Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
Abstract: A set of five monoclonal antibodies (mAb) against porcine major histocompatibility complex (MHC), or swine leukocyte antigens (SLA), class II molecules has been characterized. These mAbs appear to recognize monomorphic determinants on SLA-DR (2F4, 1F12 and 2E9/13) and SLA-DQ (BL2H5 and BL4H2) molecules, as assessed by flow cytometry and immunoprecipitation. By Western blot, the 2F4, 1F12, BL2H5 and BL4H2 epitopes were located on the beta-chains of these molecules. mAbs 2F4 and 1F12 crossreact with leucocytes of dog, cattle, horse and human; mAbs 2E9/13, BL2H5 and BL4H2 bind leucocytes of cattle but not those of human, dog and horse. These mAbs effectively blocked the mixed lymphocyte reaction and the proliferative response to viral antigens (African swine fever virus) and to staphylococcal enterotoxin B. Therefore, these mAbs can be useful reagents for studying MHC class II molecules of pig and crossreactive species, and the immunological processes where they are involved.
Publication Date: 1997-05-01 PubMed ID: 9258612DOI: 10.1016/s0145-305x(97)00008-6Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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Researchers have characterized five monoclonal antibodies that are specific for the major histocompatibility antigens in pigs and have tested their crossreactivity with leukocytes of other domestic animals.
Characterization of Monoclonal Antibodies
- The researchers have developed and studied five monoclonal antibodies (mAb) that are specific to porcine major histocompatibility complex (MHC) class II molecules, also known as swine leukocyte antigens (SLA).
- The antibodies identified appear to recognize determinants on SLA-DR and SLA-DQ molecules, confirmed by flow cytometry and immunoprecipitation.
- The epitopes for the antibodies 2F4, 1F12, BL2H5, and BL4H2 were located on the beta-chains of these MHC class II molecules as determined by Western blot experiments.
Crossreactivity Studies
- The researchers tested the crossreactivity of these antibodies with leucocytes of various domestic species, including dogs, cattle, horses, and humans.
- The antibodies 2F4 and 1F12 crossreacted with leukocytes from all the tested species.
- However, mAbs 2E9/13, BL2H5, and BL4H2 only reacted with cattle leukocytes and not with those of human, dog, and horse.
Interference with Immunological Processes
- The study found that the monoclonal antibodies were successful in blocking mixed lymphocyte reaction and the proliferative response to viral antigens like the African swine fever virus and to staphylococcal enterotoxin B.
- This suggests a critical role for these antibodies in immune response regulation.
Implications and Applications
- These monoclonal antibodies can act as valuable tools for studying MHC class II molecules of pigs and the species that show cross-reactivity.
- They can also provide insights into the related immunological processes where these molecules are involved, which would be beneficial to a wide range of scientific fields, including immunology, veterinary medicine, and animal husbandry.
Cite This Article
APA
Bullido R, Doménech N, Alvarez B, Alonso F, Babín M, Ezquerra A, Ortuño E, Domínguez J.
(1997).
Characterization of five monoclonal antibodies specific for swine class II major histocompatibility antigens and crossreactivity studies with leukocytes of domestic animals.
Dev Comp Immunol, 21(3), 311-322.
https://doi.org/10.1016/s0145-305x(97)00008-6 Publication
Researcher Affiliations
- Centro de Investigación en Sanidad Animal, INIA, Madrid, Spain.
MeSH Terms
- Animals
- Antibodies, Monoclonal / biosynthesis
- Antibodies, Monoclonal / chemistry
- Antibody Specificity
- Cattle
- Cross Reactions
- Dogs
- Histocompatibility Antigens Class II / immunology
- Horses
- Humans
- Leukocytes / immunology
- Lymphocyte Activation
- Mice
- Mice, Inbred BALB C
- Organ Specificity / immunology
- Species Specificity
- Swine / immunology
- Transfection
Citations
This article has been cited 8 times.- Geervliet M, Lute LCP, Jansen CA, Rutten VPMG, Savelkoul HFJ, Tijhaar E. Differential immunomodulation of porcine bone marrow derived dendritic cells by E. coli Nissle 1917 and β-glucans.. PLoS One 2020;15(6):e0233773.
- Montoya M, Foni E, Solórzano A, Razzuoli E, Baratelli M, Bilato D, Córdoba L, Del Burgo MAM, Martinez J, Martinez-Orellana P, Chiapponi C, Perlin DS, Del Real G, Amadori M. Expression Dynamics of Innate Immunity in Influenza Virus-Infected Swine.. Front Vet Sci 2017;4:48.
- Moreno S, Alvarez B, Poderoso T, Revilla C, Ezquerra A, Alonso F, Dominguez J. Porcine monocyte subsets differ in the expression of CCR2 and in their responsiveness to CCL2.. Vet Res 2010 Sep-Oct;41(5):76.
- Rodríguez Pulido M, Sobrino F, Borrego B, Sáiz M. Attenuated foot-and-mouth disease virus RNA carrying a deletion in the 3' noncoding region can elicit immunity in swine.. J Virol 2009 Apr;83(8):3475-85.
- Chamorro S, Revilla C, Alvarez B, Alonso F, Ezquerra A, Domínguez J. Phenotypic and functional heterogeneity of porcine blood monocytes and its relation with maturation.. Immunology 2005 Jan;114(1):63-71.
- Chianini F, Majó N, Segalés J, Domínguez J, Domingo M. Immunohistological study of the immune system cells in paraffin-embedded tissues of conventional pigs.. Vet Immunol Immunopathol 2001 Oct;82(3-4):245-55.
- Blanco E, Garcia-Briones M, Sanz-Parra A, Gomes P, De Oliveira E, Valero ML, Andreu D, Ley V, Sobrino F. Identification of T-cell epitopes in nonstructural proteins of foot-and-mouth disease virus.. J Virol 2001 Apr;75(7):3164-74.
- Blanco E, McCullough K, Summerfield A, Fiorini J, Andreu D, Chiva C, Borrás E, Barnett P, Sobrino F. Interspecies major histocompatibility complex-restricted Th cell epitope on foot-and-mouth disease virus capsid protein VP4.. J Virol 2000 May;74(10):4902-7.
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