Characterization of the responses of equine digital veins and arteries to calcitonin gene-related peptide.
Abstract: To compare responses of equine digital arteries (EDAs) and veins (EDVs) to human-αcalcitonin gene-related peptide (hαCGRP), evaluate effect of the endothelium, and characterize receptors and sources of endogenous CGRP. Methods: Palmar digital vessels (5 to 9/experiment) from healthy adult horses killed at an abattoir. Methods: Vessel rings were mounted under tension in organ baths containing Krebs-Henseleit solution at 30 °C, with relaxation responses examined in vessels preconstricted with a thromboxane-mimetic (3 × 10(-8)M). Responses of endothelium-intact (+e) and -denuded (-e) EDAs and EDVs to hαCGRP C10(-10) to 3 × 10(-7)M) were compared. Following incubation with an hαCGRP receptor antagonist (hαCGRP(8-37); 1 μM), responses of EDA(-e) and EDV(-e) to hαCGRP (10(-7)M) were obtained. Responses of endothelium-intact and -denuded arteries and veins to hαCGRP (3 × 10(-7)M) or capsaicin (10(-5)M) were evaluated as well as responses of endothelium-intact and -denuded EDA and EDV to hαCGRP (10(-10) to 10(-6)M) after incubation with endothelin-1 (ET-1; 10(-12)M). Results: hαCGRP resulted in nonendothelium, concentration-dependent relaxation in EDAs and EDVs, with greater responses in EDAs. Treatment with hαCGRP(8-37) had minimal effect on responses to hαCGRP in either vessel type. Capsaicin induced relaxation in both vessel types. There were no differences between responses to hαCGRP for vessels pretreated with ET-1 or vehicle. Conclusions: Both hαCGRP and capsaicin induced digital vasodilation unaffected by a functional endothelium. This suggested that endogenous CGRP likely emanates from sensory-motor nerves and may contribute to digital vasodilation.
Publication Date: 2011-07-07 PubMed ID: 21728859DOI: 10.2460/ajvr.72.7.975Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article describes a study comparing how the veins and arteries in a horse’s leg (equine digital arteries and veins) react to a particular peptide, with consideration given to the role of the inner lining of the blood vessels (endothelium). Language isn’t used to confirm the source of an important peptide involved in blood vessel dilation.
Study Overview and Methodology
- This study focused on understanding the effect of human-alpha calcitonin gene-related peptide (hαCGRP), a substance that can affect the dilation and constriction of blood vessels, on equine digital arteries (EDAs) and veins (EDVs) – the blood vessels of a horse’s hoof.
- The research was carried out on palmar digital vessels taken from healthy adult horses. These vessels were experimentally constricted using a thromboxane-mimetic, a substance that mimics the thromboxane hormone and causes blood vessels to constrict.
- The responses of these blood vessel samples, with and without endothelium, to varying concentrations of hαCGRP were compared. Endothelium is the thin layer of cells that lines the interior surface of blood vessels.
- By using an hαCGRP receptor antagonist, a substance that blocks the receptors of hαCGRP, the researchers studied the response of endothelium-less EDAs and EDVs to a constant concentration of hαCGRP.
- In addition, the effect of hαCGRP and capsaicin, which also causes blood vessel dilation, was also analyzed in both endothelium-intact and -denuded arteries and veins.
- The effect on the blood vessels when they were pre-treated with endothelin-1 (ET-1), a molecule known to constrict blood vessels, was also investigated.
Results and Conclusion
- Results showed that hαCGRP prompted a non-endothelium-dependent, concentration-sensitive relaxation in both EDAs and EDVs, with higher responses in EDAs. This means that hαCGRP caused the blood vessels to open up and allow more blood flow, regardless of the presence of endothelium.
- The hαCGRP receptor antagonist minimally affected the vessels’ responses to hαCGRP.
- In addition, capsaicin was found to induce a dilation effect in both types of blood vessels.
- There was no significant response difference in the vessels when they were pre-treated with ET-1 or its absence.
- From the overall findings, it was concluded that both hαCGRP and capsaicin induced dilation in the horse’s leg vessels, and this dilation was not impacted by the state of the endothelium. This suggests that CGRP likely originates from sensory-motor nerves and could be responsible for the dilation of the digital (lower leg) blood vessels in a horse’s hoof.
Cite This Article
APA
Katz LM, Marr CM, Elliott J.
(2011).
Characterization of the responses of equine digital veins and arteries to calcitonin gene-related peptide.
Am J Vet Res, 72(7), 975-981.
https://doi.org/10.2460/ajvr.72.7.975 Publication
Researcher Affiliations
- University Veterinary Hospital, School of Agriculture, Food Science and Veterinary Medicine, University College Dublin, Belfield, Dublin 4, Ireland. lisa.katz@ucd.ie
MeSH Terms
- Animals
- Arteries / drug effects
- Arteries / physiology
- Calcitonin Gene-Related Peptide / antagonists & inhibitors
- Calcitonin Gene-Related Peptide / pharmacology
- Capsaicin / pharmacology
- Endothelium, Vascular / drug effects
- Endothelium, Vascular / physiology
- Female
- Forelimb / blood supply
- Glucose
- Horses / physiology
- Humans
- Male
- Muscle, Smooth, Vascular / drug effects
- Muscle, Smooth, Vascular / physiology
- Prostaglandin Endoperoxides, Synthetic / pharmacology
- Receptors, Calcitonin Gene-Related Peptide / drug effects
- Receptors, Calcitonin Gene-Related Peptide / physiology
- Tromethamine
- Vasodilation
- Veins / drug effects
- Veins / physiology
Citations
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