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Home / Equine Research Bank / PLoS One / Chemical composition and lipid profile of mare colostrum and...
PloS one2020; 15(9); e0238921; doi: 10.1371/journal.pone.0238921

Chemical composition and lipid profile of mare colostrum and milk of the quarter horse breed.

Abstract: The objective of this study was to characterize the chemical composition and lipid profile of colostrum and milk of purebred Quarter Horse mares. Thirty-four (34) purebred mares were selected, which were then separated into groups according to age, birth order and lactation stage. Colostrum samples were collected in the first six hours after delivery and milk samples from the 7th postpartum day, with intervals of 14 days until the end of lactation. The samples were refrigerated and sent to the Milk Laboratory of the University (Laboleite-UFRN), where they were analyzed for chemical composition. Colostrum was assessed by refractometry. The lipid profile was determined by gas chromatography through a separation of methyl esters. The data were tabulated and subjected to descriptive statistics and analysis of variance by the F-Test, and the groups were compared by the Tukey test using a significance level of 5%. There was high protein content and reduced lactose content for the colostrum of the Quarter Horse mares, differing from other breeds. The milk composition was not influenced by the mares' age. However, variations in the lactation stage and in the birth order of the Quarter Horse mares altered the milk's chemical composition. There is variation in the lipid composition of milk according to the lactation stage, without changing the characteristic profile of the mares' milk or diminishing the nutritional quality of the lipid fraction.
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Publication Date: 2020-09-14 PubMed ID: 32925944PubMed Central: PMC7489553DOI: 10.1371/journal.pone.0238921Google Scholar: Lookup
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  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article is about a scientific study conducted to understand the chemical makeup and lipid profile of the colostrum (initial form of milk produced by mammals after delivery) and regular milk of purebred Quarter Horse mares.

Structured Objective of the Study

  • The primary aim of the research was to better understand and describe the chemical composition and lipid profile of Quarter Horse mares’ colostrum and milk.

Experiment Structure and Approach

  • Researchers selected a sample of thirty-four (34) purebred Quarter Horse mares for the study.
  • These horses were then arranged into groups based upon several factors like age, the order of birth, and their respective phase of lactation.
  • Colostrum samples were collected within the initial six hours post-delivery, and regular milk samples were gathered beginning on the seventh day after delivery, at fourteen-day intervals until the lactation period ended.

Sample Analysis

  • These samples were kept refrigerated and dispatched to the Milk Laboratory of the University (Laboleite-UFRN) where they underwent analysis for their chemical makeup.
  • The colostrum composition was evaluated using refractometry, a method that measures the bending of light when it passes through a substance, providing insights into that substance’s composition and purity.
  • The lipid profile was obtained through gas chromatography, a method that allows for the separation and identification of chemical compounds within a sample.

Statistical Analysis

  • Data from the study was put through descriptive statistics, testing for variance using the F-Test, and comparisons between group variables were made using the Tukey statistical test with a significance level of 5%.

Study Findings

  • The research highlighted a higher protein content in the colostrum from Quarter Horse mares when compared to other breeds, with a lower lactose content.
  • The age of the horse didn’t significantly affect the composition of its milk; however, the stage of lactation and birth order did impact its chemical composition.
  • The research also showed that the lipid composition of the milk seems to vary according to the stage of lactation, but that doesn’t change the characteristic profile of the mares’ milk or compromise the nutritional quality of the lipid fraction.

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Cite This Article

APA
Barreto ÍMLG, Urbano SA, Oliveira CAA, Macêdo CS, Borba LHF, Chags BME, Rangel AHN. (2020). Chemical composition and lipid profile of mare colostrum and milk of the quarter horse breed. PLoS One, 15(9), e0238921. https://doi.org/10.1371/journal.pone.0238921

Publication

PloS one
ISSN: 1932-6203
NlmUniqueID: 101285081
Country: United States
Language: English
Volume: 15
Issue: 9
Pages: e0238921
PII: e0238921

Researcher Affiliations

Barreto, Ícaro M L G
  • Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Urbano, Stela A
  • Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Oliveira, Chiara A A
  • Federal University of Bahia, Salvador, BA, Brazil.
Macêdo, Cláudia S
  • Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Borba, Luiz H F
  • Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Chags, Bruna M E
  • Federal University of Rio Grande do Norte, Natal, RN, Brazil.
Rangel, Adriano H N
  • Federal University of Rio Grande do Norte, Natal, RN, Brazil.

