Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin.
Abstract: Accurate quantitative analysis of equine insulin in blood samples is critical for assessing hyperinsulinemia in horses. Although there are various laboratory methods for evaluating equine serum insulin, different immunoassays show significant discrepancies between the determined insulin concentrations and are often not comparable. The aim of this study was to evaluate the Immulite® 1000 chemiluminescent immunoassay (CLIA) to establish independent laboratory and assay-specific cut values to provide an accurate diagnosis of hyperinsulinemia in horses. Thus, the analytical and clinical performance of Immulite® 1000 CLIA in terms of precision (intra- and inter-assay coefficient of variance, CV) and recovery upon dilution were evaluated and compared with radioimmunoassay (RIA), which has been previously validated for use in horses. Unassigned: Archived serum samples ( = 106) from six Quarter horse mares enrolled in the glucose phase of a Frequently Sampled Insulin and Glucose Test (FSIGT) study were used to measure blood insulin. Unassigned: The Immulite® 1000 CLIA had good precision with acceptable intra- and inter-assay CVs, adequate recovery on dilution, and a strong correlation with the RIA ( = 0.974, < 0.0001), with constant bias resulting in consistently lower values. Unassigned: On this basis, the Immulite® 1000 Insulin Assay is valid for measuring equine serum insulin for diagnostic and monitoring purposes when cut values are appropriately adjusted.
Copyright © 2023 Go, Hazard, Balasuriya, Chapman, Fitton, Kenéz and Andrews.
Publication Date: 2023-03-27 PubMed ID: 37051514PubMed Central: PMC10083388DOI: 10.3389/fvets.2023.1018230Google Scholar: Lookup
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- Journal Article
Summary
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The research article presents a study evaluating the performance of Immulite® 1000, a chemiluminescent immunoassay, in measuring insulin levels in horse serum, with a focus on its precision and recovery upon dilution.
Objectives of the Study
- The main purpose of this study was to assess the effectiveness of the Immulite® 1000 chemiluminescent immunoassay (CLIA) for accurately determining equine serum insulin levels, which is crucial for diagnosing hyperinsulinemia in horses.
- The researchers aimed to establish laboratory-specific and assay-specific cut-off values with this method as different existing methods often show inconsistencies in results.
Methods
- The Immulite® 1000 CLIA’s analytical and clinical performance was evaluated based on its precision (using intra- and inter-assay coefficients of variance, CV) and its ability to recover upon dilution.
- These values were compared to the radioimmunoassay (RIA), a method previously validated for use in horses.
- The study utilized archived serum samples from six Quarter horse mares who had participated in the glucose phase of a Frequently Sampled Insulin and Glucose Test (FSIGT) study.
Findings
- The study found that the Immulite® 1000 CLIA showed good precision with acceptable intra- and inter-assay CVs, implying its ability to provide consistent results over multiple measurements carried out under identical conditions.
- The CLIA also showed satisfactory recovery upon dilution, important for maintaining accuracy and reliability in measurements with different concentrations.
- The assay’s performance in measuring equine serum insulin strongly correlated with results from the RIA method ( = 0.974, < 0.0001).
- However, the researchers identified a constant bias in the CLIA, in that it consistently returned lower values.
Conclusions
- The study concluded that the Immulite® 1000 Insulin Assay is a valid technique for quantifying equine serum insulin for diagnostic and monitoring purposes.
- However, to ensure accurate diagnosis of hyperinsulinemia in horses, cut-off values must be adjusted according to this assay’s consistent bias toward lower values.
Cite This Article
APA
Go YY, Hazard NW, Balasuriya UBR, Chapman AM, Fitton NS, Kenéz Á, Andrews FM.
(2023).
Clinical evaluation of the Immulite® 1000 chemiluminescent immunoassay for measurement of equine serum insulin.
Front Vet Sci, 10, 1018230.
https://doi.org/10.3389/fvets.2023.1018230 Publication
Researcher Affiliations
- Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
- Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States.
- Louisiana Animal Disease Diagnostic Laboratory and Department of Pathobiological Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States.
- Equine Health Studies Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States.
- Siemens Healthcare Diagnostics Inc., Tarrytown, NY, United States.
- Department of Infectious Diseases and Public Health, Jockey Club College of Veterinary Medicine and Life Sciences, City University of Hong Kong, Kowloon, Hong Kong SAR, China.
- Equine Health Studies Program, Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA, United States.
Conflict of Interest Statement
NF was employed by Siemens Healthcare Diagnostics Inc. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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