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Home / Equine Research Bank / Infect Immun / Clinical, histopathological, and immunological responses of ...
Infection and immunity1988; 56(11); 2960-2966; doi: 10.1128/iai.56.11.2960-2966.1988

Clinical, histopathological, and immunological responses of ponies to Ehrlichia sennetsu and subsequent Ehrlichia risticii challenge.

Abstract: Ehrlichia risticii has a close antigenic relationship to E. sennetsu. Sera of ponies experimentally infected with E. risticii, the etiologic agent of Potomac horse fever, consistently reacted with E. sennetsu, a human pathogen, in indirect fluorescent-antibody (IFA) testing, while human E. sennetsu convalescent serum reacted with E. risticii by IFA testing and immunoferritin labeling of cells infected in vitro. Two ponies injected intravenously with live E. sennetsu did no develop clinical illness. Subsequent injection with live E. sennetsu did not develop clinical illness. Subsequent injection with live E. risticii also did not induce any disease, in contrast to two control ponies given E. risticii without prior exposure to E. sennetsu. Both controls developed fever, anorexia, depression, dehydration, and diarrhea, which are typical clinical signs of Potomac horse fever, and had characteristic lesions of enteritis and lymph node histiocytosis at postmortem examination. E. sennetsu-exposed ponies had normal gastrointestinal morphologies and lymph node hyperplasia. Ponies primed with E. sennetsu before E. risticii challenge developed high titers of immunoglobulin G antibody which reacted against both E. sennetsu and E. risticii antigens by IFA testing. The most prominent antigenic polypeptide in Western (immuno-) blot analysis of sera collected from ponies primed with E. sennetsu before subsequent challenge with E. risticii was present in lysates of both Ehrlichia species and had an apparent molecular mass of 44 kilodaltons. This band was not prominent in Western blots performed with sera of ponies injected with E. risticii alone. Thus, injection of E. sennetsu protects ponies from clinical and pathological manifestations of the disease induced by injection with E. risticii. Immunologic cross-reactivity of the two organisms with IFA testing and strong immunologic recognition by ponies of the 44-kilodalton antigen common to the two organisms may be related to the development of protective immunity against E. risticii.
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Publication Date: 1988-11-01 PubMed ID: 3169993PubMed Central: PMC259677DOI: 10.1128/iai.56.11.2960-2966.1988Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates the immunological responses of ponies to Ehrlichia sennetsu and subsequent Ehrlichia risticii infection. It reveals that introducing E. sennetsu to the ponies led to protection against clinical and pathological effects of E. risticii infection.

Objective and Methodology

  • The study aimed to understand the immunological relationship between the pathogens Ehrlichia sennetsu and Ehrlichia risticii, focusing on the response in ponies.
  • Two ponies were injected with the E. sennetsu pathogen, monitored for clinical illness and then subsequently injected with E. risticii.
  • These two experimental groups were compared against two control ponies that were injected with only E. risticii.

Findings and Results

  • The study reported that ponies injected with E. sennetsu did not develop any illness. Injecting these ponies with E. risticii after did not result in any disease, unlike the control ponies which exhibited signs of Potomac horse fever such as fever, anorexia, depression, dehydration, and diarrhea.
  • This contrast indicated that prior exposure to E. sennetsu provided some level of protection against the effects of E. risticii.
  • The control ponies showed lesions and lymph node hyperplasia. In contrast, the ponies infected with E. sennetsu had normal gastrointestinal morphologies and lymph node hyperplasia.

Immunological Cross-Reactivity and Protection

  • The study also discovered that E. sennetsu priming helped develop a high level of immunoglobulin G antibody generation against both E. sennetsu and E. risticii.
  • The most prominent antigenic polypeptide, present in the sera of ponies primed with E. sennetsu and then exposed to E. risticii, was found in lysates of both Ehrlichia species and had a molecular mass of 44 kilodaltons.
  • This antigenic band was not present when E. risticii alone was injected, suggesting its connection to the development of protective immunity against E. risticii.

Significance of the Study

  • The study provides valuable insights about the disease mechanisms associated with the pathogens E. sennetsu and E. risticii, particularly regarding their effect on ponies.
  • The results suggest that E. sennetsu infection might offer some level of immunity or protection against E. risticii, rendering beneficial potential for future preventive or therapeutic strategies concerning E. risticii induced diseases.

Cite This Article

APA
Rikihisa Y, Pretzman CI, Johnson GC, Reed SM, Yamamoto S, Andrews F. (1988). Clinical, histopathological, and immunological responses of ponies to Ehrlichia sennetsu and subsequent Ehrlichia risticii challenge. Infect Immun, 56(11), 2960-2966. https://doi.org/10.1128/iai.56.11.2960-2966.1988

Publication

Infection and immunity
ISSN: 0019-9567
NlmUniqueID: 0246127
Country: United States
Language: English
Volume: 56
Issue: 11
Pages: 2960-2966

Researcher Affiliations

Rikihisa, Y
  • Department of Veterinary Pathobiology, College of Veterinary Medicine, Ohio State University, Columbus 43210-1092.
Pretzman, C I
    Johnson, G C
      Reed, S M
        Yamamoto, S
          Andrews, F

            MeSH Terms

            • Animals
            • Antibodies, Bacterial / immunology
            • Antigens, Bacterial / immunology
            • Cross Reactions
            • Ehrlichia / immunology
            • Horse Diseases / immunology
            • Horse Diseases / pathology
            • Horse Diseases / prevention & control
            • Horses / immunology
            • Immunization
            • Molecular Weight
            • Rickettsiaceae / immunology
            • Rickettsiaceae Infections / immunology
            • Rickettsiaceae Infections / pathology
            • Rickettsiaceae Infections / prevention & control
            • Rickettsiaceae Infections / veterinary
            • Species Specificity

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            Citations

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