MeSH Terms

  • Animals
  • Body Weight
  • Breeding
  • Chromatography, Gas
  • Colostrum / chemistry
  • Female
  • Horses
  • Lipidomics / methods
  • Lipids / analysis
  • Milk / chemistry
  • Nutritive Value
  • Pregnancy
  • Refractometry

Conflict of Interest Statement

The authors have declared that no competing interests exist.

References

This article includes 34 references
  1. Danków R, Wójtowski J, Pikul J, Niznikowski R, Cais-sokolínska D. Effect of lactation on the hygiene quality and some milk physicochemical traits of the Wielkopolska mares.. Arch Tierz 2006; 49:201–206.
  2. Malacarne M, Martuzzi F, Summer A, Mariani P. Protein and fat composition of mare’s milk: some nutritional remarks with reference to human and cow’s milk.. Int dairy J 2002; 12:869–77.
  3. Santos EM, Almeida FD, Vieira AA, Pinto LB, Corassa A, Pimentel RM, Silva VP, Galzerano L. Lactação em éguas da raça Mangalarga Marchador: produção e composição do leite e ganho de peso dos potros lactentes.. R Bras Zootec 2005; 34(2):627–634.
  4. Egito AS, Girardet JM, Miclo L, Mollé D, Humbert G, Gaillard JL. Susceptibility of equine k—and b—caseins to hydrolysis by chymosin.. Int Dairy J 2001;11:885–93.
  5. Salamón RV, Salamón S, Csapó-kiss Z, Csapó J. Composition of mare’s colostrum and milk I. Fat content, fatty-acid composition and vitamin contents.. Acta Univ. Sapientiae 2009; 2(1):119–131.
  6. Csapó J, Stefler J, Martin TG, Makray S, Csapó-Kiss Z. Composition of mare’s colostrum and milk. Fat content, fatty acid composition and vitamin content.. Int Dairy J 1995; 5(4): 393–402.
  7. Pecka E, Dobrzański Z, Zachwieja A, Szulc T, Czyż K. Studies of composition and major protein level in milk and colostrum of mares.. Anim Sci J 2012 Feb;83(2):162-8.
    pubmed: 22339698doi: 10.1111/j.1740-0929.2011.00930.xgoogle scholar: lookup
  8. Fessas D, Iametti S, Schiraldi A, Bonomi F. Thermal unfolding of monomeric and dimeric beta-lactoglobulins.. Eur J Biochem 2001 Oct;268(20):5439-48.
    pubmed: 11606207doi: 10.1046/j.0014-2956.2001.02484.xgoogle scholar: lookup
  9. Santos EM, Zanine AM. Lactação em éguas.. Rev Port Ciênc Vet 2006, 101:17–23.
  10. Pieszka M, Łuszczyński J, Szeptalin A. Comparison of mare’s milk composition of different breeds.. Nauka Przyr. Technol. 2011;5, 6, #112.
  11. Kramer JK, Blackadar CB, Zhou J. Evaluation of two GC columns (60-m SUPELCOWAX 10 and 100-m CP Sil 88) for analysis of milkfat with emphasis on CLA, 18:1, 18:2 and 18:3 isomers, and short- and long-chain FA.. Lipids 2002 Aug;37(8):823-35.
    pubmed: 12371755doi: 10.1007/s11745-002-0967-2google scholar: lookup
  12. Csapó J, Salamon S, Lóki K, Csapó-kiss Z. Composition of mare’s colostrum and milk II. Protein content, amino acid composition and contents of macro-and micro-elements.. Acta Univ Sapient Ser Aliment 2009; 2:133–148.
  13. Salimei E, Varisco G, Rosi F. Major constituents, leptin, and non-protein nitrogen compounds in mares' colostrum and milk.. Reprod Nutr Dev 2002 Jan-Feb;42(1):65-72.
    pubmed: 12199377doi: 10.1051/rnd:2002007google scholar: lookup
  14. Pikul J, Wójtowski J. Fat and cholesterol content and fatty acid composition of mares' colostrums and milk during five lactation months.. Livest Sci 2008; 113:285–290.
  15. Nath LC, Anderson GA, Savage CJ, McKinnon AO. Use of stored equine colostrum for the treatment of foals perceived to be at risk for failure of transfer of passive immunity.. J Am Vet Med Assoc 2010 May 15;236(10):1085-90.
    pubmed: 20470070doi: 10.2460/javma.236.10.1085google scholar: lookup
  16. Gibbs PG, Potter GD, Blake RW, McMullan WC. Milk production of quarter horse mares during 150 days of lactation.. J Anim Sci 1982 Mar;54(3):496-9.
