Characterization of Equine Parvovirus in Thoroughbred Breeding Horses from Germany.
Abstract: An equine parvovirus-hepatitis (EqPV-H) has been recently identified in association with equine serum hepatitis, also known as Theiler's disease. The disease was first described by Arnold Theiler in 1918 and is often observed with parenteral use of blood products in equines. However, natural ways of viral circulation and potential risk factors for transmission still remain unknown. In this study, we investigated the occurrence of EqPV-H infections in Thoroughbred horses in northern and western Germany and aimed to identify potential risk factors associated with viral infections. A total of 392 Thoroughbreds broodmares and stallions were evaluated cross-sectionally for the presence of anti-EqPV-H antibodies and EqPV-H DNA using a luciferase immunoprecipitation assay (LIPS) and a quantitative PCR, respectively. In addition, data regarding age, stud farm, breeding history, and international transportation history of each horse were collected and analysed. An occurrence of 7% EqPV-H DNA positive and 35% seropositive horses was observed in this study cohort. The systematic analysis of risk factors revealed that age, especially in the group of 11-15-year-old horses, and breeding history were potential risk factors that can influence the rate of EqPV-H infections. Subsequent phylogenetic analysis showed a high similarity on nucleotide level within the sequenced Thoroughbred samples. In conclusion, this study demonstrates circulating EqPV-H infections in Thoroughbred horses from central Europe and revealed age and breeding history as risk factors for EqPV-H infections.
Publication Date: 2019-10-18 PubMed ID: 31635426PubMed Central: PMC6833105DOI: 10.3390/v11100965Google Scholar: Lookup
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- Journal Article
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Summary
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This research article discusses a study which investigates an equine parvovirus-hepatitis (EqPV-H) found in certain Thoroughbred horses from Germany. The investigation utilized a method of antibody detection and DNA testing, and analyzed factors such as age, farm, breeding history, and transportation history to understand potential risk factors.
Objectives and Methodology
- The study aims to understand the occurrence of EqPV-H infections in Thoroughbred horses in northern and western Germany. The focus is also on identifying potential risk factors linked to viral infections.
- A total of 392 Thoroughbred broodmares and stallions are evaluated for the presence of anti-EqPV-H antibodies and EqPV-H DNA by using a luciferase immunoprecipitation assay (LIPS) and a quantitative PCR respectively.
- Data relating to age, stud farm, breeding history, and international transportation history of each horse is collected and analysed.
Findings
- The study discovered a 7% occurrence of EqPV-H DNA positive and 35% seropositive horses in the study cohort.
- Systematic analysis of the collected data revealed that age, particularly in the group of 11-15 year old horses, and breeding history were likely risk factors influencing the rate of EqPV-H infections.
- Further phylogenetic analysis exhibited a high similarity on the nucleotide level within the sequenced Thoroughbred samples.
Conclusion
- The study successfully demonstrates circulating EqPV-H infections in Thoroughbred horses from Central Europe.
- The study, additionally, highlights age and breeding history as risk factors for EqPV-H infections.
- This research could be instrumental in understanding and preventing the spread of this virus among horses, particularly those in breeding programs.
Cite This Article
APA
Meister TL, Tegtmeyer B, Brüggemann Y, Sieme H, Feige K, Todt D, Stang A, Cavalleri JV, Steinmann E.
(2019).
Characterization of Equine Parvovirus in Thoroughbred Breeding Horses from Germany.
Viruses, 11(10).
https://doi.org/10.3390/v11100965 Publication
Researcher Affiliations
- Department of Molecular and Medical Virology, Faculty of Medicine, Ruhr-University Bochum, 44801 Bochum, Germany. Toni.meister@rub.de.
- Institute for Experimental Virology, TWINCORE Centre for Experimental and Clinical Infection Research,a joint venture between the Medical School Hannover (MHH) and the Helmholtz Centre for InfectionResearch (HZI), 30625 Hannover, Germany. birthe.tegtmeyer@twincore.de.
- Department of Molecular and Medical Virology, Faculty of Medicine, Ruhr-University Bochum, 44801 Bochum, Germany. Yannick.brueggemann@rub.de.
- Institute of Virology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. Harald.sieme@tiho-hannover.de.
- Institute of Virology, University of Veterinary Medicine Hannover, 30559 Hannover, Germany. Karsten.feige@tiho-hannover.de.
- Department of Molecular and Medical Virology, Faculty of Medicine, Ruhr-University Bochum, 44801 Bochum, Germany. Daniel.todt@rub.de.
- Department of Molecular and Medical Virology, Faculty of Medicine, Ruhr-University Bochum, 44801 Bochum, Germany. Alexander.stang@rub.de.
- Department for Companion Animals and Horses, University of Veterinary Medicine, 1210 Vienna, Austria. Jessika.cavalleri@vetmeduni.ac.at.
- Department of Molecular and Medical Virology, Faculty of Medicine, Ruhr-University Bochum, 44801 Bochum, Germany. Eike.steinmann@rub.de.
MeSH Terms
- Age Factors
- Animals
- Antibodies, Viral / blood
- Breeding
- Female
- Germany / epidemiology
- Hepatitis, Viral, Animal / epidemiology
- Hepatitis, Viral, Animal / virology
- Horse Diseases / epidemiology
- Horse Diseases / virology
- Horses / virology
- Male
- Parvoviridae Infections / epidemiology
- Parvoviridae Infections / veterinary
- Parvovirus / classification
- Parvovirus / isolation & purification
- Phylogeny
- Prevalence
- Risk Factors
Conflict of Interest Statement
The authors declare no conflict of interest.
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