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Home / Equine Research Bank / Front Physiol / Circulating miRNAs as Putative Biomarkers of Exercise Adapta...
Frontiers in physiology2018; 9; 429; doi: 10.3389/fphys.2018.00429

Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses.

Abstract: Endurance exercise induces metabolic adaptations and has recently been reported associated with the modulation of a particular class of small noncoding RNAs, microRNAs, that act as post-transcriptional regulators of gene expression. Released into body fluids, they termed circulating miRNAs, and they have been recognized as more effective and accurate biomarkers than classical serum markers. This study examined serum profile of miRNAs through massive parallel sequencing in response to prolonged endurance exercise in samples obtained from four competitive Arabian horses before and 2 h after the end of competition. MicroRNA identification, differential gene expression (DGE) analysis and a protein-protein interaction (PPI) network showing significantly enriched pathways of target gene clusters, were assessed and explored. Our results show modulation of more than 100 miRNAs probably arising from tissues involved in exercise responses and indicating the modulation of correlated processes as muscle remodeling, immune and inflammatory responses. Circulating miRNA high-throughput sequencing is a promising approach for sports medicine for the discovery of putative biomarkers for predicting risks related to prolonged activity and monitoring metabolic adaptations.
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Publication Date: 2018-04-24 PubMed ID: 29740341PubMed Central: PMC5928201DOI: 10.3389/fphys.2018.00429Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research highlights the potential role of circulating microRNAs as efficient biomarkers for exercise adaptation in endurance horses, where these RNAs release from tissues involved in exercise response and demonstrate the associated processes such as muscle remodeling and immune response.

Objective of the Study

  • The main aim of this investigation was to explore the alteration of serum microRNA profiles in response to strenuous endurance exercise in horses.
  • The study further intended to evaluate the potential of circulating microRNAs as reliable biomarkers of exercise adaptation that may potentially predict risks associated with extended physical activity and monitor metabolic changes.

Methodology and Analysis

  • The study took serum samples from four competitive Arabian horses both before and 2 hours after the end of competition. The researchers then performed massive parallel sequencing to evaluate the serum profiles of microRNAs.
  • They implemented MicroRNA identification, differential gene expression analysis, and constructed a protein-protein interaction network to investigate the enriched pathways of clustered target genes.

Findings of the Study

  • The researchers observed that endurance exercise modulates more than 100 different microRNAs, reflecting its possible origin from tissues involved in the exercise response such as muscles.
  • This modulation indicated related processes like muscle renovation, and immune and inflammatory responses influenced by the exercise.

Conclusions and Implications

  • Based on the results, the study concludes that high-throughput sequencing of circulating microRNAs present an innovative and promising approach in sports medicine.
  • This approach potentially aids in the discovery of putative biomarkers for predicting the risks related to extended physical exertion and for monitoring the metabolic changes and adaptations that occur in endurance horses.
  • However, as the study was based on a small sample size of four horses, further research with a larger sample might be required to corroborate these findings and to elucidate other potential impacts of endurance exercise.

Cite This Article

APA
Cappelli K, Capomaccio S, Viglino A, Silvestrelli M, Beccati F, Moscati L, Chiaradia E. (2018). Circulating miRNAs as Putative Biomarkers of Exercise Adaptation in Endurance Horses. Front Physiol, 9, 429. https://doi.org/10.3389/fphys.2018.00429

Publication

Frontiers in physiology
ISSN: 1664-042X
NlmUniqueID: 101549006
Country: Switzerland
Language: English
Volume: 9
Pages: 429
PII: 429

Researcher Affiliations

Cappelli, Katia
  • Dipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, Italy.
Capomaccio, Stefano
  • Dipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, Italy.
Viglino, Andrea
  • Facoltà di Scienze Agrarie, Alimentari e Ambientali, Istituto di Zootecnica, Università Cattolica del Sacro Cuore, Piacenza, Italy.
Silvestrelli, Maurizio
  • Dipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, Italy.
Beccati, Francesca
  • Dipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, Italy.
Moscati, Livia
  • Istituto Zooprofilattico Sperimentale dell'Umbria e delle Marche, Perugia, Italy.
Chiaradia, Elisabetta
  • Dipartimento di Medicina Veterinaria, Centro di Studio del Cavallo Sportivo, University of Perugia, Perugia, Italy.

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