB, Schaniel C, Leong DJ, Wang JH. Biology and mechano-response of tendon cells: Progress overview and perspectives.. J Orthop Res 2015 Jun;33(6):785-92.
                doi: 10.1002/jor.22885pubmed: 25728946google scholar: lookup
              24. Docheva D, Müller SA, Majewski M, Evans CH. Biologics for tendon repair.. Adv Drug Deliv Rev 2015 Apr;84:222-39.
                doi: 10.1016/j.addr.2014.11.015pubmed: 25446135google scholar: lookup
              25. Xu W, Sun Y, Zhang J, Xu K, Pan L, He L, Song Y, Njunge L, Xu Z, Chiang MY, Sung KL, Chuong CM, Yang L. Perivascular-derived stem cells with neural crest characteristics are involved in tendon repair.. Stem Cells Dev 2015 Apr 1;24(7):857-68.
                doi: 10.1089/scd.2014.0036pubmed: 25381682google scholar: lookup
              26. Cadby JA, Buehler E, Godbout C, van Weeren PR, Snedeker JG. Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.. PLoS One 2014;9(3):e92474.
                doi: 10.1371/journal.pone.0092474pubmed: 24651449google scholar: lookup
              27. Gittel C, Brehm W, Burk J, Juelke H, Staszyk C, Ribitsch I. Isolation of equine multipotent mesenchymal stromal cells by enzymatic tissue digestion or explant technique: comparison of cellular properties.. BMC Vet Res 2013 Oct 29;9:221.
                doi: 10.1186/1746-6148-9-221pubmed: 24168625google scholar: lookup
              28. Tan Q, Lui PP, Lee YW. In vivo identity of tendon stem cells and the roles of stem cells in tendon healing.. Stem Cells Dev 2013 Dec 1;22(23):3128-40.
                doi: 10.1089/scd.2013.0073pubmed: 23815595google scholar: lookup
              29. Tetta C, Consiglio AL, Bruno S, Tetta E, Gatti E, Dobreva M, Cremonesi F, Camussi G. The role of microvesicles derived from mesenchymal stem cells in tissue regeneration; a dream for tendon repair?. Muscles Ligaments Tendons J 2012 Jul;2(3):212-21.
                pubmed: 23738299
              30. Muttini A, Salini V, Valbonetti L, Abate M. Stem cell therapy of tendinopathies: suggestions from veterinary medicine.. Muscles Ligaments Tendons J 2012 Jul;2(3):187-92.
                pubmed: 23738296
              31. Lui PP, Wong OT. Tendon stem cells: experimental and clinical perspectives in tendon and tendon-bone junction repair.. Muscles Ligaments Tendons J 2012 Jul;2(3):163-8.
                pubmed: 23738293
              32. Via AG, Frizziero A, Oliva F. Biological properties of mesenchymal Stem Cells from different sources.. Muscles Ligaments Tendons J 2012 Jul;2(3):154-62.
                pubmed: 23738292
              33. Lui PP. Identity of tendon stem cells--how much do we know?. J Cell Mol Med 2013 Jan;17(1):55-64.
                doi: 10.1111/jcmm.12007pubmed: 23279609google scholar: lookup
              Subscribe to our newsletter
              Join 99,344 horse owners like you who receive our email updates on horse health and nutrition.