Characterization of the NRAMP1 (SLC11A1) gene in the horse (Equus caballus L.).
Abstract: The complete coding cDNA sequence of the horse NRAMP1 (SLC11A1) gene was determined (GenBank accession number AF354445). The nucleotide sequence of the horse NRAMP1 gene is similar to sequences of this gene in other species. The gene contains 15 exons whose total length of 1,635 bp corresponds to 544 amino acids constituting the resulting putative protein. Hydrophobicity profile analysis of the deduced horse NRAMP1 gene product showed a nearly identical structure with the mouse NRAMP1 protein. The gene was found to be located on the short arm of ECA 6p12-13 by fluorescence in situ hybridization (FISH) analysis. Five allelic variants of the 5' untranslated region (UTR) were identified at the nucleotide sequence level. PCR-RFLP polymorphisms for NlaIII, TaqI, MspI and AciI were detected. Four out of five alleles could be detected using TaqI and MspI restriction enzymes. Their haplotype frequencies were different in four genetically distinct horse breeds.
Publication Date: 2002-10-03 PubMed ID: 12358853DOI: 10.1046/j.1365-2370.2002.00348.xGoogle Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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This study outlines the identification and analysis of the NRAMP1 (SLC11A1) gene in horses. Five variations of this gene were found, with their frequency varying across different horse breeds.
Characterization of the Horse NRAMP1 Gene
- The researchers carried out a comprehensive examination of the NRAMP1 (SLC11A1) gene in horses. This gene has an essential function in the immune response across numerous species.
- The complete coding cDNA sequence of the horse NRAMP1 gene was determined. This data is available in the GenBank database (accession number AF354445).
- Comparisons revealed that the sequence of the horse NRAMP1 gene is very similar to the sequences of this gene in other species, indicating its general predominance among different species.
Structural Details of the Horse NRAMP1 gene
- The NRAMP1 gene in horses is composed of 15 exons, resulting in a total length of 1,635 base pairs.
- This length corresponds to the production of a protein made up of 544 amino acids.
- The resulting protein’s hydrophobicity profile, or its tendency to interact or not interact with water, was almost identical to that of the mouse NRAMP1 protein. This again highlights the gene’s cross-species prevalence.
Localization and Allelic Variants of the Horse NRAMP1 Gene
- The research found that the NRAMP1 gene in horses is located on the short arm of chromosome ECA 6p12-13. This localization was determined using fluorescence in situ hybridization (FISH), a technique that uses fluorescent probes to visualize genetic material in cells.
- In analyzing the 5′ untranslated region (UTR) of the horse NRAMP1 gene, researchers identified five different allele variants. The 5′ UTR is a section of the gene that does not code for protein but has significant regulatory functions.
- These allelic variants were identified at the nucleotide sequence level – the most fundamental level of genetic variation.
Detection and Frequency of Alleles in Different Horse Breeds
- Polymorphisms for the four restriction enzymes NlaIII, TaqI, MspI and AciI were detected through the use of polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). This technique allows for the detection of genetic variations.
- Four of the five alleles were detectable by the TaqI and MspI restriction enzymes.
- The analysis found different frequencies of the allelic haplotypes across four genetically distinct breeds of horses. This variation implies that different breeds might have different genetic predispositions in relation to the NRAMP1 gene, which could be related to their varying immune responses.
Cite This Article
APA
Matiasovic J, Kubícková S, Musilová P, Rubes J, Horín P.
(2002).
Characterization of the NRAMP1 (SLC11A1) gene in the horse (Equus caballus L.).
Eur J Immunogenet, 29(5), 423-429.
https://doi.org/10.1046/j.1365-2370.2002.00348.x Publication
Researcher Affiliations
- Faculty of Veterinary Medicine, Institute of Genetics, Brno 612 42, Czech Republic.
MeSH Terms
- 5' Untranslated Regions / genetics
- Amino Acid Sequence
- Animals
- Base Sequence
- Cation Transport Proteins / genetics
- Horses
- Hydrophobic and Hydrophilic Interactions
- Molecular Sequence Data
- Physical Chromosome Mapping
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Sequence Analysis, DNA
Citations
This article has been cited 3 times.- Bayerova Z, Janova E, Matiasovic J, Orlando L, Horin P. Positive selection in the SLC11A1 gene in the family Equidae.. Immunogenetics 2016 May;68(5):353-64.
- Horín P, Smola J, Matiasovic J, Vyskocil M, Lukeszová L, Tomanová K, Králík P, Glasnák V, Schröffelová D, Knoll A, Sedlinská M, Krenková L, Jahn P. Polymorphisms in equine immune response genes and their associations with infections.. Mamm Genome 2004 Oct;15(10):843-50.
- Cohen ND, Harrington J, Gros P, Del Castro L, Meyer D, Martens RJ. Nramp1 deletion does not confer susceptibility to Rhodococcus equi infection in mice.. Immunogenetics 2004 Apr;56(1):65-7.
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