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Home / Equine Research Bank / Animals (Basel) / Characterization of Myeloperoxidase in the Healthy Equine En...
Animals : an open access journal from MDPI2023; 13(3); 375; doi: 10.3390/ani13030375

Characterization of Myeloperoxidase in the Healthy Equine Endometrium.

Abstract: Myeloperoxidase (MPO), as a marker of neutrophil activation, has been associated with equine endometritis. However, in absence of inflammation, MPO is constantly detected in the uterine lumen of estrous mares. The aim of this study was to characterize MPO in the uterus of mares under physiological conditions as a first step to better understand the role of this enzyme in equine reproduction. Total and active MPO concentrations were determined, by ELISA and SIEFED assay, respectively, in low-volume lavages from mares in estrus ( = 26), diestrus ( = 18) and anestrus ( = 8) in absence of endometritis. Immunohistochemical analysis was performed on 21 endometrial biopsies randomly selected: estrus ( = 11), diestrus ( = 6) and anestrus ( = 4). MPO, although mostly enzymatically inactive, was present in highly variable concentrations in uterine lavages in all studied phases, with elevated concentrations in estrus and anestrus, while in diestrus, concentrations were much lower. Intracytoplasmic immunoexpression of MPO was detected in the endometrial epithelial cells, neutrophils and glandular secretions. Maximal expression was observed during estrus in mid and basal glands with a predominant intracytoplasmic apical reinforcement. In diestrus, immunopositive glands were sporadic. In anestrus, only the luminal epithelium showed residual MPO immunostaining. These results confirm a constant presence of MPO in the uterine lumen of mares in absence of inflammation, probably as part of the uterine mucosal immune system, and suggest that endometrial cells are a source of uterine MPO under physiological cyclic conditions.
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Publication Date: 2023-01-21 PubMed ID: 36766264PubMed Central: PMC9913682DOI: 10.3390/ani13030375Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This study focuses on the characterization of Myeloperoxidase (MPO) in the uterus of healthy mares, finding that MPO is always present in the uterine lumen, with variable concentrations depending on the reproductive phase. The research suggests that MPO may be part of the uterine mucosal immune system and come from endometrial cells under normal conditions.

Objective of the Research

  • The primary aim of this research was to understand and characterize the presence and role of Myeloperoxidase (MPO) in the uterus of healthy mares. Although MPO has previously been associated with equine endometritis, a type of inflammation, this study focuses on MPO’s consistent presence in the uterine lumen in the absence of inflammation.

Methodology and Findings

  • The researchers used different techniques (ELISA and SIEFED assay) to determine the total and active MPO concentrations in the uterine lavages obtained from mares during different reproductive phases: estrus, diestrus, and anestrus.
  • Immunohistochemical analysis was used to study the presence and pattern of MPO in endometrial biopsies randomly selected from mares in these phases.
  • The study found that although most MPO was enzymatically inactive, it was always present in variable, but sometimes high, concentrations in uterine lavages across all studied phases.
  • Intracytoplasmic immunoexpression of MPO was noted in endometrial epithelial cells, neutrophils, and glandular secretions, suggesting that these could be sources of uterine MPO.

Implications of the Findings

  • This study’s findings suggest that MPO could be part of the uterine mucosal immune system and serve a constant function in the uterus.
  • This research shines a light on the potential role of endometrial cells as a source of uterine MPO under normal physiological conditions.
  • The high expression of MPO during the estrus phase points to a possible role in reproduction, which merits further investigation.

Cite This Article

APA
Parrilla Hernández S, Franck T, Munaut C, Feyereisen É, Piret J, Farnir F, Reigner F, Barrière P, Deleuze S. (2023). Characterization of Myeloperoxidase in the Healthy Equine Endometrium. Animals (Basel), 13(3), 375. https://doi.org/10.3390/ani13030375

Publication

Animals : an open access journal from MDPI
ISSN: 2076-2615
NlmUniqueID: 101635614
Country: Switzerland
Language: English
Volume: 13
Issue: 3
PII: 375

Researcher Affiliations

Parrilla Hernández, Sonia
  • Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Franck, Thierry
  • Center for Oxygen Research and development (CORD), University of Liège, 4000 Liège, Belgium.
Munaut, Carine
  • Laboratory of Tumor and Developmental Biology, GIGA-Cancer, University of Liège, 4000 Liège, Belgium.
Feyereisen, Émilie
  • Laboratory of Tumor and Developmental Biology, GIGA-Cancer, University of Liège, 4000 Liège, Belgium.
Piret, Joëlle
  • Department of Morphology and Pathology, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Farnir, Frédéric
  • Biostatistics and Bioinformatics Applied to Veterinary Sciences, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Reigner, Fabrice
  • UE1297 PAO, INRAE, 37380 Nouzilly, France.
Barrière, Philippe
  • UE1297 PAO, INRAE, 37380 Nouzilly, France.
Deleuze, Stéfan
  • Physiology of Reproduction, Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
  • Equine and Companion Animal Reproduction, Veterinary Medicine Faculty, University of Liège, 4000 Liège, Belgium.

