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Chronic exacerbation of equine heaves is associated with an increased expression of interleukin-17 mRNA in bronchoalveolar lavage cells.
Abstract: Recent finding suggests that T-cells may be involved in the pathogenesis of heaves in horses. However, little is known concerning their possible contribution to pulmonary neutrophilia, a characteristic finding in heaves. Interleukin (IL)-17 is a cytokine secreted by activated T-cells that indirectly promotes the maturation, chemotaxis and activation of neutrophils. We therefore hypothesized that IL-17 may be involved in the recruitment of neutrophils into the airways and that its mRNA expression would be increased in bronchoalveolar lavage (BAL) cells of horses with heaves. Heaves susceptible horses (n=4) and control horses (n=4) when in pasture (clinical remission) and after 35 days of continuous exposure to moldy hay were studied. BAL and respiratory mechanics measurements were performed at both time periods. The mRNA expression of IL-17 in BAL was studied using real-time polymerase chain reaction (PCR) and CD3-zeta was used as a marker of T-cell numbers. There was no significant difference in IL-17 mRNA expression between groups of horses while in pasture. However, stabling resulted in an increased expression of IL-17 in all horses with heaves but in none of the control horses. These preliminary results suggest that IL-17 may contribute in the pathogenesis of horses with heaves following chronic antigen challenge.
Publication Date: 2005-03-31 PubMed ID: 15797472DOI: 10.1016/j.vetimm.2004.12.013Google Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
- Asthma
- Bronchoalveolar Lavage
- Chronic Obstructive Pulmonary Disease
- Clinical Study
- Cytokines
- Diagnosis
- Disease Diagnosis
- Disease Etiology
- Equine Health
- Horses
- Immune Response
- Inflammatory Response
- Interleukins
- Neutrophils
- Pathogenesis
- Real-Time PCR
- Recurrent Airway Obstruction
- Respiratory Disease
- RNA
- T Cells
- Veterinary Medicine
Summary
This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.
The research article focuses on the investigation of a potential role of Interleukin (IL)-17, a type of protein, in the development of chronic respiratory condition known as ‘heaves’ in horses.
Overview of the Study
- This study was aimed at understanding the role of T-cells, which play a significant part in the immune response, in the pathogenesis or development of heaves in horses. Specifically, the researchers were interested in a cytokine (a type of protein that could influence immune response) called Interleukin-17, or IL-17, which is known to stimulate and enable neutrophils—white blood cells key to immune defense mechanisms.
- The scientists’ primary hypothesis was that IL-17 could be a significant factor in the recruitment of neutrophils to the airways, contributing to the development of heaves in horses. The study was specifically designed to measure and compare the expression of IL-17 in affected and unaffected horses.
Design and Findings of the Study
- Four horses susceptible to heaves and four control horses were examined when in pasture (during clinical remission), and after being exposed to moldy hay for 35 days, a known trigger for the condition.
- Measurements of respiratory mechanics and Bronchoalveolar Lavage (BAL)—a procedure which involves washing the bronchus and collecting the fluid for analysis to understand lung disease—were carried out during both periods of the study.
- The team used a real-time Polymerase Chain Reaction (PCR), an essential tool in medical and biological research to amplify and quantify DNA, to check the expression of mRNA (messenger RNA), which helps to produce proteins, for IL-17 in the BAL fluid. The amount of CD3-zeta, a marker for T-cell numbers, was also determined for comparison.
- No significant difference in IL-17 mRNA expression was noticed between the two groups while in pasture. However, exposure to moldy hay resulted in increased IL-17 mRNA expression in all heaves-prone horses, but not in any of the control horses.
Research Insights
- This suggests that an increase in IL-17 mRNA expression may contribute to the pathogenesis of heaves in horses that continuously encounter certain antigens or foreign substances, like moldy hay, that provoke immune responses. Consequently, IL-17 can potentially be a target for treatment strategies.
- The findings from the research are preliminary and offer a foundation for more extensive studies in the future to understand further and confirm IL-17’s role in the pathogenesis of heaves.
Cite This Article
APA
Debrue M, Hamilton E, Joubert P, Lajoie-Kadoch S, Lavoie JP.
(2005).
Chronic exacerbation of equine heaves is associated with an increased expression of interleukin-17 mRNA in bronchoalveolar lavage cells.
Vet Immunol Immunopathol, 105(1-2), 25-31.
https://doi.org/10.1016/j.vetimm.2004.12.013 Publication
Researcher Affiliations
- Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Montreal, 3200 Sicotte, Saint-Hyacinthe, Que., Canada J2S7C6.
MeSH Terms
- Airway Obstruction / genetics
- Airway Obstruction / immunology
- Airway Obstruction / veterinary
- Animals
- Bronchoalveolar Lavage / veterinary
- Bronchoalveolar Lavage Fluid / cytology
- Bronchoalveolar Lavage Fluid / immunology
- Female
- Gene Expression Regulation / immunology
- Horse Diseases / genetics
- Horse Diseases / immunology
- Horses
- Interleukin-17 / biosynthesis
- Interleukin-17 / genetics
- Interleukin-17 / immunology
- Lymphocyte Count / veterinary
- Neutrophils / immunology
- RNA, Messenger / biosynthesis
- RNA, Messenger / genetics
- Respiratory Function Tests / veterinary
- Reverse Transcriptase Polymerase Chain Reaction / veterinary
- Statistics, Nonparametric
Citations
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