CKM intron: an appropriate marker for the determination of the genetic relationships among horse populations and breeds.
Abstract: To date, the origins, domestication, and genetic structure of Chinese Mongolian horses (CMH) are poorly understood. Furthermore, there have been sparse reports on the genetic differences between CMH and Thoroughbred. In order to determine their genetic structure, understand their genetic relationships, and explore their domestication processes, we performed an extensive survey of creatine kinase (muscle isoenzyme; variations among six populations of indigenous CMH, cultivated Sanhe horses, and imported Thoroughbred. Twenty-three single-nucleotide polymorphisms were found among the 343 horse sequences. From these, 40 haplotypes were inferred. Haplotype diversity () values differed from 0.6424 to 0.7881 and nucleotide diversity () values ranged from 0.00150 to 0.00211. The differences between Thoroughbred population and other Chinese horse populations were large, but only small differences were observed among Chinese horse populations with respect to intron sequences suggesting that the domestication history, breeding measures, and origins of these horse populations are completely different. Results suggest that Sanhe and CMH are very closely related and the introgression (interbreeding) between them is serious. Our results suggest that Sanhe and Wushen require prompt and powerful protection. Overall, intron was an appropriate marker for the determination of genetic relationships among horse populations and breeds.
Publication Date: 2023-08-18 PubMed ID: 37593944DOI: 10.1080/10495398.2023.2247445Google Scholar: Lookup
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Summary
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This research focusses on understanding the genetic structure and relationships of Chinese Mongolian horses (CMH) and their domestication process, using a specific genetic marker called the creatine kinase, muscle isoenzyme (CKM) intron.
Research Purpose and Methodology
- The paper aims to shed light on the genetic structure, relationships, and origin of Chinese Mongolian horses (CMH), about which there is limited understanding.
- The research also attempts to clarify genetic differences between CMH and Thoroughbred horses.
- The study approach was to extensively analyze the variations in the creatine kinase, muscle isoenzyme (CKM) among indigenous CMH populations, cultivated Sanhe horses, and imported Thoroughbreds.
Key Findings
- The study identified 23 unique genetic variations (single-nucleotide polymorphisms) among the 343 horse sequences sampled.
- From these, 40 different genetic profiles (haplotypes) were determined.
- The research found a range of genetic diversity within the populations studied. Haplotype diversity varied between 0.6424 to 0.7881 and nucleotide diversity ranged between 0.00150 to 0.00211.
- There were large genetic differences revealed between Thoroughbred population and other Chinese horse populations, suggesting separate origins and distinct domestication/breeding histories.
Implications
- Small differences were observed among Chinese horse populations, indicating some commonality of CKM intron sequences. This suggests shared origins or interbreeding among these horse populations, especially between Sanhe and CMH.
- Sanhe and Wushen horse populations were identified as needing significant protection, due to their unique genetic heritage.
- The CKM intron was confirmed as an appropriate marker for determining genetic relationships among horse populations and breeds.
Cite This Article
APA
Chen J, Wang H, Li J, Liu S, Li B, Sun Y, Wang H, Manglai D.
(2023).
CKM intron: an appropriate marker for the determination of the genetic relationships among horse populations and breeds.
Anim Biotechnol, 1-9.
https://doi.org/10.1080/10495398.2023.2247445 Publication
Researcher Affiliations
- The Research Institute for the Development Strategy of the Equine Industry, Chifeng University, Chifeng, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
- The Research Institute for the Development Strategy of the Equine Industry, Chifeng University, Chifeng, China.
- College of Chemistry and Life Science, Chifeng University, Chifeng, China.
- The Research Institute for the Development Strategy of the Equine Industry, Chifeng University, Chifeng, China.
- College of Chemistry and Life Science, Chifeng University, Chifeng, China.
- Gene Bank for Equine Genetic Resources of Shandong Province, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
- Binzhou Refferral Center for Agricultural Technologies, Binzhou, China.
- Gene Bank for Equine Genetic Resources of Shandong Province, College of Animal Science and Technology, Qingdao Agricultural University, Qingdao, China.
- Vocational College of Dongying, Dongying, China.
- The Research Institute for the Development Strategy of the Equine Industry, Chifeng University, Chifeng, China.
- Inner Mongolia Key Laboratory of Equine Genetics, Breeding and Reproduction, College of Animal Science, Inner Mongolia Agricultural University, Hohhot, China.
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