Grant Funding

  • (Fonds Spu00e9ciaux pour la Recherche) / This research was funded by F.S.R (Fonds Spu00e9ciaux pour la Recherche), University of Liu00e8ge, Belgium

Conflict of Interest Statement

The authors declare no conflict of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

References

This article includes 80 references
  1. Mayadas TN, Cullere X, Lowell CA. The multifaceted functions of neutrophils.. Annu Rev Pathol 2014;9:181-218.
    doi: 10.1146/annurev-pathol-020712-164023pmc: PMC4277181pubmed: 24050624google scholar: lookup
  2. Klebanoff SJ. Myeloperoxidase: friend and foe.. J Leukoc Biol 2005 May;77(5):598-625.
    doi: 10.1189/jlb.1204697pubmed: 15689384google scholar: lookup
  3. Borregaard N, Cowland JB. Granules of the human neutrophilic polymorphonuclear leukocyte.. Blood 1997 May 15;89(10):3503-21.
    doi: 10.1182/blood.V89.10.3503pubmed: 9160655google scholar: lookup
  4. Brinkmann V, Reichard U, Goosmann C, Fauler B, Uhlemann Y, Weiss DS, Weinrauch Y, Zychlinsky A. Neutrophil extracellular traps kill bacteria.. Science 2004 Mar 5;303(5663):1532-5.
    doi: 10.1126/science.1092385pubmed: 15001782google scholar: lookup
  5. Khan AA, Alsahli MA, Rahmani AH. Myeloperoxidase as an Active Disease Biomarker: Recent Biochemical and Pathological Perspectives.. Med Sci (Basel) 2018 Apr 18;6(2).
    doi: 10.3390/medsci6020033pmc: PMC6024665pubmed: 29669993google scholar: lookup
  6. Friedrichs K, Baldus S, Klinke A. Fibrosis in Atrial Fibrillation - Role of Reactive Species and MPO.. Front Physiol 2012;3:214.
    doi: 10.3389/fphys.2012.00214pmc: PMC3379725pubmed: 22723783google scholar: lookup
  7. Rudolph V, Andrié RP, Rudolph TK, Friedrichs K, Klinke A, Hirsch-Hoffmann B, Schwoerer AP, Lau D, Fu X, Klingel K, Sydow K, Didié M, Seniuk A, von Leitner EC, Szoecs K, Schrickel JW, Treede H, Wenzel U, Lewalter T, Nickenig G, Zimmermann WH, Meinertz T, Böger RH, Reichenspurner H, Freeman BA, Eschenhagen T, Ehmke H, Hazen SL, Willems S, Baldus S. Myeloperoxidase acts as a profibrotic mediator of atrial fibrillation.. Nat Med 2010 Apr;16(4):470-4.
    doi: 10.1038/nm.2124pmc: PMC2880896pubmed: 20305660google scholar: lookup
  8. Pulli B, Ali M, Iwamoto Y, Zeller MW, Schob S, Linnoila JJ, Chen JW. Myeloperoxidase-Hepatocyte-Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis.. Antioxid Redox Signal 2015 Dec 1;23(16):1255-69.
    doi: 10.1089/ars.2014.6108pmc: PMC4677570pubmed: 26058518google scholar: lookup
  9. Austin GE, Zhao WG, Adjiri A, Lu JP. Control of myeloperoxidase gene expression in developing myeloid cells.. Leuk Res 1996 Oct;20(10):817-20.
    doi: 10.1016/S0145-2126(96)00032-Xpubmed: 8960106google scholar: lookup
  10. Green PS, Mendez AJ, Jacob JS, Crowley JR, Growdon W, Hyman BT, Heinecke JW. Neuronal expression of myeloperoxidase is increased in Alzheimer's disease.. J Neurochem 2004 Aug;90(3):724-33.
    doi: 10.1111/j.1471-4159.2004.02527.xpubmed: 15255951google scholar: lookup
  11. Martínez-Marcos P, Carvajal-Serna M, Lázaro-Gaspar S, Pérez-Pé R, Muiño-Blanco T, Cebrián-Pérez JA, Casao A. Presence of melatonin-catabolizing non-specific enzymes myeloperoxidase and indoleamine 2,3-dioxygenase in the ram reproductive tract.. Reprod Domest Anim 2019 Dec;54(12):1643-1650.
    doi: 10.1111/rda.13574pubmed: 31587393google scholar: lookup
