Collagenase in equine cell culture preparation.
Abstract: Equine kidney cells disaggregated by treatment with 0.01% collagenase were used in the preparation of primary monolayer cell cultures. The primary cells could be stored for long periods in liquid nitrogen and subsequently subcultivated. These techniques provided a long-term supply of equine kidney cells, free of apparent contamination, from the kidneys of a single fetus.
Publication Date: 1979-06-01 PubMed ID: 227924PubMed Central: PMC275389DOI: 10.1128/jcm.9.6.731-733.1979Google Scholar: Lookup
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- Journal Article
Summary
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The research article is about a process for preparing equine kidney cell cultures using collagenase, with the cells being disaggregated by a 0.01% collagenase treatment, stored in liquid nitrogen for an extended time, and then subcultivated, resulting in a contamination-free, long-term supply of these cells from a single fetal kidney.
Introduction
The study aims at developing a reliable method of producing uncontaminated, long-term supplies of equine kidney cells. The researchers undertook an approach involving the use of 0.01% collagenase to disaggregate equine kidney cells and the storage of primary cells in liquid nitrogen.
Methodology
- The researchers commenced by treating equine kidney cells with 0.01% collagenase, a substance used to break down collagen, a protein found commonly in the body tissues of vertebrates.
- Upon successful disaggregation (the separation of the individual cells), the researchers proceeded to prepare primary monolayer cell cultures. This generally involves the cultivation of cells in a flask until they form a single-layer of cells covering the entire bottom surface.
Cell Storage and Cultivation
- The team then stored these primary cells in liquid nitrogen for an extended period. Such storage is typically known as cryopreservation and is used to maintain cellular integrity over long periods.
- After storage, the researchers carried out subcultivation, a technique that involves the transfer of cells from a previous culture into fresh growth medium, in this case, equine kidney cells, to propagate a continuous line of cells.
Key Outcomes
- Collagenase usage in equine kidney cell preparation proves efficient as it enables the disaggregation of cells without damaging them thereby preserving cellular function.
- The process facilitated long-term cell storage via cryopreservation and effective subcultivation, allowing researchers to establish a steady supply of cells for their work, sourced from the kidneys of a single fetus.
- The resulting cell cultures were not contaminated, which is a significant outcome as it ensures the reliability and purity of cultures used in further research.
Cite This Article
APA
Lang G.
(1979).
Collagenase in equine cell culture preparation.
J Clin Microbiol, 9(6), 731-733.
https://doi.org/10.1128/jcm.9.6.731-733.1979 Publication
Researcher Affiliations
MeSH Terms
- Animals
- Cell Separation
- Cells, Cultured
- Fetus
- Horses
- Kidney
- Microbial Collagenase / pharmacology
References
This article includes 16 references
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