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BMC veterinary research2015; 11; 281; doi: 10.1186/s12917-015-0531-5

Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane.

Abstract: Isolation of mesenchymal stem cells (MSCs) in equines, has been reported for different tissues including bone marrow, adipose, umbilical cord, peripheral blood, and yolk sac. In regard to the MSCs derived from synovial fluid (SF) or membrane (SM), there is data available for humans, dogs, pigs, goats and horses. Especially in equines, these cells have being considered promising candidates for articular regeneration. Herein, we established and characterized MSCs obtained from equine SF and SM. Samples were obtained during arthroscopy and cultured using MEM (Minimum Essential Medium). MSCs were characterized by morphology and expression of specific markers for stem cells, pluripotency, inflammation, and cell cycle. Results: The medium MEM was more effective (97% ± 2) to maintain both cultures. The cultures were composed by adherent cells with fibroblast-like shape, which had a growth pattern represented by a sigmoidal curve. After the expansion, the cells were analyzed by flow cytometry for stem cells, inflammatory, and cell cycle markers, and both lineages showed significant expression of CD45, Oct3/4, Nanog, CD105, CD90, CD34, CD117, CD133, TRA-1-81, VEGF, and LY6a. In contrast, there were differences in the cell cycle phases between the lineages, which was not observed in relation to the mitochondrial electrical potential. Conclusions: Given the large impact that joint pathology has on the athletic performance horses, our results suggested that the SF and SM are promising sources of stem cells with satisfactory characteristics of growth and gene expression that can be used in equine regenerative medicine.
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Publication Date: 2015-11-10 PubMed ID: 26555093PubMed Central: PMC4640348DOI: 10.1186/s12917-015-0531-5Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article studies the characteristics of mesenchymal stem cells obtained from the synovial fluid and membrane of horses. The study indicates that these derived stem cells show promise for use in regenerative medicine, particularly for repairing joint damage in equines.

Objective

The main objective of the study was to establish and characterize mesenchymal stem cells (MSCs) derived from the equine synovial fluid (SF) and synovial membrane (SM). These cells were examined for their morphology and expression of specific markers related to stem cells, pluripotency, inflammation, and cell cycle.

Methodology

  • The researchers acquired samples during arthroscopy and cultured them using Minimum Essential Medium (MEM).
  • The cells were analyzed for their morphology and their growth pattern was observed.
  • Flow cytometry was used to analyse expression of certain markers in the cells. Markers are proteins that help identify and classify cells.

Results

  • The MEM medium was effective in maintaining the cultures with a 97% success rate. The cells were adherent with a fibroblast-like shape.
  • The growth pattern of the cells showed a sigmoidal curve, indicating a typical growth behaviour in biology where there’s an initial slow growth, followed by rapid growth and then a plateau.
  • Both lineages (from SF and SM) expressed notable levels of specific markers such as CD45, Oct3/4, Nanog, CD105, CD90, CD34, CD117, CD133, TRA-1-81, VEGF, and LY6a. These markers suggest the cells’ ability to proliferate and differentiate, confirming their stem cell characteristics.
  • However, there were differences in cell cycle phases between the lineages. This means that the cells from SF and SM differed in their growth and multiplication rate.
  • No differences were noted in the mitochondrial electrical potential, which indicates the cells’ energy production ability remained consistent, irrespective of their source.

Conclusions

The study concludes that the equine synovial fluid and synovial membrane are promising sources of stem cells. Given the significant impact joint pathologies have on horses’ athletic performance, these cells, characterized by satisfactory growth and gene expression, may be useful in equine regenerative medicine – specifically, for articular regeneration in equines suffering from joint problems.

Cite This Article

APA
Prado AA, Favaron PO, da Silva LC, Baccarin RY, Miglino MA, Maria DA. (2015). Characterization of mesenchymal stem cells derived from the equine synovial fluid and membrane. BMC Vet Res, 11, 281. https://doi.org/10.1186/s12917-015-0531-5

Publication

BMC veterinary research
ISSN: 1746-6148
NlmUniqueID: 101249759
Country: England
Language: English
Volume: 11
Pages: 281
PII: 281

Researcher Affiliations

Prado, Aline Ambrogi Franco
  • Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270, São Paulo, SP, Brazil. alineambrogifranco@usp.br.
Favaron, Phelipe Oliveira
  • Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270, São Paulo, SP, Brazil. phelipe.favaron@yahoo.com.br.
da Silva, Luis Claudio Lopes Correia
  • Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270, São Paulo, SP, Brazil. silvalc@usp.br.
Baccarin, Raquel Yvonne Arantes
  • Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270, São Paulo, SP, Brazil. baccarin@usp.br.
Miglino, Maria Angelica
  • Department of Surgery, School of Veterinary Medicine and Animal Science, University of Sao Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, Cidade Universitária, 05508-270, São Paulo, SP, Brazil. miglino@usp.br.
Maria, Durvanei Augusto
  • Laboratory of Biochemical and Biophisic, Butantan Institute, Av. Dr. Vital Brasil, 1500, 05503-900, Sao Paulo, Brazil. durvanei@usp.br.

MeSH Terms

  • Animals
  • Cell Cycle
  • Cell Proliferation
  • Cells, Cultured
  • Horses
  • Membrane Potential, Mitochondrial
  • Mesenchymal Stem Cells / cytology
  • Mesenchymal Stem Cells / physiology
  • Synovial Fluid / cytology
  • Synovial Membrane / cytology

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