  12. Roumeguère T, Delree P, Van Antwerpen P, Rorive S, Vanhamme L, de Ryhove Lde L, Serteyn D, Wespes E, Vanhaerverbeek M, Boudjeltia KZ. Intriguing location of myeloperoxidase in the prostate: a preliminary immunohistochemical study.. Prostate 2012 Apr;72(5):507-13.
    doi: 10.1002/pros.21452pubmed: 21739466google scholar: lookup
  13. Yang Z, Xiao K, Wang W, Tang J, Sun P-P, Peng K-M, Song H. The Effect of Visfatin on Inflammatory Reaction in Uterus of LPS-Induced Rats. Int. J. Morphol. 2015;33:194–203.
    doi: 10.4067/S0717-95022015000100031google scholar: lookup
  14. Haegens A, Vernooy JH, Heeringa P, Mossman BT, Wouters EF. Myeloperoxidase modulates lung epithelial responses to pro-inflammatory agents.. Eur Respir J 2008 Feb;31(2):252-60.
    doi: 10.1183/09031936.00029307pubmed: 18057061google scholar: lookup
  15. Yang JJ, Preston GA, Pendergraft WF, Segelmark M, Heeringa P, Hogan SL, Jennette JC, Falk RJ. Internalization of proteinase 3 is concomitant with endothelial cell apoptosis and internalization of myeloperoxidase with generation of intracellular oxidants.. Am J Pathol 2001 Feb;158(2):581-92.
    doi: 10.1016/S0002-9440(10)64000-Xpmc: PMC1850298pubmed: 11159195google scholar: lookup
  16. Jerke U, Rolle S, Purfürst B, Luft FC, Nauseef WM, Kettritz R. β2 integrin-mediated cell-cell contact transfers active myeloperoxidase from neutrophils to endothelial cells.. J Biol Chem 2013 May 3;288(18):12910-9.
    doi: 10.1074/jbc.M112.434613pmc: PMC3642334pubmed: 23532856google scholar: lookup
  17. La Rocca G, Di Stefano A, Eleuteri E, Anzalone R, Magno F, Corrao S, Loria T, Martorana A, Di Gangi C, Colombo M, Sansone F, Patanè F, Farina F, Rinaldi M, Cappello F, Giannuzzi P, Zummo G. Oxidative stress induces myeloperoxidase expression in endocardial endothelial cells from patients with chronic heart failure.. Basic Res Cardiol 2009 May;104(3):307-20.
    doi: 10.1007/s00395-008-0761-9pubmed: 19030913google scholar: lookup
  18. Art T, Franck T, Lekeux P, de Moffarts B, Couëtil L, Becker M, Kohnen S, Deby-Dupont G, Serteyn D. Myeloperoxidase concentration in bronchoalveolar lavage fluid from healthy horses and those with recurrent airway obstruction.. Can J Vet Res 2006 Oct;70(4):291-6.
    pmc: PMC1562538pubmed: 17042382
  19. Wauters J, Pille F, Martens A, Franck T, Serteyn D, Gasthuys F, Meyer E. Equine myeloperoxidase: a novel biomarker in synovial fluid for the diagnosis of infection.. Equine Vet J 2013 May;45(3):278-83.
    doi: 10.1111/j.2042-3306.2012.00682.xpubmed: 23145879google scholar: lookup
  20. Ponthier J, Franck T, Parrilla-Hernandez S, Niesten A, de la Rebiere G, Serteyn D, Deleuze S. Concentration, activity and biochemical characterization of myeloperoxidase in fresh and post-thaw equine semen and their implication on freezability.. Reprod Domest Anim 2014 Apr;49(2):285-91.
    doi: 10.1111/rda.12270pubmed: 24479950google scholar: lookup
  21. Art T, Franck T, Gangl M, Votion D, Kohnen S, Deby-Dupont G, Serteyn D. Plasma concentrations of myeloperoxidase in endurance and 3-day event horses after a competition.. Equine Vet J Suppl 2006 Aug;(36):298-302.
    doi: 10.1111/j.2042-3306.2006.tb05557.xpubmed: 17402436google scholar: lookup
  22. Grulke S, Franck T, Gangl M, Péters F, Salciccia A, Deby-Dupont G, Serteyn D. Myeloperoxidase assay in plasma and peritoneal fluid of horses with gastrointestinal disease.. Can J Vet Res 2008 Jan;72(1):37-42.
    pmc: PMC2117365pubmed: 18214160
