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Home / Equine Research Bank / J Virol / Characterization of equine infectious anemia virus long term...
Journal of virology1987; 61(3); 743-747; doi: 10.1128/JVI.61.3.743-747.1987

Characterization of equine infectious anemia virus long terminal repeat.

Abstract: The long terminal repeats (LTRs) of equine infectious anemia virus (EIAV) were examined with respect to their ability to function as transcriptional promoters in various cellular environments. Nucleotide sequence analyses of the LTRs derived from two unique proviral clones revealed the requisite consensus transcription and processing signals. One of the proviruses possessed a duplication of a 16-base-pair sequence in the CCAAT box region of the LTR which was absent in the other provirus. To assess its functional activity, each LTR was coupled to the bacterial chloramphenicol acetyltransferase gene and transfected onto various cell lines, including matched cultures of EIAV-infected and uninfected cells. The levels of chloramphenicol acetyltransferase activity directed by the EIAV LTRs were between 250 and 900 times greater in EIAV-infected cells compared with their uninfected counterparts. Thus, EIAV expression appears to be activated by a virus-induced trans-activation phenomenon analogous to that recently shown to amplify expression of certain other lentiviruses.
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Publication Date: 1987-03-01 PubMed ID: 3027401PubMed Central: PMC254015DOI: 10.1128/JVI.61.3.743-747.1987Google Scholar: Lookup
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Summary

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The research article examines the ability of the long terminal repeats (LTRs) of the Equine Infectious Anemia Virus (EIAV) to function as transcriptional promoters in different cellular environments. The study also finds that EIAV expression seems to be enhanced by a virus-induced trans-activation phenomenon similar to that shown in other lentiviruses.

Nucleotide Sequence Analysis

  • The researchers conducted a nucleotide sequence analysis of the LTRs sourced from two distinct proviral clones. The analysis showed essential consensus transcription and processing signals.
  • One provirus showed a duplication of a 16-base-pair sequence in the CCAAT box region of the LTR, which the other did not possess.

Transfection Assessments

  • In order to evaluate the functional activity of each LTR, they were each conjoined to the bacterial chloramphenicol acetyltransferase gene, and then transfected onto various cell lines.
  • This included paired cultures of EIAV infected, and non-infected cells.

Results of the Study

  • The levels of Chloramphenicol acetyltransferase activity directed by EIAV’s LTRs proved to be between 250 and 900 times increased in the EIAV infected cells compared to the non-infected cells.
  • These results lead to the conclusion that the EIAV expression seems to be activated by a virus-induced trans-activation phenomenon akin to that seen in other lentiviruses.

Cite This Article

APA
Derse D, Dorn PL, Levy L, Stephens RM, Rice NR, Casey JW. (1987). Characterization of equine infectious anemia virus long terminal repeat. J Virol, 61(3), 743-747. https://doi.org/10.1128/JVI.61.3.743-747.1987

Publication

Journal of virology
ISSN: 0022-538X
NlmUniqueID: 0113724
Country: United States
Language: English
Volume: 61
Issue: 3
Pages: 743-747

Researcher Affiliations

Derse, D
    Dorn, P L
      Levy, L
        Stephens, R M
          Rice, N R
            Casey, J W

              MeSH Terms

              • Base Sequence
              • Gene Expression Regulation
              • Infectious Anemia Virus, Equine / genetics
              • Promoter Regions, Genetic
              • RNA, Messenger / genetics
              • Repetitive Sequences, Nucleic Acid
              • Species Specificity
              • Transcription, Genetic

              Grant Funding

              • N01-CO-23910 / NCI NIH HHS

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