    pubmed: 7085510doi: 10.2527/jas1982.543496xgoogle scholar: lookup
  17. Burns HD, Gibbs PG, Potter GD. Milk-energy production by lactating mares.. J Equine Vet Sci 1992; 12:118–120.
  18. Salimei S, Fantuz F. Equid milk for human consumption.. Int Dairy J 2012; 24:130–142.
  19. Santos EM, Almeida FD, Vieira AA, Pinto LB, Corassa A, Pimentel RM, Silva VP, Galzerano L. Lactação em éguas da raça Mangalarga Marchador: produção e composição do leite e ganho de peso dos potros lactentes.. R Bras Zootec 2005; 34(2):627–634.
  20. Reis ADP, Mesquita AJD, Moreira CHG, Curado EAF, Silva EBD, Nicolau ES. Composição do leite de éguas da raça Mangalarga Marchador.. Rev Inst Adolfo Lutz 2007; 66(2):130–135.
  21. Markiewicz-kęszycka M, Wójtowski J, Czyżak-runowska G, Kuczyńska B, Puppel K, Krzyżewski J, Bagnicka E. Concentration of selected fatty acids, fat-soluble vitamins and β-carotene in late lactation mares' milk.. Int dairy J 2014; 38:31–36.
  22. Doreau M, Martin-Rosset W. Horse.. Encyclopedia of Dairy Sciences 2002. Vol. 2.H.
  23. Bruckmaier RM. Normal and disturbed milk ejection in dairy cows.. Domest Anim Endocrinol 2005 Aug;29(2):268-73.
    pubmed: 15998500doi: 10.1016/j.domaniend.2005.02.023google scholar: lookup
  24. Ribeiro AB, Tinoco AFF, Lima GFC, Guilhermino MM, Rangel AHN. Produção e composição do leite de vacas Gir e Guzerá nas diferentes ordens de parto.. Rev. Caatinga 2008; 22:46–51.
  25. Rangel AHN, Braga AP, Aguiar EM, Lima Júnior DM, Lima RN. Fatores ambientais que afetam o desempenho produtivo de rebanhos da raça Jersey.. Rev Verde Agroecologia Desenvolv Sustent País 2008; 3:36–39.
  26. Pool-Anderson K, Raub RH, Warren JA. Maternal influences on growth and development of full-sibling foals.. J Anim Sci 1994 Jul;72(7):1661-6.
    pubmed: 7928744doi: 10.2527/1994.7271661xgoogle scholar: lookup
  27. Galvão Júnior JGB, Rangel AHN, Medeiros HR., Silva JBA, Aguiar EM, Madruga RC, De lima júnior DM. Efeito da produção diária e da ordem de parto na composição físico-química do leite de vacas de raças zebuínas.. Acta Vet Brasílica 2010; 4(1):25–30.
  28. Teodoro RL, Martinez ML, Verneque RS, Pires MFA. Parâmetros Genéticos e Fatores de Ajuste da Produção de Leite para o Efeito de Idade da Vaca ao Parto na Raça Guzerá.. Rev. bras. Zootec 2000; 29:2248–2252.
  29. Freitas MS, Durães MC, Freitas AF. Comparação da produção de leite e de gordura e da duração da lactação entre cinco “graus de sangue” originados de cruzamentos entre Holandês e Gir em Minas Gerais.. Arq Bras Med Vet Zootec 2001; 53:708–713.
  30. Pikul J, Wójtowski J, Danków R, Kuczyńska B, Lojek J. Fat content and fatty acids profile of colostrum and milk of primitive Konik horses ( Equus caballus gmelini Ant.) during six months of lactation.. J Dairy Res 2008 Aug;75(3):302-9.
    pubmed: 18680614doi: 10.1017/s0022029908003336google scholar: lookup
  31. Claeys WL, Verraes C, Cardoen S, De Block J, Huyghebaert A, Raes K, Dewettinck K, Herman L. Consumption of raw or heated milk from different species: An evaluation of the nutritional and potential health benefits.. Food Control 2014; 42:188–201.
  32. Orlandi M, Goracci J, Curadi MC. Fat composition of mare’s milk with reference to human nutrition.. Ann Fac Med Vet 2003; 56:97–105.
  33. Pieszka M, Łuszczyński J, Zamachowska M, Augustyn R, Długosz B, Hędrzak M. Is mare milk an appropriate food for people?–a review.. Ann Anim Sci 2016; 16(1):33–51.
  34. Markiewicz-kęszycka M, Wójtowski J, Czyżak-runowska G, Kuczyńska B, Puppel K, Krzyżewski J, Bagnicka E. Concentration of selected fatty acids, fat-soluble vitamins and β-carotene in late lactation mares' milk.. Int Dairy J 2014; 38:31–36.