  23. Storms N, Medina Torres C, Franck T, Sole Guitart A, de la Rebière G, Serteyn D. Presence of Myeloperoxidase in Lamellar Tissue of Horses Induced by an Euglycemic Hyperinsulinemic Clamp.. Front Vet Sci 2022;9:846835.
    doi: 10.3389/fvets.2022.846835pmc: PMC8962398pubmed: 35359667google scholar: lookup
  24. Parrilla-Hernandez S, Ponthier J, Franck TY, Serteyn DD, Deleuze SC. High concentrations of myeloperoxidase in the equine uterus as an indicator of endometritis.. Theriogenology 2014 Apr 15;81(7):936-40.
    doi: 10.1016/j.theriogenology.2014.01.011pubmed: 24565475google scholar: lookup
  25. Rebordão MR, Amaral A, Fernandes C, Silva E, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. Enzymes Present in Neutrophil Extracellular Traps May Stimulate the Fibrogenic PGF(2α) Pathway in the Mare Endometrium.. Animals (Basel) 2021 Sep 6;11(9).
    doi: 10.3390/ani11092615pmc: PMC8469524pubmed: 34573581google scholar: lookup
  26. Rebordão MR, Amaral A, Lukasik K, Szóstek-Mioduchowska A, Pinto-Bravo P, Galvão A, Skarzynski DJ, Ferreira-Dias G. Constituents of neutrophil extracellular traps induce in vitro collagen formation in mare endometrium.. Theriogenology 2018 Jun;113:8-18.
    doi: 10.1016/j.theriogenology.2018.02.001pubmed: 29452855google scholar: lookup
  27. Rebordão MR, Carneiro C, Alexandre-Pires G, Brito P, Pereira C, Nunes T, Galvão A, Leitão A, Vilela C, Ferreira-Dias G. Neutrophil extracellular traps formation by bacteria causing endometritis in the mare.. J Reprod Immunol 2014 Dec;106:41-9.
    doi: 10.1016/j.jri.2014.08.003pubmed: 25218891google scholar: lookup
  28. Nazhat SA, Kitahara G, Kozuka N, Mido S, Sadawy M, Ali HE, Osawa T. Associations of periparturient plasma biochemical parameters, endometrial leukocyte esterase and myeloperoxidase, and bacterial detection with clinical and subclinical endometritis in postpartum dairy cows.. J Vet Med Sci 2018 Feb 20;80(2):302-310.
    doi: 10.1292/jvms.17-0478pmc: PMC5836768pubmed: 29332864google scholar: lookup
  29. Amaral A, Fernandes C, Rebordão MR, Szóstek-Mioduchowska A, Lukasik K, Pinto-Bravo P, Telo da Gama L, Jan Skarzynski D, Ferreira-Dias G. Myeloperoxidase Inhibition Decreases the Expression of Collagen and Metallopeptidase in Mare Endometria under In Vitro Conditions.. Animals (Basel) 2021 Jan 16;11(1).
    doi: 10.3390/ani11010208pmc: PMC7830995pubmed: 33467081google scholar: lookup
  30. Chapman AL, Mocatta TJ, Shiva S, Seidel A, Chen B, Khalilova I, Paumann-Page ME, Jameson GN, Winterbourn CC, Kettle AJ. Ceruloplasmin is an endogenous inhibitor of myeloperoxidase.. J Biol Chem 2013 Mar 1;288(9):6465-77.
    doi: 10.1074/jbc.M112.418970pmc: PMC3585080pubmed: 23306200google scholar: lookup
  31. Segelmark M, Persson B, Hellmark T, Wieslander J. Binding and inhibition of myeloperoxidase (MPO): a major function of ceruloplasmin?. Clin Exp Immunol 1997 Apr;108(1):167-74.
    doi: 10.1046/j.1365-2249.1997.d01-992.xpmc: PMC1904617pubmed: 9097926google scholar: lookup
  32. Panzani D, Di Vita M, Lainé A-L, Guillaume D, Rota A, Tesi M, Vannozzi I, Camillo F. Corpus Luteum Vascularization and Progesterone Production in Autumn and Winter Cycles of the Mare: Relationship Between Ultrasonographic Characteristics of Corpora Lutea and Plasma Progesterone Concentration in the Last Cycles Before Anestrus. J. Equine Vet. Sci. 2017;56:35–39.
