Characterization of respiratory dendritic cells from equine lung tissues.
Abstract: Dendritic cells (DCs) are professional antigen-presenting cells that have multiple subpopulations with different phenotypes and immune functions. Previous research demonstrated that DCs have strong potential for anti-viral defense in the host. However, viruses including alphaherpesvirinae have developed strategies to interfere with the function or maturation of DCs, causing immune dysfunction and avoidance of pathogen elimination. The goal of the present study was to isolate and characterize equine lung-derived DCs (L-DCs) for use in studies of respiratory viruses and compare their features with equine blood-derived DCs (B-DCs), which are currently used for these types of studies. Results: We found that L-DCs were morphologically similar to B-DCs. Overall, B-DCs demonstrated higher expression of CD86 and CD172α than L-DCs, but both cell types expressed high levels of MHC class II and CD44, as well as moderate amounts of CD163, CD204, and Bla36. In contrast, the endocytic activity of L-DCs was elevated compared to that of B-DCs. Finally, mononuclear cells isolated from lung (L-MCs), which are used as precursors for L-DCs, expressed more antigen-presenting cell-associated markers such as MHC class II and CD172α compared to their counterparts from blood. Conclusions: Our results indicate that L-DCs may be in an earlier differentiation stage compared to B-DCs. Concurrent with this observation, L-MCs possessed significantly more antigen-uptake capacity compared to their counterparts from blood. It is likely that L-DCs play an important role in antigen uptake and processing of respiratory pathogens and are major contributors to respiratory tract immunity and may be ideal tools for future in vitro or ex vivo studies.
Publication Date: 2017-11-06 PubMed ID: 29110660PubMed Central: PMC5674750DOI: 10.1186/s12917-017-1240-zGoogle Scholar: Lookup
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Summary
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The article discusses the isolation and characterization of dendritic cells from equine lung tissues, in order to compare and study their features and responses against respiratory viruses. It was found that lung-derived dendritic cells, although at an early differentiation stage, exhibit enhanced antigen uptake ability which suggests a principal role in respiratory tract immunity.
Research Goals and Methods
- The goal of the study was to isolate and characterize dendritic cells (DCs) originating from horse lung tissue and compare them to DCs from blood. These cells play a crucial role in processing and presenting antigens for the immune system.
- The derivation of DCs from lung tissues aimed to enable further studies of respiratory viruses and their impacts on the immune system.
- The methods involved using advanced lab techniques to isolate these cells from both lung tissue and blood cultures. They were then analyzed for differing phenotypes and immune responses.
Results of the Research
- Lung-derived dendritic cells (L-DCs) were morphologically found to be quite similar to blood-derived dendritic cells (B-DCs).
- B-DCs exhibited higher expression levels of certain immune markers (CD86 and CD172α) compared to L-DCs. However, both types of DCs notably expressed significant amounts of major histocompatibility complex (MHC) class II and CD44, as well as moderate amounts of CD163, CD204, and Bla36.
- The endocytic activity (the process of internalizing antigens or pathogens) of L-DCs was higher than that of B-DCs, denoting an enhanced antigen uptake capacity of L-DCs.
- Mononuclear cells isolated from lungs (L-MCs), the precursors for L-DCs, displayed more antigen-presenting cell-associated markers such as MHC class II and CD172α than their blood-derived counterparts.
Conclusions and Implications
- L-DCs might be in an early differentiation stage compared to B-DCs, as suggested by the differences in antigen-uptake capabilities.
- Given their enhanced antigen uptake and processing capabilities, L-DCs potentially play a major role in equine respiratory tract immunity.
- These findings suggest that L-DCs could be useful in future in vitro or ex vivo studies exploring respiratory pathogens and their interactions with the equine immune response.
Cite This Article
APA
Lee Y, Kiupel M, Soboll Hussey G.
(2017).
Characterization of respiratory dendritic cells from equine lung tissues.
BMC Vet Res, 13(1), 313.
https://doi.org/10.1186/s12917-017-1240-z Publication
Researcher Affiliations
- Department of Pathobiology & Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, 784 Wilson Rd, A13, East Lansing, MI, 48824, USA.
- Department of Pathobiology & Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, 784 Wilson Rd, A13, East Lansing, MI, 48824, USA.
- Department of Pathobiology & Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, 784 Wilson Rd, A13, East Lansing, MI, 48824, USA. husseygi@msu.edu.
MeSH Terms
- Animals
- Antigen-Presenting Cells / cytology
- Antigen-Presenting Cells / immunology
- Cell Differentiation
- Cells, Cultured
- Dendritic Cells / cytology
- Dendritic Cells / immunology
- Female
- Horses
- Leukocytes, Mononuclear / cytology
- Leukocytes, Mononuclear / immunology
- Lung / cytology
- Male
Conflict of Interest Statement
CONSENT FOR PUBLICATION: Not applicable. COMPETING INTERESTS: The authors declare that they have no competing interests. PUBLISHER’S NOTE: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
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Citations
This article has been cited 3 times.- Sage SE, Nicholson P, Peters LM, Leeb T, Jagannathan V, Gerber V. Single-cell gene expression analysis of cryopreserved equine bronchoalveolar cells. Front Immunol 2022;13:929922.
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