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Home / Equine Research Bank / Reprod Domest Anim / Collagens and DNA methyltransferases in mare endometrosis.
Reproduction in domestic animals = Zuchthygiene2019; 54 Suppl 3; 46-52; doi: 10.1111/rda.13515

Collagens and DNA methyltransferases in mare endometrosis.

Abstract: Inflammation and fibroproliferative diseases may be modulated by epigenetic changes. Therefore, we suggest that epigenetic mechanisms could be involved in equine endometrosis pathogenesis. DNA methylation is one of the methods to evaluate epigenetics, through the transcription of methyltransferases (DNMT1, DNMT3A, DNMT3B). The correlation between DNMTs and collagen (COL) transcripts was assessed for the different Kenney and Doig's (Current Therapy in Theriogenology. Philadelphia: WB Saunders; 1986) endometrium categories. Endometrial biopsies were randomly collected from cyclic mares. Histological classification (category I, n = 13; II A, n = 17; II B, n = 12; and III, n = 7) and evaluation of COL1A2, COL3A1 and DNMTs transcripts by qPCR, were performed. Data were analysed by one-way analysis of variance (ANOVA), Kruskal-Wallis test and Pearson correlation. As mares aged, there was an increase in endometrium fibrosis (p < .01), and in DNMT1 mRNA (p < .001). Considering DNMT3B transcripts for each category, there was an increase with fibrosis (p < .05). No changes were observed for DNMT1 and DNMT3A transcripts. However, DNMT3A mRNA levels were the highest in all categories (p < .01). In category I endometrium, a positive correlation was observed for transcripts of all DNMTs in both COLs (p < .01). In category IIA, this correlation was also maintained for all DNMTs transcripts in COL1A2 (p < .05), but only for DNMT3B in COL3A1 (p < .05). In category IIB, there was a positive correlation between DNMT3B and COL3A1 (p < .05). In category III, a positive correlation was only observed between DNMT3B and COL3A1 (p < .05). Our results suggest that there is a disturbance in COLs and DNMTs correlation during fibrosis.
© 2019 Blackwell Verlag GmbH.
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Publication Date: 2019-09-13 PubMed ID: 31512314DOI: 10.1111/rda.13515Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research paper examines the involvement of epigenetic mechanisms, particularly DNA methylation, in the pathogenesis of endometrosis in mares. It investigates the correlation between certain collagen transcripts and DNA methyltransferases, uncovering a disruption in their relationship during fibrosis.

Study Design and Procedure

  • The study focused on assessing how the transcription of DNA methyltransferases (DNMT1, DNMT3A, DNMT3B) affects the development of endometrosis in mares. Endometrosis is classified into different categories according to Kenney and Doig’s classification system.
  • Endometrial biopsies were obtained from mares during their reproductive cycle and then categorized according to the severity of fibrosis and inflammation – category I (n = 13), II A (n=17), II B (n=12), and III (n=7).
  • The researchers examined the transcript levels of the collagens COL1A2 and COL3A1, along with DNMTs, using quantitative polymerase chain reaction (qPCR).
  • The gathered data were analyzed using several statistical methods – one-way analysis of variance (ANOVA), the Kruskal-Wallis test, and a Pearson correlation analysis.

Main Findings

  • The study found that as mares age, there’s an increase in endometrium fibrosis severity and in DNMT1 mRNA.
  • DNMT3B transcript levels for each endometrosis category increased with fibrosis severity.
  • While no changes were observed for DNMT1 and DNMT3A transcripts, DNMT3A mRNA levels were significantly higher across all categories.
  • In the healthiest endometria (category I), a positive correlation was observed for the transcripts of all DNMTs in both collagens. This correlation was maintained for all DNMT transcripts in COL1A2 (p < .05), but only for DNMT3B in COL3A1 in category IIA.
  • In categories IIB and III, there was a positive correlation between DNMT3B and COL3A1.

Conclusion

  • The results suggest that there is a disturbance in the correlation between collagens and DNA methyltransferases during the development of fibrosis, implying that DNA methylation may play a significant role in the pathogenesis of endometrosis in mares.