    doi: 10.1016/j.jevs.2017.05.001google scholar: lookup
  33. LeBlanc MM, Magsig J, Stromberg AJ. Use of a low-volume uterine flush for diagnosing endometritis in chronically infertile mares.. Theriogenology 2007 Aug;68(3):403-12.
    doi: 10.1016/j.theriogenology.2007.04.038pubmed: 17543379google scholar: lookup
  34. Kozdrowski R, Sikora M, Buczkowska J, Nowak M, Raś A, Dzięcioł M. Effects of cycle stage and sampling procedure on interpretation of endometrial cytology in mares.. Anim Reprod Sci 2015 Mar;154:56-62.
    doi: 10.1016/j.anireprosci.2015.01.009pubmed: 25660623google scholar: lookup
  35. Overbeck W, Witte TS, Heuwieser W. Comparison of three diagnostic methods to identify subclinical endometritis in mares.. Theriogenology 2011 Apr 15;75(7):1311-8.
    doi: 10.1016/j.theriogenology.2010.12.002pubmed: 21251703google scholar: lookup
  36. Kenney R M D P. Equine Endometrial Biopsy. In: Da M., editor. Current Therapy in Theriogenology. W.B Saunders Company; Philadelphia, PA, USA: 1986. pp. 723–729.
  37. Schoon H-A, Schoon D, Klug E. Endometrial biopsies as an ancillary aid in diagnosis and prognosis of subfertility in the mare. Pferdeheilkunde Equine Med. 1992;8:355–362.
    doi: 10.21836/PEM19920603google scholar: lookup
  38. Franck T, Grulke S, Deby-Dupont G, Deby C, Duvivier H, Peters F, Serteyn D. Development of an enzyme-linked immunosorbent assay for specific equine neutrophil myeloperoxidase measurement in blood.. J Vet Diagn Invest 2005 Sep;17(5):412-9.
    doi: 10.1177/104063870501700502pubmed: 16312231google scholar: lookup
  39. Franck T, Kohnen S, Deby-Dupont G, Grulke S, Deby C, Serteyn D. A specific method for measurement of equine active myeloperoxidase in biological samples and in in vitro tests.. J Vet Diagn Invest 2006 Jul;18(4):326-34.
    doi: 10.1177/104063870601800402pubmed: 16921870google scholar: lookup
  40. Hoffmann C, Bazer FW, Klug J, Aupperle H, Ellenberger C, Schoon HA. Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium Expression patterns for mares suffering from endometrosis.. Theriogenology 2009 Jan 15;71(2):264-74.
    doi: 10.1016/j.theriogenology.2008.07.008pubmed: 18762329google scholar: lookup
  41. Khalilova IS, Dickerhof N, Mocatta TJ, Bhagra CJ, McClean DR, Obinger C, Kettle AJ. A myeloperoxidase precursor, pro-myeloperoxidase, is present in human plasma and elevated in cardiovascular disease patients.. PLoS One 2018;13(3):e0192952.
    doi: 10.1371/journal.pone.0192952pmc: PMC5873943pubmed: 29590135google scholar: lookup
  42. Kenney R, Doig P. Equine endometrial biopsy. Curr. Ther. Ther. 1986;2:723–729.
  43. Hoffmann C, Bazer F W, Klug J, Aupperle H, Ellenberger C, Özgen S, Schoon D, Schoon H-A. Immunohistochemical and histochemical identification of proteins and carbohydrates in the equine endometrium: Reaction patterns in the cycling mare. Pferdeheilkunde Equine Med. 2009;25:212–219.
    doi: 10.21836/PEM20090303google scholar: lookup
  44. Schöniger S, Gräfe H, Richter F, Schoon HA. Expression of indoleamine 2,3-dioxygenase 1 as transcript and protein in the healthy and diseased equine endometrium.. Res Vet Sci 2018 Jun;118:278-287.
    doi: 10.1016/j.rvsc.2018.03.001pubmed: 29547726google scholar: lookup
  45. Schöniger S, Gräfe H, Schoon H-A. Beta-defensin is a component of the endometrial immune defence in the mare. Pferdeheilkunde Equine Med. 2013;29:335–346.