Citations

This article has been cited 16 times.
  1. Barłowska J, Polak G, Janczarek I, Tkaczyk E. The Influence of Selected Factors on the Nutritional Value of the Milk of Cold-Blooded Mares: The Example of the Sokólski Breed. Animals (Basel) 2023 Mar 24;13(7).
    doi: 10.3390/ani13071152pubmed: 37048410google scholar: lookup
  2. Pyles MB, Brock K, Schendel RR, Lawrence LM. Improved methods for mare milk analysis: Extraction and quantification of mare milk carbohydrates and assessment of FTIR-based macronutrient quantification. Front Nutr 2023;10:1066463.
    doi: 10.3389/fnut.2023.1066463pubmed: 36742429google scholar: lookup
  3. Bigler NA, Bruckmaier RM, Gross JJ. Implications of placentation type on species-specific colostrum properties in mammals. J Anim Sci 2022 Dec 1;100(12).
    doi: 10.1093/jas/skac287pubmed: 36048628google scholar: lookup
  4. Gregić M, Mijić P, Baban M, Aladrović J, Pađen L, Gantner V, Bobić T. Changes in the Fatty Acid Composition of Milk of Lipizzaner Mares during the Lactation Period. Metabolites 2022 May 31;12(6).
    doi: 10.3390/metabo12060506pubmed: 35736439google scholar: lookup
  5. Czyżak-Runowska G, Wójtowski JA, Danków R, Stanisławski D. Mare's Milk from a Small Polish Specialized Farm-Basic Chemical Composition, Fatty Acid Profile, and Healthy Lipid Indices. Animals (Basel) 2021 May 28;11(6).
    doi: 10.3390/ani11061590pubmed: 34071465google scholar: lookup
  6. Boranbayeva T, Dossimova Z, Zhalelov D, Zhunisbek A, Bolat A, Toishimanov M. Influence of Lactation, Age and Foaling Factors on the Quality Composition, Fatty and Amino Acid Profile of Mare's Milk Under Pasture Conditions. Foods 2025 Aug 19;14(16).
    doi: 10.3390/foods14162880pubmed: 40870792google scholar: lookup
  7. Chakraborty A, Dutta P, Amrit R, Dey P, Osborne WJ. Antagonistic activity of butanamine 2,2-dinitro-N-methyl- synthesized by endosymbiotic Bacillus amyloliquefaciens VITAPRJS1 acquired from horse milk. Int Microbiol 2025 Oct;28(7):1583-1601.
    doi: 10.1007/s10123-025-00640-zpubmed: 39939508google scholar: lookup
  8. Gallacher K, Champion K, Denholm KS. Mare colostrum quality and relationship with foal serum immunoglobulin G concentrations and average daily weight gains. Equine Vet J 2025 Jul;57(4):904-914.
    doi: 10.1111/evj.14471pubmed: 39814454google scholar: lookup
