Clinical, bacteriologic, serologic, and pathologic features of infections with atypical Taylorella equigenitalis in mares.
Abstract: To characterize clinical, serologic, bacteriologic, cytologic, and pathologic endometrial responses of mares to 2 donkey-origin atypical bacterial isolates resembling Taylorella equigenitalis. Methods: Prospective in vivo study. Methods: 10 healthy mares. Methods: Mares in estrus (2/group) were inoculated by intrauterine infusion with 2 isolates of classic T equigenitalis or 2 isolates of atypical Taylorella sp or were sham-inoculated. Bacteriologic, serologic, clinical, uterine, cytologic, and pathologic endometrial responses were assessed 4, 11, 21, 35, and 63 days after inoculation and on day 111 in mares with positive culture results on day 63. Results: One atypical isolate failed to cause infection. The second atypical isolate and both classic T equigenitalis isolates induced similar transient metritis and cervicitis. Both classic isolates and 1 atypical isolate induced anti-T equigenitalis complement-fixing antibodies detectable at day 11. Classic isolates and an atypical isolate provoked intense neutrophilic endometritis followed by a resolving, subacute, neutrophilic-mononuclear endometrial response. The atypical isolate and classic isolates were recovered from the uterus, clitoral fossa, or clitoral sinus of one or both exposed mares for as long as 111 days. Conclusions: Atypical Taylorella sp infections should be considered as a differential diagnosis of equine infertility in US-origin mares, even those not exposed to stallions from countries where contagious equine metritis occurs. The origins and prevalence of atypical Taylorella sp infection in US horses and donkeys are undetermined.
Publication Date: 2000-06-23 PubMed ID: 10863594DOI: 10.2460/javma.2000.216.1945Google Scholar: Lookup
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- Journal Article
Summary
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The research article presents a study examining the clinical, bacteriological, serological, and pathological responses of mares to atypical Taylorella equigenitalis, a bacteria often associated with equine infertility. The researchers found that these atypical strains could cause persistent infection and provoke immune responses similar to typical strains, suggesting they should be considered in diagnoses of equine infertility.
Research Methods
- The study was a prospective in vivo experiment involving 10 healthy mares.
- The mares, all in estrus, were divided into groups and inoculated via intrauterine infusion with either two isolates of the typical bacteria Taylorella equigenitalis, two isolates of atypical Taylorella, or were sham-inoculated as a control group.
- The researchers evaluated the mares’ bacteriological, serological, clinical, uterine, cytological, and pathological endometrial responses at intervals up to 111 days post-inoculation.
Results
- One atypical isolate did not cause any infection, while the second atypical isolate and the classic T equigenitalis isolates resulted in similar instances of transient metritis and cervicitis.
- Both the classic isolates and the one viable atypical isolate prompted an immune response indicated by the presence of anti-T equigenitalis complement-fixing antibodies detectable at day 11.
- The inoculated mares experienced intense neutrophilic endometritis followed by a resolving, subacute, neutrophilic-mononuclear endometrial response.
- The atypical and classic bacteria strains were found persisting in various parts of the horses’ reproductive tracts up to 111 days after inoculation.
Conclusion
- The researchers concluded that atypical Taylorella equigenitalis infections should be considered in the differential diagnoses of equine infertility, including in mares not exposed to stallions from countries where contagious equine metritis is common.
- The exact origins and prevalence of atypical Taylorella infections in US horses and donkeys are still uncertain and warrant further investigation.
Cite This Article
APA
Katz JB, Evans LE, Hutto DL, Schroeder-Tucker LC, Carew AM, Donahue JM, Hirsh DC.
(2000).
Clinical, bacteriologic, serologic, and pathologic features of infections with atypical Taylorella equigenitalis in mares.
J Am Vet Med Assoc, 216(12), 1945-1948.
https://doi.org/10.2460/javma.2000.216.1945 Publication
Researcher Affiliations
- National Veterinary Services Laboratories, USDA, Ames, IA 50010, USA.
MeSH Terms
- Animals
- Antibodies, Bacterial / blood
- Endometritis / microbiology
- Endometritis / pathology
- Endometritis / veterinary
- Endometrium / pathology
- Equidae
- Female
- Gram-Negative Bacterial Infections / microbiology
- Gram-Negative Bacterial Infections / pathology
- Gram-Negative Bacterial Infections / veterinary
- Horse Diseases / immunology
- Horse Diseases / microbiology
- Horse Diseases / pathology
- Horses
- Prospective Studies
- Taylorella equigenitalis / immunology
- Taylorella equigenitalis / isolation & purification
- Taylorella equigenitalis / pathogenicity
Citations
This article has been cited 10 times.- Tyrnenopoulou P, Fthenakis GC. Clinical Aspects of Bacterial Distribution and Antibiotic Resistance in the Reproductive System of Equids. Antibiotics (Basel) 2023 Mar 28;12(4).
- Nadin-Davis S, Knowles MK, Burke T, Böse R, Devenish J. Comparison of culture versus quantitative real-time polymerase chain reaction for the detection of Taylorella equigenitalis in field samples from naturally infected horses in Canada and Germany. Can J Vet Res 2015 Jul;79(3):161-9.
- Kinoshita Y, Niwa H, Katayama Y, Hariu K. Development of loop-mediated isothermal amplification methods for detecting Taylorella equigenitalis and Taylorella asinigenitalis. J Equine Sci 2015;26(1):25-9.
- Allombert J, Vianney A, Laugier C, Petry S, Hébert L. Survival of taylorellae in the environmental amoeba Acanthamoeba castellanii. BMC Microbiol 2014 Mar 19;14:69.
- Hara Y, Hayashi K, Nakajima T, Kagawa S, Tazumi A, Moore JE, Matsuda M. Molecular identification and characterization of clustered regularly interspaced short palindromic repeat (CRISPR) gene cluster in Taylorella equigenitalis. Folia Microbiol (Praha) 2013 Sep;58(5):375-84.
- Hébert L, Moumen B, Pons N, Duquesne F, Breuil MF, Goux D, Batto JM, Laugier C, Renault P, Petry S. Genomic characterization of the Taylorella genus. PLoS One 2012;7(1):e29953.
- Brooks BW, Lutze-Wallace CL, Maclean LL, Vinogradov E, Perry MB. Identification and differentiation of Taylorella equigenitalis and Taylorella asinigenitalis by lipopolysaccharide O-antigen serology using monoclonal antibodies. Can J Vet Res 2010 Jan;74(1):18-24.
- Tazumi A, Sekizuka T, Moore JE, Millar BC, Taneike I, Matsuda M. Molecular characterization of intervening sequences in 23S rRNA genes and 23S rRNA fragmentation in Taylorella equigenitalis. Folia Microbiol (Praha) 2008;53(6):486-92.
- Tazumi A, Ono S, Sekizuka T, Moore JE, Millar BC, Matsuda M. Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of Taylorella asinigenitalis. BMC Res Notes 2009 Mar 3;2:33.
- Matsuda M, Tazumi A, Kagawa S, Sekizuka T, Murayama O, Moore JE, Millar BC. Homogeneity of the 16S rDNA sequence among geographically disparate isolates of Taylorella equigenitalis. BMC Vet Res 2006 Jan 6;2:1.
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