    doi: 10.21836/PEM20130307google scholar: lookup
  46. Schöniger S, Böttcher D, Theuß T, Schoon HA. Expression of Toll-like receptors 2, 4 and 6 in equine endometrial epithelial cells: A comparative in situ and in vitro study.. Res Vet Sci 2017 Jun;112:34-41.
    doi: 10.1016/j.rvsc.2017.01.004pubmed: 28119161google scholar: lookup
  47. Churg A, Anderson WA. Induction of endometrial peroxidase synthesis and secretion by estrogen and estrogen antagonist.. J Cell Biol 1974 Aug;62(2):449-59.
    doi: 10.1083/jcb.62.2.449pmc: PMC2109411pubmed: 4426915google scholar: lookup
  48. McRae AC. The blood-uterine lumen barrier and exchange between extracellular fluids.. J Reprod Fertil 1988 Mar;82(2):857-73.
    doi: 10.1530/jrf.0.0820857pubmed: 3283350google scholar: lookup
  49. Ceusters JD, Mouithys-Mickalad AA, Franck TJ, Derochette S, Vanderplasschen A, Deby-Dupont GP, Serteyn DA. Effect of myeloperoxidase and anoxia/reoxygenation on mitochondrial respiratory function of cultured primary equine skeletal myoblasts.. Mitochondrion 2013 Sep;13(5):410-6.
    doi: 10.1016/j.mito.2012.12.004pubmed: 23268199google scholar: lookup
  50. de Araujo TH, Okada SS, Ghosn EE, Taniwaki NN, Rodrigues MR, de Almeida SR, Mortara RA, Russo M, Campa A, Albuquerque RC. Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages.. Cell Immunol 2013 Jan;281(1):27-30.
    doi: 10.1016/j.cellimm.2013.01.002pubmed: 23434459google scholar: lookup
  51. Wira CR, Grant-Tschudy KS, Crane-Godreau MA. Epithelial cells in the female reproductive tract: a central role as sentinels of immune protection.. Am J Reprod Immunol 2005 Feb;53(2):65-76.
    doi: 10.1111/j.1600-0897.2004.00248.xpubmed: 15790340google scholar: lookup
  52. Kolm G, Klein D, Knapp E, Watanabe K, Walter I. Lactoferrin expression in the horse endometrium: relevance in persisting mating-induced endometritis.. Vet Immunol Immunopathol 2006 Nov 15;114(1-2):159-67.
    doi: 10.1016/j.vetimm.2006.08.005pubmed: 16973221google scholar: lookup
  53. Katila T, Lock TF, Hoffmann WE, Smith AR. Lysozyme, alkaline phosphatase and neutrophils in uterine secretions of mares with differing resistance to endometritis.. Theriogenology 1990 Mar;33(3):723-32.
    doi: 10.1016/0093-691X(90)90549-9pubmed: 16726768google scholar: lookup
  54. Shibata T, Sakamoto J, Osaka Y, Neyatani N, Fujita S, Oka Y, Takagi H, Mori H, Fujita H, Tanaka Y, Sasagawa T. Myeloperoxidase in blood neutrophils during normal and abnormal menstrual cycles in women of reproductive age.. Int J Lab Hematol 2017 Apr;39(2):169-174.
    doi: 10.1111/ijlh.12599pubmed: 28013526google scholar: lookup
  55. Békési G, Kakucs R, Varbiro S, Feher J, Pazmany T, Magyar Z, Sprintz D, Szekacs B. Induced myeloperoxidase activity and related superoxide inhibition during hormone replacement therapy.. BJOG 2001 May;108(5):474-81.
    doi: 10.1111/j.1471-0528.2001.00108.xpubmed: 11368132google scholar: lookup
  56. Houdeau E, Larauche M, Monnerie R, Bueno L, Fioramonti J. Uterine motor alterations and estrous cycle disturbances associated with colonic inflammation in the rat.. Am J Physiol Regul Integr Comp Physiol 2005 Mar;288(3):R630-7.
    doi: 10.1152/ajpregu.00482.2004pubmed: 15539608google scholar: lookup
  57. Baiza-Gutman LA, Flores-Sánchez MM, Díaz-Flores M, Hicks JJ. Presence of uterine peroxidase activity in the rat early pregnancy.. Int J Biochem Cell Biol 2000 Feb;32(2):255-62.