Cite This Article

APA
Alpoim-Moreira J, Fernandes C, Rebordão MR, Amaral A, Pinto-Bravo P, Bliebernicht M, Skarzynski DJ, Ferreira-Dias G. (2019). Collagens and DNA methyltransferases in mare endometrosis. Reprod Domest Anim, 54 Suppl 3, 46-52. https://doi.org/10.1111/rda.13515

Publication

Reproduction in domestic animals = Zuchthygiene
ISSN: 1439-0531
NlmUniqueID: 9015668
Country: Germany
Language: English
Volume: 54 Suppl 3
Pages: 46-52

Researcher Affiliations

Alpoim-Moreira, Joana
  • Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Fernandes, Carina
  • Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Rebordão, Maria Rosa
  • Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
  • Coimbra College of Agriculture, Polytechnic Institute of Coimbra, Coimbra, Portugal.
Amaral, Ana
  • Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.
Pinto-Bravo, Pedro
  • Coimbra College of Agriculture, Polytechnic Institute of Coimbra, Coimbra, Portugal.
Bliebernicht, Miguel
  • Embriovet, Muge, Portugal.
Skarzynski, Dariusz J
  • Polish Academy of Science, Olsztyn, Poland.
Ferreira-Dias, Graça
  • Centre for Interdisciplinary Research in Animal Health, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal.

MeSH Terms

  • Aging / physiology
  • Animals
  • Collagen / genetics
  • Collagen / metabolism
  • DNA (Cytosine-5-)-Methyltransferases / genetics
  • DNA Methylation
  • Endometritis / genetics
  • Endometritis / metabolism
  • Endometritis / veterinary
  • Endometrium / pathology
  • Female
  • Fibrosis / physiopathology
  • Horse Diseases / genetics
  • Horse Diseases / metabolism
  • Horses
  • RNA, Messenger

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Citations

This article has been cited 6 times.
  1. Dementieva N, Nikitkina E, Shcherbakov Y, Nikolaeva O, Mitrofanova O, Ryabova A, Atroshchenko M, Makhmutova O, Zaitsev A. The Genetic Diversity of Stallions of Different Breeds in Russia.. Genes (Basel) 2023 Jul 24;14(7).
    doi: 10.3390/genes14071511pubmed: 37510415google scholar: lookup
  2. Alpoim-Moreira J, Szóstek-Mioduchowska A, Słyszewska M, Rebordão MR, Skarzynski DJ, Ferreira-Dias G. 5-Aza-2'-Deoxycytidine (5-Aza-dC, Decitabine) Inhibits Collagen Type I and III Expression in TGF-β1-Treated Equine Endometrial Fibroblasts.. Animals (Basel) 2023 Mar 30;13(7).
    doi: 10.3390/ani13071212pubmed: 37048467google scholar: lookup
  3. Zdrojkowski Ł, Jasiński T, Ferreira-Dias G, Pawliński B, Domino M. The Role of NF-κB in Endometrial Diseases in Humans and Animals: A Review.. Int J Mol Sci 2023 Feb 2;24(3).
    doi: 10.3390/ijms24032901pubmed: 36769226google scholar: lookup
  4. Alpoim-Moreira J, Fernandes C, Pimenta J, Bliebernicht M, Rebordão MR, Castelo-Branco P, Szóstek-Mioduchowska A, Skarzynski DJ, Ferreira-Dias G. Metallopeptidades 2 and 9 genes epigenetically modulate equine endometrial fibrosis.. Front Vet Sci 2022;9:970003.
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  5. Katila T, Ferreira-Dias G. Evolution of the Concepts of Endometrosis, Post Breeding Endometritis, and Susceptibility of Mares.. Animals (Basel) 2022 Mar 19;12(6).
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  6. Canisso IF, Segabinazzi LGTM, Fedorka CE. Persistent Breeding-Induced Endometritis in Mares - a Multifaceted Challenge: From Clinical Aspects to Immunopathogenesis and Pathobiology.. Int J Mol Sci 2020 Feb 20;21(4).
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