Frontiers in veterinary science2023; 10; 1116671; doi: 10.3389/fvets.2023.1116671

Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament.

Abstract: The lifelong eruption places a great demand on the dental pulp and periodontal ligament (PDL) of horse teeth. Cells within the pulp and PDL seem to play a key role during this remodeling. Unassigned: In this study, we isolated and cultivated MSCs (medicinal signaling cells) from dental pulp, PDL and retrobulbar fat of four horses. Subsequently, we analyzed them by flow cytometry and immunohistochemistry to determine and compare their characteristics. In addition, we localized these cells within the tissue structure via immunohistochemistry of histological sections. For these analyses, several surface markers were applied. Unassigned: The described method illustrates a feasible approach to isolate and cultivate MSCs from equine dental pulp and PDL. In the flow cytometry a vast majority of cultivated cells were positive for CD90 and CD40 and negative for CD11a/18, CD45, CD105 and MHCII suggesting that these cells feature characteristics of MSCs. Immunohistochemistry of histological pulp and PDL sections showed the localization of CD90 positive cells especially in the perivascular region and the subodontoblastic layer. Unassigned: Our findings indicate that the isolation and cultivation of MSCs from equine dental pulp and PDL is feasible although an elaborate and complicated harvesting protocol is required. MSCs isolated from dental pulp and PDL are regarded as candidates for new therapeutical approaches in equine dental medicine like regeneration of periodontal lesions, enhancement of periodontal re-attachment after dental replantation and stimulation of pulp-obliteration and apexification in combination with endodontic therapies.
Publication Date: 2023-03-10 PubMed ID: 36968463PubMed Central: PMC10036573DOI: 10.3389/fvets.2023.1116671Google Scholar: Lookup
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  • Journal Article

Summary

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Researchers have successfully isolated and cultivated medicinal signaling cells (MSCs) from horse teeth and retrobulbar fat, paving the way for potential therapeutic applications in equine dental medicine.

Methodology

  • The researchers collected MSCs from the dental pulp, periodontal ligament (PDL) and retrobulbar fat of four horses for their study.
  • Post extraction, the cells were cultivated and studied using techniques such as flow cytometry and immunohistochemistry to establish their characteristics and compare them across different sources.
  • Further, immunohistochemistry was used on histological sections to understand the position and distribution of these cells within the tissue structure. During these investigations, several surface markers were utilized.

Findings

  • This study indicated the successful isolation and cultivation of MSCs from equine dental pulp and PDL. However, the researchers stress on the fact that the extraction process needs careful execution and is complicated.
  • The majority of the cultivated cells showed a positive response for CD90 and CD44 (markers for MSCs), and a negative response for CD11a/18, CD45, CD105 and MHCII. These results suggest that these cells exhibit MSC characteristics.
  • The CD90 positive cells were predominant in the perivascular region and the subodontoblastic layer. This location information was determined using immunohistology of pulp and PDL sections.

Potential Applications

  • The findings show significant potential for innovative treatments in equine dental medicine.
  • Therapeutic possibilities include regeneration of periodontal lesions, improved periodontal re-attachment after dental replantation and stimulation of pulp-obliteration and apexification allied with endodontic therapies.

Cite This Article

APA
Heilen LB, Roßgardt J, Dern-Wieloch J, Vogelsberg J, Staszyk C. (2023). Isolation and cultivation as well as in situ identification of MSCs from equine dental pulp and periodontal ligament. Front Vet Sci, 10, 1116671. https://doi.org/10.3389/fvets.2023.1116671

Publication

ISSN: 2297-1769
NlmUniqueID: 101666658
Country: Switzerland
Language: English
Volume: 10
Pages: 1116671

Researcher Affiliations

Heilen, Laura Beate
  • Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Giessen, Germany.
Roßgardt, Jessica
  • Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Giessen, Germany.
Dern-Wieloch, Jutta
  • Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Giessen, Germany.
Vogelsberg, Jörg
  • Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Giessen, Germany.
Staszyk, Carsten
  • Faculty of Veterinary Medicine, Institute of Veterinary Anatomy, Histology and Embryology, Justus-Liebig-University, Giessen, Germany.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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