    doi: 10.1016/S1357-2725(99)00061-8pubmed: 10687959google scholar: lookup
  58. Cummings AM, Metcalf JL. Mechanisms of the stimulation of rat uterine peroxidase activity by methoxychlor.. Reprod Toxicol 1994 Nov-Dec;8(6):477-86.
    doi: 10.1016/0890-6238(94)90030-2pubmed: 7881199google scholar: lookup
  59. Farley DB, Ford SP, Rosazza JP. Increase in uterine peroxidase activity in the rat uterus during oestrogen hyperaemia.. J Reprod Fertil 1992 Jul;95(2):551-8.
    doi: 10.1530/jrf.0.0950551pubmed: 1518009google scholar: lookup
  60. Okada H, Nawa M, Kimura J. In vitro effect of estrogens on the peroxidase activity of human endometrium.. J Clin Endocrinol Metab 1985 Sep;61(3):404-7.
    doi: 10.1210/jcem-61-3-404pubmed: 2991317google scholar: lookup
  61. Batra SC, Iosif CS. Effect of estrogen treatment on the peroxidase activity and estrogen receptors in the female rabbit urogenital tissues.. J Urol 1992 Sep;148(3):935-8.
    doi: 10.1016/S0022-5347(17)36781-2pubmed: 1324993google scholar: lookup
  62. Grant-Tschudy KS, Wira CR. Effect of estradiol on mouse uterine epithelial cell transepithelial resistance (TER).. Am J Reprod Immunol 2004 Oct;52(4):252-62.
    doi: 10.1111/j.1600-0897.2004.00218.xpubmed: 15494046google scholar: lookup
  63. Lewis GS. Role of ovarian progesterone and potential role of prostaglandin F2alpha and prostaglandin E2 in modulating the uterine response to infectious bacteria in postpartum ewes.. J Anim Sci 2003 Jan;81(1):285-93.
    doi: 10.2527/2003.811285xpubmed: 12597400google scholar: lookup
  64. Watson ED, Stokes CR, Bourne FJ. Influence of administration of ovarian steroids on the function of neutrophils isolated from the blood and uterus of ovariectomized mares.. J Endocrinol 1987 Mar;112(3):443-8.
    doi: 10.1677/joe.0.1120443pubmed: 3559453google scholar: lookup
  65. Chadwick JW, Fine N, Khoury W, Tasevski N, Sun CX, Boroumand P, Klip A, Glogauer M. Tissue-specific murine neutrophil activation states in health and inflammation.. J Leukoc Biol 2021 Jul;110(1):187-195.
    doi: 10.1002/JLB.4AB1020-248RRRpubmed: 33145850google scholar: lookup
  66. Seals RC, Wulster-Radcliffe MC, Lewis GS. Modulation of the uterine response to infectious bacteria in postpartum ewes.. Am J Reprod Immunol 2002 Jan;47(1):57-63.
    doi: 10.1034/j.1600-0897.2002.1o062.xpubmed: 11883750google scholar: lookup
  67. Klebanoff SJ. Iodination of bacteria: a bactericidal mechanism.. J Exp Med 1967 Dec 1;126(6):1063-78.
    doi: 10.1084/jem.126.6.1063pmc: PMC2138423pubmed: 4964565google scholar: lookup
  68. Davies MJ, Hawkins CL. The Role of Myeloperoxidase in Biomolecule Modification, Chronic Inflammation, and Disease.. Antioxid Redox Signal 2020 May 1;32(13):957-981.
    doi: 10.1089/ars.2020.8030pubmed: 31989833google scholar: lookup
  69. Kargapolova Y, Geißen S, Zheng R, Baldus S, Winkels H, Adam M. The Enzymatic and Non-Enzymatic Function of Myeloperoxidase (MPO) in Inflammatory Communication.. Antioxidants (Basel) 2021 Apr 5;10(4).
    doi: 10.3390/antiox10040562pmc: PMC8066882pubmed: 33916434google scholar: lookup
  70. Reilas T, Rivera Del Alamo MM, Liepina E, Yeste M, Katila T. Effects on the equine endometrium of cervical occlusion after insemination.. Theriogenology 2016 Mar 1;85(4):617-24.
    doi: 10.1016/j.theriogenology.2015.09.053pubmed: 26586278google scholar: lookup
