Cleaning with a wet sterile gauze significantly reduces contamination of sutures, instruments, and surgical gloves in an ex-vivo pelvic flexure enterotomy model in horses.
Abstract: The objective of this study was to investigate whether cleaning surgical materials used to close pelvic flexure enterotomies with a wet sterile gauze will reduce contamination and whether the use of a full thickness appositional suture pattern (F) or a partial thickness inverting (or Cushing) suture pattern (C) would make a difference in the level of contamination. Large colon specimens were assigned to group F or C and divided into subgroups N and G. In group G, a wet sterile gauze was passed over the suture material, another over the instruments, and another over the gloves. In group N, no treatment was applied. The bacterial concentration was measured by optical density (OD) at 24 h. The OD of subgroup CG was lower than that of subgroup CN ( = 0.019). The OD of subgroup FG was lower than that of subgroup FN ( = 0.02). The OD of subgroups CG, CN, FG, and FN was lower than that of the negative control ( < 0.003, < 0.001, < 0.001, and < 0.00). The use of a sterile wet gauze significantly reduced contamination of suture materials. A partial thickness inverting suture pattern did not produce less contamination than a full thickness appositional suture pattern. L’objectif de la présente étude était d’examiner si le nettoyage du matériel chirurgical utilisé pour fermer les entérotomies de la courbure pelvienne avec une gaze stérile mouillée réduisait la contamination et si l’utilisation d’un patron de suture d’apposition de la pleine épaisseur (F) ou d’un patron de suture inversé d’une épaisseur partielle (ou Cushing) (C) faisait une différence dans le degré de contamination. Des spécimens du gros côlon ont été assignés au groupe F ou C dans les sous-groupes N et G. Dans le groupe G, une gaze stérile mouillée a été passée par-dessus le matériel de suture, une autre par-dessus les instruments, et une autre par-dessus les gants. Dans le groupe N, aucun traitement ne fut effectué. Les concentrations bactériennes ont été mesurées par densité optique (DO) à 24 h. La DO du sous-groupe CG était inférieure à celle du sous-groupe CN ( = 0,019). La DO du sous-groupe FG était inférieure à celle du sous-groupe FN ( = 0,02). Les DO des sous-groupes CG, CN, FG, et FN étaient inférieures à celles des témoins négatifs ( < 0,003, < 0,001, < 0,001, et < 0,00). L’utilisation d’une gaze stérile mouillée a réduit de manière significative la contamination de matériel de suture. Un patron de suture inversé avec épaisseur partielle n’a pas entrainé moins de contamination qu’un patron de suture par apposition avec pleine épaisseur.(Traduit par Docteur Serge Messier).
Publication Date: 2017-02-06 PubMed ID: 28154467PubMed Central: PMC5220602
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- Journal Article
Summary
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The study examines the impact of using a wet sterile gauze for cleaning surgical materials on contamination levels during procedures for closing pelvic flexure enterotomies in horses. The research concluded that the cleaning process significantly reduced the contaminant levels, regardless of the suture pattern used.
Study Methodology and Grouping
- The experiment involved large colon specimens, divided into two groups based on the suture patterns used, group F (Full thickness appositional suture pattern), and group C (Partial thickness inverting or Cushing suture pattern).
- Each of these groups was further divided into two subgroups, N and G. Subgroup G had a wet sterile gauze passed over the suture material, surgical instruments, and gloves, while subgroup N had no such treatment.
Data Measurement and Results
- Bacterial concentration was quantified using optical density (OD) measurements at 24 hours.
- The OD from subgroup G – both CG and FG – was less than their corresponding N subgroups. This indicates less bacterial contamination in the samples where a wet sterile gauze was used.
- Even the OD from the N subgroups was lower than the negative controls, proving that cleaning with a wet sterile gauze significantly reduces contamination.
- The type of suture pattern chosen (F or C) did not make a significant difference in contamination levels, proving that the cleaning process was the main factor affecting contamination.
Implications of the Study
- The results of the study suggest that the use of a wet sterile gauze effectively reduces the contamination of suture materials during the surgical procedures, and thus potentially minimizes postoperative complications.
- Further, the study indicated that the type of suture pattern chosen had no significant impact on the level of contamination, thus allowing surgeons freedom to choose based on other surgical considerations.
Cite This Article
APA
Giusto G, Tramuta C, Caramello V, Comino F, Nebbia P, Robino P, Singer E, Grego E, Gandini M.
(2017).
Cleaning with a wet sterile gauze significantly reduces contamination of sutures, instruments, and surgical gloves in an ex-vivo pelvic flexure enterotomy model in horses.
Can J Vet Res, 81(1), 69-72.
Publication
Researcher Affiliations
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
- Department of Veterinary Sciences, University of Turin, Largo P. Braccini 2-5, 10095 Grugliasco (TO), Italy (Giusto, Tramuta, Caramello, Comino, Nebbia, Robino, Grego, Gandini); Institute for Aging and Chronic Disease, School of Veterinary Science, University of Liverpool, Leahurst, Neston, Cheshire, United Kingdom (Singer). Marco Gandini and Elena Grego share last name authorship.
