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Veterinary research communications2011; 35(8); 487-499; doi: 10.1007/s11259-011-9492-8

Characterization of adipose-derived equine and canine mesenchymal stem cells after incubation in agarose-hydrogel.

Abstract: Adult stem cells are of particular interest for the therapeutic approach in the field of regenerative medicine. Due to their ease of harvest, adipose-derived mesenchymal stem cells (ASCs) are an attractive stem cell source that has become increasingly popular. Critical aspects of applied cell therapies are the circumstances of transport from the laboratory towards the site of operation and cell delivery into the desired area. With regard to these issues, agarose-hydrogel was analyzed as a cell carrier matrix of equine and canine ASCs in vitro, which can be used for minimally invasive application. Isolated ASCs were expanded and 2.5 × 10(6) cells were combined with agarose-hydrogel to build a 0.4% hydrogel-cell solution which was stored at two temperatures (room temperature (RT) vs. 37 °C). Cell viability was investigated (live-dead assay) at different time points (0, 1, 6 and 24 h) in order to determine i) the effect of different temperatures on the cell survival as well as ii) the maximum possible time span before implantation. CFU-assay and WST-1 assay were performed after 24 h incubation in agarose-hydrogel and the cells were induced into adipogenic and osteogenic differentiation to analyze the effects of the incubation on the cell behaviour. No negative effect of the agarose-hydrogel incubation was determined on the different species' cell behaviour at either RT or 37 °C with any of the assays used. We can recommend agarose-hydrogel as a cell carrier for cell implantation with a storage period of up to 24 h at room temperature or at 37 °C prior to implantation.
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Publication Date: 2011-07-15 PubMed ID: 21755422DOI: 10.1007/s11259-011-9492-8Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research investigates the viability of adipose-derived (fat-tissue based) equine and canine stem cells when stored in an agarose-hydrogel solution before cell-therapy based medical treatment. The main findings suggest that these stem cells can be safely stored in this solution for up to 24 hours without negatively affecting their behavior.

Overview of the Study

  • This study was conducted to assess the efficiency of agarose-hydrogel as a cell carrier for adipose-derived mesenchymal stem cells (ASCs). These types of stem cells are often used in regenerative medicine due to their easy harvesting procedure.
  • The researchers focused on the delivery and transportation of these cells from the lab to the operation site, which can often affect their viability and effectiveness.
  • Agarose-hydrogel was selected as a medium to carry these cells as it enables minimally invasive application.

Methodology and Execution

  • ASCs were isolated, expanded and combined with an agarose-hydrogel to create a 0.4% hydrogel-cell solution.
  • This solution was then stored at two different temperatures, room temperature (RT) and 37 °C, to measure the impact of temperature on cell survival.
  • A live-dead assay was used to determine cell viability at different time points (0, 1, 6 and 24 hours). This was done to understand the maximum length of time cells can be stored before implantation.
  • Other assays like the CFU-assay and WST-1 assay were also performed after a 24-hour incubation period in the agarose-hydrogel to study its effects on cell behaviour. Additionally, the cells were induced to differentiate into fat and bone cells to further study the impact of the agarose-hydrogel incubation.

Key Findings and Implications

  • From the different assays and tests conducted, the researchers found that the agarose-hydrogel incubation had no adverse effects on either equine or canine stem cells at both tested temperatures.
  • Therefore, the study concludes that agarose-hydrogel could be an effective cell carrier medium for stem cells with a safe storage period of up to 24 hours at both room temperature and 37 °C before implantation.

Cite This Article

APA
Schwarz C, Leicht U, Drosse I, Ulrich V, Luibl V, Schieker M, Röcken M. (2011). Characterization of adipose-derived equine and canine mesenchymal stem cells after incubation in agarose-hydrogel. Vet Res Commun, 35(8), 487-499. https://doi.org/10.1007/s11259-011-9492-8

Publication

Veterinary research communications
ISSN: 1573-7446
NlmUniqueID: 8100520
Country: Switzerland
Language: English
Volume: 35
Issue: 8
Pages: 487-499

Researcher Affiliations

Schwarz, Christina
  • Laboratory for Experimental Surgery and Regenerative Medicine, Department of Surgery, Ludwig Maximilians University, Nussbaumstr. 20, 80336, Munich, Germany.
Leicht, Uta
    Drosse, Inga
      Ulrich, Veronika
        Luibl, Volker
          Schieker, Matthias
            Röcken, Michael

              MeSH Terms

              • Adipose Tissue / cytology
              • Animals
              • Cell Culture Techniques
              • Cell Differentiation
              • Dogs
              • Female
              • Horses
              • Hydrogels / chemistry
              • Male
              • Mesenchymal Stem Cells / cytology
              • Mesenchymal Stem Cells / physiology
              • Sepharose / chemistry

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