  71. Ricketts SW, Alonso S. The effect of age and parity on the development of equine chronic endometrial disease.. Equine Vet J 1991 May;23(3):189-92.
    doi: 10.1111/j.2042-3306.1991.tb02752.xpubmed: 1884699google scholar: lookup
  72. Bradley PP, Christensen RD, Rothstein G. Cellular and extracellular myeloperoxidase in pyogenic inflammation.. Blood 1982 Sep;60(3):618-22.
    doi: 10.1182/blood.V60.3.618.618pubmed: 6286012google scholar: lookup
  73. Edwards SW, Nurcombe HL, Hart CA. Oxidative inactivation of myeloperoxidase released from human neutrophils.. Biochem J 1987 Aug 1;245(3):925-8.
    doi: 10.1042/bj2450925pmc: PMC1148221pubmed: 2822034google scholar: lookup
  74. King CC, Jefferson MM, Thomas EL. Secretion and inactivation of myeloperoxidase by isolated neutrophils.. J Leukoc Biol 1997 Mar;61(3):293-302.
    doi: 10.1002/jlb.61.3.293pubmed: 9060452google scholar: lookup
  75. Klebanoff SJ. Effect of estrogens on the myeloperoxidase-mediated antimicrobial system.. Infect Immun 1979 Jul;25(1):153-6.
    doi: 10.1128/iai.25.1.153-156.1979pmc: PMC443568pubmed: 225268google scholar: lookup
  76. Galijasevic S, Abdulhamid I, Abu-Soud HM. Melatonin is a potent inhibitor for myeloperoxidase.. Biochemistry 2008 Feb 26;47(8):2668-77.
    doi: 10.1021/bi702016qpubmed: 18237195google scholar: lookup
  77. Galijasevic S, Abdulhamid I, Abu-Soud HM. Potential role of tryptophan and chloride in the inhibition of human myeloperoxidase.. Free Radic Biol Med 2008 Apr 15;44(8):1570-7.
    doi: 10.1016/j.freeradbiomed.2008.01.003pmc: PMC2861567pubmed: 18279680google scholar: lookup
  78. Klinke A, Nussbaum C, Kubala L, Friedrichs K, Rudolph TK, Rudolph V, Paust HJ, Schröder C, Benten D, Lau D, Szocs K, Furtmüller PG, Heeringa P, Sydow K, Duchstein HJ, Ehmke H, Schumacher U, Meinertz T, Sperandio M, Baldus S. Myeloperoxidase attracts neutrophils by physical forces.. Blood 2011 Jan 27;117(4):1350-8.
    doi: 10.1182/blood-2010-05-284513pubmed: 20980678google scholar: lookup
  79. Lefkowitz DL, Gelderman MP, Fuhrmann SR, Graham S, Starnes JD 3rd, Lefkowitz SS, Bollen A, Moguilevsky N. Neutrophilic myeloperoxidase-macrophage interactions perpetuate chronic inflammation associated with experimental arthritis.. Clin Immunol 1999 May;91(2):145-55.
    doi: 10.1006/clim.1999.4696pubmed: 10227806google scholar: lookup
  80. Lefkowitz DL, Roberts E, Grattendick K, Schwab C, Stuart R, Lincoln J, Allen RC, Moguilevsky N, Bollen A, Lefkowitz SS. The endothelium and cytokine secretion: the role of peroxidases as immunoregulators.. Cell Immunol 2000 May 25;202(1):23-30.
    doi: 10.1006/cimm.2000.1638pubmed: 10873303google scholar: lookup

Citations

This article has been cited 2 times.
  1. Aboelmaaty AM, Elgharieb AEA, El-Debaky HA, Alkhadrawy JMH, Abou-Ahmed MM, Ghallab AM. Fluctuations of antimüllerian hormone, ovarian follicular reserve, and antioxidant status throughout the estrous cycle in aged mares. Open Vet J 2024 Nov;14(11):3132-3143.
    doi: 10.5455/OVJ.2024.v14.i11.44pubmed: 39737015google scholar: lookup
  2. Alkhadrawy JMH, Aboelmaaty AM, Abou-Ahmed MM, Ghallab AM. Effect of hCG and prostaglandin on ovarian, luteal development, and hormonal changes in embryo donor mares during the hot summer months in subtropics. Open Vet J 2024 Aug;14(8):2057-2072.
    doi: 10.5455/OVJ.2024.v14.i8.35pubmed: 39308718google scholar: lookup
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