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International journal of molecular sciences2023; 24(4); 4191; doi: 10.3390/ijms24044191

Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 against Clostridium perfringens Biofilms.

Abstract: () is one of the foremost pathogens responsible for diarrhea in foals. As antibiotic resistance increases, phages that specifically lyse bacteria are of great interest to us with regard to . In this study, a novel phage DCp1 was isolated from the sewage of a donkey farm. Phage DCp1 had a non-contractile short tail (40 nm in length) and a regular icosahedral head (46 nm in diameter). Whole-genome sequencing indicated that phage DCp1 had a linear double-stranded DNA genome with a total length of 18,555 bp and a G + C content of 28.2%. A total of 25 ORFs were identified in the genome, 6 of which had been assigned to functional genes, others were annotated to encode hypothetical proteins. The genome of phage DCp1 lacked any tRNA, virulence gene, drug resistance gene, or lysogenic gene. Phylogenetic analysis indicated that phage DCp1 belonged to the family , . Biofilm assay showed that phage DCp1 was effective in inhibiting the formation of . Phage DCp1 could completely degrade the biofilm after 5 h of interaction. The current study provides some basic information for further research on phage DCp1 and its application.
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Publication Date: 2023-02-20 PubMed ID: 36835606PubMed Central: PMC9965233DOI: 10.3390/ijms24044191Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research paper highlights the characterization and genomic analysis of a new bacteriophage, named DCp1, found in sewage waste from a donkey farm. The phage, capable of destroying the biofilms of Clostridium perfringens, a bacterial pathogen causing diarrhea in foals, is being studied as a potential alternative to antibiotics.

Details of Phage DCp1

  • The study introduces a new bacteriophage DCp1, extracted from the sewage of a farm rearing donkeys.
  • The morphology of the phage is described to have a non-contractile short tail of 40 nanometers (nm) in length and an icosahedral head with a diameter of 46 nm – a shape common to many bacteriophages.

Genomic Analysis of DCp1

  • Sequencing of the genome showed that DCp1 contained a linear double-stranded DNA genome, with a total length of 18,555 base pairs and a Guanine + Cytosine (G+C) content of 28.2%. This information helps gain understanding of the genetic makeup of the bacteriophage.
  • The genome contained 25 open reading frames (ORFs), segments of the potential protein-coding part of the phage genome. Among these, six were classified as functional genes, while others were assumed to hold codes for yet-to-be-established proteins.
  • No genes that code for transfer RNA (tRNA), virulence, drug resistance, or lysogenic activity were found within the genome. This suggests that the phage is specifically programmed to interact with and dissolve bacterial biofilms, without known adverse effects or resistance mechanisms.
  • The researchers have classified the bacteriophage DCp1 within the Siphoviridae family, based on the phylogenetic analysis. This helps us understand the phage’s evolutionary relationships to other-known phages.

Phage DCp1 against C. perfringens Biofilms

  • The ability of the bacteriophage to inhibit the formation of C. perfringens biofilms was assessed in a biofilm assay. Biofilms are often responsible for persistent infections and are highly resistant to typical antibiotic treatments.
  • The phage DCp1 was capable of entirely dissolving C. perfringens biofilms within 5 hours of interaction, indicating its potential usage in controlling the bacterial pathogen.
  • This study highlights the potential of using phage DCp1 as an alternative or addition to traditional antibiotics. However, future research needs to focus more on its possible applications, taking into consideration the mechanism of action, safety, and regulatory requirements.

Cite This Article

APA
Tang Z, Li X, Wang X, Zhang C, Zou L, Ren H, Liu W. (2023). Characterization and Genomic Analysis of a Novel Lytic Phage DCp1 against Clostridium perfringens Biofilms. Int J Mol Sci, 24(4), 4191. https://doi.org/10.3390/ijms24044191

Publication

International journal of molecular sciences
ISSN: 1422-0067
NlmUniqueID: 101092791
Country: Switzerland
Language: English
Volume: 24
Issue: 4
PII: 4191

Researcher Affiliations

Tang, Zhaohui
  • College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Li, Xiaojing
  • College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Wang, Xinwei
  • College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Zhang, Can
  • College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Zou, Ling
  • College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Ren, Huiying
  • College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.
Liu, Wenhua
  • College of Veterinary Medicine, Qingdao Agricultural University, Qingdao 266109, China.

MeSH Terms

  • Animals
  • Horses / genetics
  • Bacteriophages / genetics
  • Clostridium perfringens
  • Phylogeny
  • Genome, Viral
  • Genomics
  • Biofilms

Grant Funding

  • SDAIT-27-04 / the Donkey Industry Innovation Team Program of Modern Agricultural Technology System from Shandong Province, China

Conflict of Interest Statement

All authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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