MeSH Terms
- Animals
- Bacteria
- Colon / surgery
- Enterostomy / veterinary
- Gloves, Surgical / microbiology
- Gloves, Surgical / veterinary
- Horses / surgery
- Surgical Instruments / microbiology
- Surgical Instruments / veterinary
- Sutures / microbiology
Conflict of Interest Statement
statement The authors declare that they have no conflict of interest in the publishing of this article.
References
This article includes 20 references
- Owens CD, Stoessel K. Surgical site infections: epidemiology, microbiology and prevention.. J Hosp Infect 2008 Nov;70 Suppl 2:3-10.
- Dancer SJ, Stewart M, Coulombe C, Gregori A, Virdi M. Surgical site infections linked to contaminated surgical instruments.. J Hosp Infect 2012 Aug;81(4):231-8.
- Dohmen PM. Antibiotic resistance in common pathogens reinforces the need to minimise surgical site infections.. J Hosp Infect 2008 Nov;70 Suppl 2:15-20.
- Hoshino S, Yoshida Y, Tanimura S, Yamauchi Y, Noritomi T, Yamashita Y. A study of the efficacy of antibacterial sutures for surgical site infection: a retrospective controlled trial.. Int Surg 2013 Apr-Jun;98(2):129-32.
- Edmiston CE Jr, Krepel CJ, Marks RM, Rossi PJ, Sanger J, Goldblatt M, Graham MB, Rothenburger S, Collier J, Seabrook GR. Microbiology of explanted suture segments from infected and noninfected surgical patients.. J Clin Microbiol 2013 Feb;51(2):417-21.
- Katz S, Izhar M, Mirelman D. Bacterial adherence to surgical sutures. A possible factor in suture induced infection.. Ann Surg 1981 Jul;194(1):35-41.
- Otten JE, Wiedmann-Al-Ahmad M, Jahnke H, Pelz K. Bacterial colonization on different suture materials--a potential risk for intraoral dentoalveolar surgery.. J Biomed Mater Res B Appl Biomater 2005 Jul;74(1):627-35.
- Fowler JR, Perkins TA, Buttaro BA, Truant AL. Bacteria adhere less to barbed monofilament than braided sutures in a contaminated wound model.. Clin Orthop Relat Res 2013 Feb;471(2):665-71.
- Kathju S, Nistico L, Tower I, Lasko LA, Stoodley P. Bacterial biofilms on implanted suture material are a cause of surgical site infection.. Surg Infect (Larchmt) 2014 Oct;15(5):592-600.
- Kathju S, Nistico L, Hall-Stoodley L, Post JC, Ehrlich GD, Stoodley P. Chronic surgical site infection due to suture-associated polymicrobial biofilm.. Surg Infect (Larchmt) 2009 Oct;10(5):457-61.
- Mair TS. Contributions to an evidence-based medicine approach to colic surgery.. Equine Vet J 2002 Jul;34(5):428-9.
- Mair TS, Smith LJ, Sherlock CE. Evidence-based gastrointestinal surgery in horses.. Vet Clin North Am Equine Pract 2007 Aug;23(2):267-92.
- Merritt K, Hitchins VM, Neale AR. Tissue colonization from implantable biomaterials with low numbers of bacteria.. J Biomed Mater Res 1999 Mar 5;44(3):261-5.
- Comino F, Giusto G, Caramello V, Gandini M. Techniques for pelvic flexure enterotomy closure in horses. A survey of ECVS and ACVS diplomats.. Vet Surg 2015;44:E14.
- Gandini M, Iotti BN, Giusto G. Biomechanical comparison of four technique for pelvic flexure enterotomy closure in horses.. Vet Surg 2013 Oct;42(7):892-7.
- Saito Y, Kobayashi H, Uetera Y, Yasuhara H, Kajiura T, Okubo T. Microbial contamination of surgical instruments used for laparotomy.. Am J Infect Control 2014 Jan;42(1):43-7.
- Masini BD, Stinner DJ, Waterman SM, Wenke JC. Bacterial adherence to suture materials.. J Surg Educ 2011 Mar-Apr;68(2):101-4.
- Keen JN, Austin M, Huang LS, Messing S, Wyatt JD. Efficacy of soaking in 70% isopropyl alcohol on aerobic bacterial decontamination of surgical instruments and gloves for serial mouse laparotomies.. J Am Assoc Lab Anim Sci 2010 Nov;49(6):832-7.
- Chu CC, Williams DF. Effects of physical configuration and chemical structure of suture materials on bacterial adhesion. A possible link to wound infection.. Am J Surg 1984 Feb;147(2):197-204.
- Henry-Stanley MJ, Hess DJ, Barnes AM, Dunny GM, Wells CL. Bacterial contamination of surgical suture resembles a biofilm.. Surg Infect (Larchmt) 2010 Oct;11(5):433-9.
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