  9. Rivero MJ, Cooke AS, Gandarillas M, Leon R, Merino VM, Velásquez A. Nutritional composition, fatty acids profile and immunoglobulin G concentrations of mare milk of the Chilean Corralero horse breed. PLoS One 2024;19(9):e0310693.
    doi: 10.1371/journal.pone.0310693pubmed: 39298497google scholar: lookup
  10. Wang L, Sun Y, Du L, Wang Q, Zhan M, Li S, Xiao X. Daily koumiss has positive regulatory effects on blood lipids and immune system: A metabolomics study. Heliyon 2024 Aug 30;10(16):e36429.
    doi: 10.1016/j.heliyon.2024.e36429pubmed: 39253138google scholar: lookup
  11. Ulaangerel T, Wang M, Zhao B, Yi M, Shen Y, Mengkh Y, Wen X, Dugarjav M, Bou G. A Comparative Analysis of the Gene Expression Profiles in the Mammary Glands of Lactating and Nonlactating Mares at the Second Month of Gestation. Animals (Basel) 2024 Aug 9;14(16).
    doi: 10.3390/ani14162319pubmed: 39199853google scholar: lookup
  12. Cao X, Fang Y, Bandan P, Suo L, Jiacuo G, Wu Y, Cuoji A, Zhuoga D, Chen Y, Ji D, Quzhen C, Zhang K. Age-specific composition of milk microbiota in Tibetan sheep and goats. Appl Microbiol Biotechnol 2024 Jul 9;108(1):411.
    doi: 10.1007/s00253-024-13252-wpubmed: 38980443google scholar: lookup
  13. Mönki J, Mykkänen A. Lipids in Equine Airway Inflammation: An Overview of Current Knowledge. Animals (Basel) 2024 Jun 18;14(12).
    doi: 10.3390/ani14121812pubmed: 38929431google scholar: lookup
  14. Martini M, Degl'Innocenti A, Altomonte I, Sodi I, Bocci C, Fanelli D, Moroni R, Panzani D, Camillo F, Salari F. Report on a Milking Mule: Milk Qualitative Characteristics during Lactation. Animals (Basel) 2024 May 27;14(11).
    doi: 10.3390/ani14111585pubmed: 38891633google scholar: lookup
  15. Witczak A, Mituniewicz-Małek A, Dmytrów I. Analysis of the Influence of Lactiplantibacillus plantarum and Lacticaseibacillus rhamnosus Strains on Changes in the Hexachlorobenzene Content in Fermented Mare Milk during Refrigerated Storage. Molecules 2024 Jan 21;29(2).
    doi: 10.3390/molecules29020528pubmed: 38276605google scholar: lookup
  16. Ren W, Sun M, Shi X, Wang T, Wang Y, Wang C, Li M. Progress of Mass Spectrometry-Based Lipidomics in the Dairy Field. Foods 2023 May 23;12(11).
    doi: 10.3390/foods12112098pubmed: 37297344google scholar: lookup
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