Chemical and immunochemical studies on pregnant mare serum gonadotropin.
Abstract: Highly purified pregnant mare serum gonadotropin (PMSG) can be prepared from crude commercial preparations of PMSG by chromatography on sulfoethyl-Sephadex C-50 and gel filtration on Sephadex G-100. The preparation was examined by disc electrophoresis and gel filtration and found to be of high purity. Amino acid analysis shows similarities to pituitary gonadotropins. The PMSG contains a high content of proline and cystine and low amounts of the aromatic amino acids. Phenylalanine is the major amino terminal amino acid. The carbohydrate content totals 45% of which 10% is the content of sialic acid. The PMSG is relatively stable in 8 M urea or 4 M guanidine, but inactivated by performic acid oxidation or treatment with neuraminidase.
Antiserum to PMSG was characterized by agar diffusion, immunoelectrophoresis, and quantitative precipitin reactions; it was specific for PMSG and did not cross react with human or ovine interstitial cell-stimulating hormone and follicle-stimulating hormone.
Publication Date: 1972-03-15 PubMed ID: 4622386DOI: 10.1016/0005-2795(72)90168-7Google Scholar: Lookup
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- Journal Article
Summary
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The research article discusses how highly purified pregnant mare serum gonadotropin (PMSG) is prepared and its chemical and immunochemical properties. The PMSG is examined to be of high purity and has similar amino acid structures like pituitary gonadotropins, with 45% carbohydrate content. An antiserum to PMSG created for the study shows specificity to PMSG and does not cross-react with hormones from other species.
Preparation of PMSG
- The researchers prepare a highly purified Pregnant Mare Serum Gonadotropin (PMSG) from raw commercial PMSG products.
- The purification process involves chromatography on sulfoethyl-Sephadex C-50 and gel filtration on Sephadex G-100.
- This purified PMSG is then evaluated using disc electrophoresis and gel filtration to confirm its high purity.
Chemical Analysis of PMSG
- The researchers perform an amino acid analysis on the purified PMSG. This revealed similarities to pituitary gonadotropins—hormones secreted by the pituitary gland that play a critical role in regulating reproductive health.
- The PMSG contained a high content of proline (a proteinogenic amino acid) and cystine (an oxidized dimer form of the cysteine), showing further similarities.
- The researchers note low amounts of aromatic amino acids, with phenylalanine — an essential amino acid — being the primary amino-terminal amino acid.
Carbohydrate Content and Stability of PMSG
- The carbohydrate content of the PMSG accounts for up to 45% of its makeup, with sialic acid constituting 10% of the total carbohydrate content.
- The researchers found that PMSG can retain stability even when exposed to 8 M urea or 4 M guanidine conditions. However, it gets inactivated by performic acid oxidation or treatment with neuraminidase (an enzyme).
Antiserum Characterization
- To further validate their findings, the researchers utilized antiserum against PMSG. This antiserum was characterized by agar diffusion, immunoelectrophoresis, and quantitative precipitin reactions.
- They observed that the antiserum was specific to PMSG, demonstrating no cross-reactivity with the human or ovine interstitial cell-stimulating hormone and follicle-stimulating hormone.
- This non-reactivity demonstrates the unique nature of the PMSG compared to other gonadotropin hormones.
Cite This Article
APA
Schams D, Papkoff H.
(1972).
Chemical and immunochemical studies on pregnant mare serum gonadotropin.
Biochim Biophys Acta, 263(1), 139-148.
https://doi.org/10.1016/0005-2795(72)90168-7 Publication
Researcher Affiliations
MeSH Terms
- Amino Acid Sequence
- Amino Acids / analysis
- Animals
- Carbohydrates / analysis
- Chromatography, Gel
- Chromatography, Ion Exchange
- Cross Reactions
- Drug Stability
- Electrophoresis, Disc
- Female
- Follicle Stimulating Hormone
- Formates
- Gonadotropins, Equine / analysis
- Gonadotropins, Equine / blood
- Gonadotropins, Equine / isolation & purification
- Guanidines
- Horses
- Immune Sera
- Immunodiffusion
- Immunoelectrophoresis
- Luteinizing Hormone
- Neuraminic Acids / analysis
- Neuraminidase
- Pregnancy
- Rabbits
- Rats
- Urea
Citations
This article has been cited 3 times.- Borş SI, Dascălu DL, Borş A, Fahmy HM, Kandil OM, Abdoon ASS. Intraovarian Injection of Reconstituted Lyophilized Growth-Promoting Factor Extracted from Horse Blood Platelets (L-GF(equina)) Increases Oocytes Recovery and In Vitro Embryo Production in Holstein Cows. Animals (Basel) 2022 Sep 29;12(19).
- Antczak DF, de Mestre AM, Wilsher S, Allen WR. The equine endometrial cup reaction: a fetomaternal signal of significance. Annu Rev Anim Biosci 2013 Jan;1:419-42.
- Lösle M, Lin CW, Beil-Wagner J, Aebi M, Buch T. Comparison of pregnant mare serum gonadotropin products with surprising differences in protein content. Sci Rep 2025 Feb 25;15(1):6824.
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