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Home / Equine Research Bank / Am J Vet Res / Characterization and osteogenic potential of equine muscle t...
American journal of veterinary research2013; 74(5); 790-800; doi: 10.2460/ajvr.74.5.790

Characterization and osteogenic potential of equine muscle tissue- and periosteal tissue-derived mesenchymal stem cells in comparison with bone marrow- and adipose tissue-derived mesenchymal stem cells.

Abstract: To characterize equine muscle tissue- and periosteal tissue-derived cells as mesenchymal stem cells (MSCs) and assess their proliferation capacity and osteogenic potential in comparison with bone marrow- and adipose tissue-derived MSCs. Methods: Tissues from 10 equine cadavers. Methods: Cells were isolated from left semitendinosus muscle tissue, periosteal tissue from the distomedial aspect of the right tibia, bone marrow aspirates from the fourth and fifth sternebrae, and adipose tissue from the left subcutaneous region. Mesenchymal stem cells were characterized on the basis of morphology, adherence to plastic, trilineage differentiation, and detection of stem cell surface markers via immunofluorescence and flow cytometry. Mesenchymal stem cells were tested for osteogenic potential with osteocalcin gene expression via real-time PCR assay. Mesenchymal stem cell cultures were counted at 24, 48, 72, and 96 hours to determine tissue-specific MSC proliferative capacity. Results: Equine muscle tissue- and periosteal tissue-derived cells were characterized as MSCs on the basis of spindle-shaped morphology, adherence to plastic, trilineage differentiation, presence of CD44 and CD90 cell surface markers, and nearly complete absence of CD45 and CD34 cell surface markers. Muscle tissue-, periosteal tissue-, and adipose tissue-derived MSCs proliferated significantly faster than did bone marrow-derived MSCs at 72 and 96 hours. Conclusions: Equine muscle and periosteum are sources of MSCs. Equine muscle- and periosteal-derived MSCs have osteogenic potential comparable to that of equine adipose- and bone marrow-derived MSCs, which could make them useful for tissue engineering applications in equine medicine.
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Publication Date: 2013-05-01 PubMed ID: 23627394DOI: 10.2460/ajvr.74.5.790Google Scholar: Lookup
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  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research focuses on the identification and study of mesenchymal stem cells (MSCs) derived from muscle and periosteal tissues in horses, comparing them to MSCs derived from bone marrow and adipose tissues in regard to their proliferation capacity and ability to develop into bone cells.

Research Process

  • The researchers gathered tissues from 10 equine cadavers.
  • Cells derived from muscle tissue, periosteal tissue, bone marrow aspirates, and adipose tissue were isolated and studied.
  • The team characterized the MSCs based on morphology, adherence to plastic, trilineage differentiation (their ability to mature into three types of cells), and the presence of certain cell surface markers, CD44 and CD90.
  • They also checked for the near absence of other cell surface markers, CD45 and CD34.

Osteogenic Testing

  • The osteogenic potential of the MSCs (their capacity to differentiate into bone cells) was tested using the osteocalcin gene expression through a technique called real-time PCR assay.
  • They also looked into tissue-specific MSC proliferation capacity by counting the MSC cultures at 24, 48, 72, and 96 hours.

Results

  • MSCs derived from equine muscle and periosteal tissues were confirmed to have the same characteristics as MSCs in terms of shape, adherence to plastic, trilineage differentiation, and the presence or absence of certain cell surface markers.
  • They found that the muscle, periosteal, and adipose tissue-derived MSCs proliferated significantly faster than those derived from bone marrow after 72 and 96 hours.

Conclusion

  • The researchers concluded that equine muscle and periosteal tissues can be sources of MSCs.
  • The osteogenic potential of MSCs derived from muscle and periosteal tissues were comparable to those derived from bone marrow and adipose tissues.
  • This suggests that these stem cells might be useful for tissue engineering applications in equine medicine.

Cite This Article

APA
Radtke CL, Nino-Fong R, Esparza Gonzalez BP, Stryhn H, McD￮ LA. (2013). Characterization and osteogenic potential of equine muscle tissue- and periosteal tissue-derived mesenchymal stem cells in comparison with bone marrow- and adipose tissue-derived mesenchymal stem cells. Am J Vet Res, 74(5), 790-800. https://doi.org/10.2460/ajvr.74.5.790

Publication

American journal of veterinary research
ISSN: 1943-5681
NlmUniqueID: 0375011
Country: United States
Language: English
Volume: 74
Issue: 5
Pages: 790-800

Researcher Affiliations

Radtke, Catherine L
  • Department of Health Management, Atlantic Veterinary College, University of Prince Edward Island, Charlottetown, PE C1A 4P3, Canada. cradtke@upei.ca
Nino-Fong, Rodolfo
    Esparza Gonzalez, Blanca P
      Stryhn, Henrik
        McD￮, Laurie A

          MeSH Terms

          • Adipose Tissue / cytology
          • Adipose Tissue / physiology
          • Animals
          • Antigens, CD / genetics
          • Antigens, CD / metabolism
          • Bone Marrow Cells / cytology
          • Bone Marrow Cells / physiology
          • Cadaver
          • Cells, Cultured
          • Gene Expression Regulation / physiology
          • Horses
          • Mesenchymal Stem Cells / cytology
          • Mesenchymal Stem Cells / physiology
          • Muscle, Skeletal / cytology
          • Osteogenesis / physiology
          • Periosteum / cytology

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