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The Veterinary clinics of North America. Equine practice2011; 27(2); 263-274; doi: 10.1016/j.cveq.2011.05.003

Collection and propagation methods for mesenchymal stromal cells.

Abstract: Mesenchymal stromal cells (MSC) are derived from adult mesenchymal tissues and have the ability to undergo differentiation into bone, cartilage, and fat, and have therefore attracted great interest in regenerative medicine. Many isolation and culture methods have been described, making comparison between laboratories and quality-control protocols difficult. A uniform protocol to characterize equine MSC has recently been proposed, aiming to introduce consistency across the equine stem cell research field. This article reviews the published techniques for collection and propagation of equine MSC, focusing on bone marrow-derived and adipose-derived cells.
Copyright © 2011 Elsevier Inc. All rights reserved.
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Publication Date: 2011-08-30 PubMed ID: 21872758DOI: 10.1016/j.cveq.2011.05.003Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article focuses on the collection and propagation methods for mesenchymal stromal cells (MSC), derived from adult tissues and used in regenerative medicine, and the development of a consistent protocol for characterizing MSC, particularly those derived from equine bone marrow and adipose cells.

Understanding MSC and their Role in Regenerative Medicine

  • The article emphasizes the potential utility and significance of mesenchymal stromal cells (MSC) in regenerative medicine. MSCs are adult stem cells that originate from mesenchymal tissues, which are a variety of connective tissues found in the body.
  • These cells are capable of differentiating into several types of cells such as bone, cartilage, and fat cells. This characteristic allows the potential use of MSCs in therapies to regenerate damaged tissues.

Issue of Inconsistent Isolation and Culture Methodologies

  • The research points out a critical issue in the current landscape of MSC research: the lack of consistency in isolation and culture methods. Different laboratories follow different methods to isolate and propagate these cells.
  • Due to these discrepancies, comparing results between laboratories becomes challenging. Moreover, it also hampers the establishment of quality-control protocols that rely on uniform practices.

Introducing a Uniform Protocol

  • The study discusses the recent proposal of a standard protocol to characterize equine MSC. This could be pivotal in ensuring consistency in research procedures related to MSC.
  • By applying the same collection and propagation techniques, researchers can ensure the replicability of their studies across different laboratories, which is essential for validating results.

Focus on Equine Bone Marrow-derived and Adipose-derived MSCs

  • Specifically, the authors focus on equine MSCs that are derived from bone marrow and adipose (fat) tissues. These types of MSCs are of particular interest because they can be collected non-invasively and propagated in large quantities, making them a feasible option for use in clinical therapies.
  • Compiling and reviewing various techniques to collect and propagate these specific cells can provide a substantial basis for developing a uniform protocol.

Cite This Article

APA
Taylor SE, Clegg PD. (2011). Collection and propagation methods for mesenchymal stromal cells. Vet Clin North Am Equine Pract, 27(2), 263-274. https://doi.org/10.1016/j.cveq.2011.05.003

Publication

The Veterinary clinics of North America. Equine practice
ISSN: 1558-4224
NlmUniqueID: 8511904
Country: United States
Language: English
Volume: 27
Issue: 2
Pages: 263-274

Researcher Affiliations

Taylor, Sarah E
  • Department of Veterinary Clinical Sciences, University of Edinburgh, Dick Vet Equine Hospital, Easter Bush Vet Centre, Roslin, Midlothian, EH25 9RG, UK. Sarah.e.taylor@ed.ac.uk
Clegg, Peter D

    MeSH Terms

    • Animals
    • Cell Differentiation
    • Horses / physiology
    • Mesenchymal Stem Cells / cytology
    • Mesenchymal Stem Cells / physiology
    • Specimen Handling

    Citations

    This article has been cited 10 times.
    1. Saberian M, Shahidi Delshad E. The role of Spirulina platensis on the proliferation of rat bone marrow-derived mesenchymal stem cells. Iran J Microbiol 2023 Feb;15(1):111-120.
      doi: 10.18502/ijm.v15i1.11925pubmed: 37069913google scholar: lookup
    2. Mayet A, Zablotski Y, Roth SP, Brehm W, Troillet A. Systematic review and meta-analysis of positive long-term effects after intra-articular administration of orthobiologic therapeutics in horses with naturally occurring osteoarthritis. Front Vet Sci 2023;10:1125695.
      doi: 10.3389/fvets.2023.1125695pubmed: 36908512google scholar: lookup
    3. Pilgrim CR, McCahill KA, Rops JG, Dufour JM, Russell KA, Koch TG. A Review of Fetal Bovine Serum in the Culture of Mesenchymal Stromal Cells and Potential Alternatives for Veterinary Medicine. Front Vet Sci 2022;9:859025.
      doi: 10.3389/fvets.2022.859025pubmed: 35591873google scholar: lookup
    4. Al Naem M, Bourebaba L, Kucharczyk K, Röcken M, Marycz K. Therapeutic mesenchymal stromal stem cells: Isolation, characterization and role in equine regenerative medicine and metabolic disorders. Stem Cell Rev Rep 2020 Apr;16(2):301-322.
      doi: 10.1007/s12015-019-09932-0pubmed: 31797146google scholar: lookup
    5. Bogers SH. Cell-Based Therapies for Joint Disease in Veterinary Medicine: What We Have Learned and What We Need to Know. Front Vet Sci 2018;5:70.
      doi: 10.3389/fvets.2018.00070pubmed: 29713634google scholar: lookup
    6. Geburek F, Roggel F, van Schie HTM, Beineke A, Estrada R, Weber K, Hellige M, Rohn K, Jagodzinski M, Welke B, Hurschler C, Conrad S, Skutella T, van de Lest C, van Weeren R, Stadler PM. Effect of single intralesional treatment of surgically induced equine superficial digital flexor tendon core lesions with adipose-derived mesenchymal stromal cells: a controlled experimental trial. Stem Cell Res Ther 2017 Jun 5;8(1):129.
      doi: 10.1186/s13287-017-0564-8pubmed: 28583184google scholar: lookup
    7. Esteves CL, Sheldrake TA, Dawson L, Menghini T, Rink BE, Amilon K, Khan N, Péault B, Donadeu FX. Equine Mesenchymal Stromal Cells Retain a Pericyte-Like Phenotype. Stem Cells Dev 2017 Jul 1;26(13):964-972.
      doi: 10.1089/scd.2017.0017pubmed: 28376684google scholar: lookup
    8. Rivera Orsini MA, Ozmen EB, Miles A, Newby SD, Springer N, Millis D, Dhar M. Isolation and Characterization of Canine Adipose-Derived Mesenchymal Stromal Cells: Considerations in Translation from Laboratory to Clinic. Animals (Basel) 2024 Oct 15;14(20).
      doi: 10.3390/ani14202974pubmed: 39457904google scholar: lookup
    9. Li H, Xiong S, Masieri FF, Monika S, Lethaus B, Savkovic V. Mesenchymal Stem Cells Isolated from Equine Hair Follicles Using a Method of Air-Liquid Interface. Stem Cell Rev Rep 2023 Nov;19(8):2943-2956.
      doi: 10.1007/s12015-023-10619-wpubmed: 37733199google scholar: lookup
    10. Turlo AJ, Hammond DE, Ramsbottom KA, Soul J, Gillen A, McDonald K, Peffers MJ. Mesenchymal Stromal Cell Secretome Is Affected by Tissue Source and Donor Age. Stem Cells 2023 Nov 5;41(11):1047-1059.
      doi: 10.1093/stmcls/sxad060pubmed: 37591507google scholar: lookup
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