Omneity® Pellets
All-In-One Vitamin & Mineral Pellet
Characterization of equine adipose tissue-derived progenitor cells before and after cryopreservation.
Abstract: In horses, stem cell therapies are a promising tool to the treatment of many injuries, which are common consequences of athletic endeavor, resulting in high morbidity and often compromising the performance. In spite of many advantages, the isolation of stem cells similar to human, from equine adipose tissue, occurred only recently. The aim of this study was to isolate equine adipose tissue-derived progenitor cells (eAT-PC), to characterize their proliferative potential, and to study their differentiation capacity before and after cryopreservation. The cells, isolated from horse adipose tissue, presented similar fibroblast-like cell morphology in vitro. Their proliferation rate was evaluated during 63 days (23 passages) before and after cryopreservation. After the induction of osteogenic differentiation, von Kossa staining and positive immunostaining studies revealed the formation of calcified extracellular matrix confirming the osteogenic potential of these cells. Adipogenic differentiation was induced using two protocols: routine and other one developed by us, while our protocol requires a shorter time (Oil Red O staining revealed significant accumulation of lipid droplets after 7 days). Chondrogenic differentiation was observed after 21 days of induced pellet culture, as evidenced by histological (toluidine blue) and immunohistochemistry studies. Our data demonstrate that eAT-PC can be easily isolated and successfully expanded in vitro while presenting significant proliferating rate. These cells can be maintained undifferentiated in vitro and can efficiently undergo differentiation at least into mesodermal derivates. These eAT-PC properties were preserved even after cryopreservation. Our findings classify eAT-PC as a promising type of progenitor cells that can be applied in different cell therapies in equines.
Publication Date: 2009-02-07 PubMed ID: 19196122DOI: 10.1089/ten.tec.2008.0186Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The researchers have analyzed equine adipose tissue-derived progenitor cells, or eAT-PCs, examining their ability to proliferate and differentiate before and after cryopreservation. The study findings assert that these eAT-PCs have potential for use in different cell therapies for horse injuries.
Characteristics of Isolated Cells
- The eAT-PCs were isolated from horse adipose tissue, which is a type of fat tissue.
- In an in vitro environment, the cells demonstrated a similar morphology to fibroblast cells.
Proliferation Rate
- The researchers cultivated these isolated cells over a period of 63 days, covering 23 passages, to measure their proliferative potential.
- They observed similar proliferation rates before and after cryopreservation, substantiating the robustness of these cells, even after preservation in ultra-low temperatures.
Differentiation Potential
- To evaluate their differentiation capability, the isolated cells were subjected to osteogenic, adipogenic, and chondrogenic differentiation conditions.
- After osteogenic induction, successful formation of a calcified extracellular matrix was observed, illustrating the cells’ potential to form bone tissue.
- For adipogenic differentiation, two different methods were used, with the latest one developed by the researchers that reduces the differentiation time to 7 days. The appearance of significant lipid accumulation confirmed the cells’ ability to transform into adipose (fat) tissue.
- The cells were encouraged to differentiate into chondrogenic tissue (cartilage), with successful differentiation observed after 21 days.
Cryopreservation Impact
- Notably, all these characteristics of the cells, including their ability to differentiate into multiple types of cells, remained constructive even after cryopreservation.
- This aspect demonstrates the potential these cells have for long-term storage and their subsequent application in different cell therapies.
Conclusion
- Researchers concluded that these eAT-PCs from horses present a novel promising type of progenitor cells for various horse injury therapies.
- Their successful isolation, high proliferation rate, multi-differentiation potential, and their ability to preserve these properties post-cryopreservation make them a valuable resource for stem cell-based therapeutic interventions in equines.
Cite This Article
APA
Mambelli LI, Santos EJ, Frazão PJ, Chaparro MB, Kerkis A, Zoppa AL, Kerkis I.
(2009).
Characterization of equine adipose tissue-derived progenitor cells before and after cryopreservation.
Tissue Eng Part C Methods, 15(1), 87-94.
https://doi.org/10.1089/ten.tec.2008.0186 Publication
Researcher Affiliations
- Laboratory of Genetics, Butantan Institute, Sao Paulo, Brazil.
