Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses.
Abstract: The aim of the present study was to investigate the genetic relatedness and the antimicrobial resistance profiles of a collection of Austrian Streptococcus pneumoniae isolates from companion animals and horses. A total of 12 non-repetitive isolates presumptively identified as S. pneumoniae were obtained during routinely diagnostic activities between March 2009 and January 2017. Results: Isolates were confirmed as S. pneumoniae by bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recA and the 16S rRNA genes. Isolates were further characterized by pneumolysin polymerase chain reaction (PCR) and genotyped by multilocus sequence typing (MLST). Antimicrobial susceptibility testing was performed and resistance genes were detected by specific PCR assays. All isolates were serotyped. Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were detected. Two isolates from twelve displayed a multidrug-resistance pheno- and genotype. Conclusions: This study represents the first comprehensive investigation on characteristics of S. pneumoniae isolates recovered from Austrian companion animals and horses. The obtained results indicate that common human sero- (23F) and sequence type (ST36) implicated in causing invasive pneumococcal disease (IPD) may circulate in dogs. Isolates obtained from other examined animals seem to be host-adapted.
Publication Date: 2017-11-14 PubMed ID: 29137652PubMed Central: PMC5686899DOI: 10.1186/s13028-017-0348-2Google Scholar: Lookup
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Summary
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This research article primarily investigates the genetic similarities and resistance to antimicrobials of Streptococcus pneumoniae isolates obtained from Austrian companion animals and horses. The findings suggest that the commonly occurring human bacterial strain causing invasive pneumococcal disease may also be found in dogs, while the strains found in other animals appear to be host-adapted.
Methodology
- A total of 12 unique isolates identified as S. pneumoniae were collected between March 2009 and January 2017.
- These isolates were confirmed as S. pneumoniae utilizing various testing methods such as bile solubility and optochin susceptibility testing, matrix-assisted laser desorption-ionization-time of flight (MALDI-TOF) mass spectrometry and sequence analysis of a part recA and the 16S rRNA genes.
- Further characterization of these isolates was done through pneumolysin polymerase chain reaction (PCR) and genotyping by multilocus sequence typing (MLST).
- Antimicrobials susceptibility was tested and relevant resistance genes were identified using specific PCR assays. All isolates were serotyped (identified by the specific variant of the bacteria).
Findings
- Four sequence types (ST) (ST36, ST3546, ST6934 and ST6937) and four serotypes (3, 19A, 19F and 23F) were identified amongst the isolates.
- Two of the twelve isolates exhibited a multidrug-resistance pheno- and genotype.
- It was surmised from the research that a common human sequence and serotype implicated in causing invasive pneumococcal disease (IPD) could potentially circulate in dogs.
- The isolates obtained from animals other than dogs seemed to be host-adapted, meaning these specific bacterial strains appear to have specifically adapted to their respective host species.
Conclusion
- This study is the first extensive investigation into the characteristics of S. pneumoniae isolates recovered from Austrian companion animals and horses.
- The results could provide valuable insights in understanding how the bacteria may spread and adapt between different species, which in turn can aid preventative and treatment measures against the disease.
Cite This Article
APA
Ginders M, Leschnik M, Künzel F, Kampner D, Mikula C, Steindl G, Eichhorn I, Feßler AT, Schwarz S, Spergser J, Loncaric I.
(2017).
Characterization of Streptococcus pneumoniae isolates from Austrian companion animals and horses.
Acta Vet Scand, 59(1), 79.
https://doi.org/10.1186/s13028-017-0348-2 Publication
Researcher Affiliations
- Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria.
- Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria.
- Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria.
- Department for Companion Animals and Horses, University of Veterinary Medicine Vienna, Vienna, Austria.
- National Reference Laboratory for Pneumococcus, Austrian Agency for Health and Food Safety, Graz, Austria.
- The Healthcare Company of Styria, Graz, Austria.
- Centre of Infection Medicine, Department of Veterinary Medicine, Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany.
- Centre of Infection Medicine, Department of Veterinary Medicine, Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany.
- Centre of Infection Medicine, Department of Veterinary Medicine, Institute of Microbiology and Epizootics, Freie Universität Berlin, Berlin, Germany.
- Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria.
- Department of Pathobiology, Institute of Microbiology, University of Veterinary Medicine Vienna, Vienna, Austria. igor.loncaric@vetmeduni.ac.at.
MeSH Terms
- Animals
- Anti-Bacterial Agents / pharmacology
- Austria
- Dogs
- Horses / microbiology
- Host-Pathogen Interactions
- Humans
- Microbial Sensitivity Tests
- Pets / microbiology
- RNA, Ribosomal, 16S / genetics
- Rec A Recombinases / genetics
- Streptococcus pneumoniae / drug effects
- Streptococcus pneumoniae / genetics
- Zoonoses / microbiology
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This article has been cited 2 times.- Hammond A, Halliday A, Thornton HV, Hay AD. Predisposing factors to acquisition of acute respiratory tract infections in the community: a systematic review and meta-analysis.. BMC Infect Dis 2021 Dec 14;21(1):1254.
- Zhao F, Yang N, Wang X, Mao R, Hao Y, Li Z, Wang X, Teng D, Fan H, Wang J. In vitro/vivo Mechanism of Action of MP1102 With Low/Nonresistance Against Streptococcus suis Type 2 Strain CVCC 3928.. Front Cell Infect Microbiol 2019;9:48.
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