MeSH Terms
- Adipogenesis
- Adipose Tissue / cytology
- Animals
- Cell Proliferation
- Cell Separation
- Cell Shape
- Chondrogenesis
- Cryopreservation
- Horses
- Osteogenesis
- Stem Cells / cytology
Citations
This article has been cited 14 times.- Petrova V, Yonkova P, Simeonova G, Vachkova E. Horse serum potentiates cellular viability and improves indomethacin-induced adipogenesis in equine subcutaneous adipose-derived stem cells (ASCs). Int J Vet Sci Med 2023;11(1):94-105.
- Petrova V, Vachkova E. Outlook of Adipose-Derived Stem Cells: Challenges to Their Clinical Application in Horses. Vet Sci 2023 May 12;10(5).
- Duan W, Lopez MJ, Hicok K. Adult multipotent stromal cell cryopreservation: Pluses and pitfalls. Vet Surg 2018 Jan;47(1):19-29.
- Wang L, Huang C, Li Q, Xu X, Liu L, Huang K, Cai X, Xiao J. Osteogenic differentiation potential of adipose-derived stem cells from ovariectomized mice. Cell Prolif 2017 Apr;50(2).
- Bittencourt MK, Barros MA, Martins JF, Vasconcellos JP, Morais BP, Pompeia C, Bittencourt MD, Evangelho KD, Kerkis I, Wenceslau CV. Allogeneic Mesenchymal Stem Cell Transplantation in Dogs With Keratoconjunctivitis Sicca. Cell Med 2016 Dec 3;8(3):63-77.
- Arnhold S, Wenisch S. Adipose tissue derived mesenchymal stem cells for musculoskeletal repair in veterinary medicine. Am J Stem Cells 2015;4(1):1-12.
- Fu Y, Li R, Zhong J, Fu N, Wei X, Cun X, Deng S, Li G, Xie J, Cai X, Lin Y. Adipogenic differentiation potential of adipose-derived mesenchymal stem cells from ovariectomized mice. Cell Prolif 2014 Dec;47(6):604-14.
- Favaron PO, Mess A, Will SE, Maiorka PC, de Oliveira MF, Miglino MA. Yolk sac mesenchymal progenitor cells from New World mice (Necromys lasiurus) with multipotent differential potential. PLoS One 2014;9(2):e95575.
- Mambelli LI, Mattos RC, Winter GH, Madeiro DS, Morais BP, Malschitzky E, Miglino MA, Kerkis A, Kerkis I. Changes in expression pattern of selected endometrial proteins following mesenchymal stem cells infusion in mares with endometrosis. PLoS One 2014;9(6):e97889.
- Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential. Stem Cell Res Ther 2014 Feb 21;5(1):25.
- Ranera B, Remacha AR, Álvarez-Arguedas S, Romero A, Vázquez FJ, Zaragoza P, Martín-Burriel I, Rodellar C. Effect of hypoxia on equine mesenchymal stem cells derived from bone marrow and adipose tissue. BMC Vet Res 2012 Aug 22;8:142.
- Violini S, Gorni C, Pisani LF, Ramelli P, Caniatti M, Mariani P. Isolation and differentiation potential of an equine amnion-derived stromal cell line. Cytotechnology 2012 Jan;64(1):1-7.
- Raabe O, Shell K, Würtz A, Reich CM, Wenisch S, Arnhold S. Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells. Vet Res Commun 2011 Aug;35(6):355-65.
- Nascimento C, Saraiva MVA, Pereira VM, de Brito DCC, de Aguiar FLN, Alves BG, Roballo KCS, de Figueiredo JR, Ambrósio CE, Rodrigues APR. Addition of synthetic polymer in the freezing solution of mesenchymal stem cells from equine adipose tissue as a future perspective for reducing of DMSO concentration. Braz J Vet Med 2023;45:e002523.
Use Nutrition Calculator
Check if your horse's diet meets their nutrition requirements with our easy-to-use tool Check your horse's diet with our easy-to-use tool
Talk to a Nutritionist
Discuss your horse's feeding plan with our experts over a free phone consultation Discuss your horse's diet over a phone consultation
Submit Diet Evaluation
Get a customized feeding plan for your horse formulated by our equine nutritionists Get a custom feeding plan formulated by our nutritionists
