Collection of equine cord blood and placental tissues in 40 thoroughbred mares.
Abstract: Stem cells derived from umbilical cord tissue (UCT) and umbilical cord blood (UCB) in human subjects and horses can be obtained in a minimally invasive fashion with successful propagation of mesenchymal stem cells (MSCs). Currently there are no detailed protocols documenting a procedure to harvest UCB and UCT safely for equine stem cell propagation. Objective: UCB and UCT could be collected without harm to mare or foal. Objective: To develop a standard and safe method for UCB and UCT collection, and prospectively to compare foal and mare health between groups of animals where tissue was and was not collected. Methods: This study was conducted at a Thoroughbred breeding facility in central California in 2008. UCB and UCT were collected from 40 mare and foal pairs. Clinical parameters including time for foal to stand and nurse, time for mare to pass the placenta, and foal haematology data at age 24 h were documented and compared to a control group, consisting of the succeeding 40 mare and foal pairs. Results: UCB was obtained successfully from 36 of 40 (90%) mares and UCT from 38 of 40 (95%) mares. Bacterial contamination was documented in 6 out of 36 (16.6%) UCB samples. There were no significant differences in time to stand or nurse for foals or time to pass the placenta for mares, between the experimental and control groups. There were no clinically relevant differences identified in haematological data obtained from foals with and without UCB collection. Conclusions: UCB and UCT can be harvested safely without harm to mares or foals. Conclusions: UCB and UCT samples collected in an inherently contaminated environment can be successfully disinfected and transported with minimal bacterial overgrowth for use in cell culture to isolate MSCs.
Publication Date: 2010-01-26 PubMed ID: 20095217DOI: 10.2746/042516409x429446Google Scholar: Lookup
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- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article focuses on developing a safe method to harvest umbilical cord tissue (UCT) and umbilical cord blood (UCB) from thoroughbred mares for the propagation of mesenchymal stem cells (MSCs). The study conducted in California successfully collected UCB and UCT from 40 mare and foal pairs without harm and compared their health to a control group.
Research objective and context
- This study was conducted with the aim of finding a safe and standard method to collect umbilical cord blood (UCB) and umbilical cord tissue (UCT) from mares. Such tissues are an important source of mesenchymal stem cells (MSCs), which have various medical applications.
- The researchers also wanted to check if this collection process had any harmful effects on the mares or their foals. For this, they compared the health of the group from which UCB and UCT were collected to a control group.
Methods
- UCB and UCT were collected from 40 mare and foal pairs at a Thoroughbred breeding facility in central California in 2008.
- Various clinical parameters were documented for both the groups including: time taken by the foal to stand and nurse, time taken by the mare to pass the placenta, and also foal haematology data at 24 hours of age.
Results
- The collection process was largely successful – UCB was obtained from 90% of the mares, and UCT was obtained from 95% of the mares.
- Bacterial contamination, a concern in this process, was documented in about 17% of the UCB samples.
- There was no significant difference in the time taken by the foals to stand or nurse, or by the mares to pass the placenta, between the experimental and control groups. The haematology data of the foals did not show any clinically relevant differences either.
Conclusion
- The researchers concluded that UCB and UCT can be safely collected from mares without causing any harm to them or their foals.
- Despite being collected in an environment susceptible to contamination, the samples showed minimal bacterial growth and could be successfully disinfected, thereby being suitable for use in cell culture to isolate MSCs.
Cite This Article
APA
Bartholomew S, Owens SD, Ferraro GL, Carrade DD, Lara DJ, Librach FA, Borjesson DL, Galuppo LD.
(2010).
Collection of equine cord blood and placental tissues in 40 thoroughbred mares.
Equine Vet J, 41(8), 724-728.
https://doi.org/10.2746/042516409x429446 Publication
Researcher Affiliations
- Center for Equine Health, Veterinary Medical Teaching Hospital, USA.
MeSH Terms
- Animals
- Female
- Horses / blood
- Horses / physiology
- Placenta / physiology
- Pregnancy
- Specimen Handling / veterinary
Citations
This article has been cited 8 times.- Kulus M, Sibiak R, Stefańska K, Zdun M, Wieczorkiewicz M, Piotrowska-Kempisty H, Jaśkowski JM, Bukowska D, Ratajczak K, Zabel M, Mozdziak P, Kempisty B. Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues-Origins, Characteristics, Signaling Pathways, and Clinical Trials.. Cells 2021 Nov 23;10(12).
- Magri C, Schramme M, Febre M, Cauvin E, Labadie F, Saulnier N, François I, Lechartier A, Aebischer D, Moncelet AS, Maddens S. Comparison of efficacy and safety of single versus repeated intra-articular injection of allogeneic neonatal mesenchymal stem cells for treatment of osteoarthritis of the metacarpophalangeal/metatarsophalangeal joint in horses: A clinical pilot study.. PLoS One 2019;14(8):e0221317.
- Bertoni L, Branly T, Jacquet S, Desancé M, Desquilbet L, Rivory P, Hartmann DJ, Denoix JM, Audigié F, Galéra P, Demoor M. Intra-Articular Injection of 2 Different Dosages of Autologous and Allogeneic Bone Marrow- and Umbilical Cord-Derived Mesenchymal Stem Cells Triggers a Variable Inflammatory Response of the Fetlock Joint on 12 Sound Experimental Horses.. Stem Cells Int 2019;2019:9431894.
- Cabon Q, Febre M, Gomez N, Cachon T, Pillard P, Carozzo C, Saulnier N, Robert C, Livet V, Rakic R, Plantier N, Saas P, Maddens S, Viguier E. Long-Term Safety and Efficacy of Single or Repeated Intra-Articular Injection of Allogeneic Neonatal Mesenchymal Stromal Cells for Managing Pain and Lameness in Moderate to Severe Canine Osteoarthritis Without Anti-inflammatory Pharmacological Support: Pilot Clinical Study.. Front Vet Sci 2019;6:10.
- Corradetti B, Correani A, Romaldini A, Marini MG, Bizzaro D, Perrini C, Cremonesi F, Lange-Consiglio A. Amniotic membrane-derived mesenchymal cells and their conditioned media: potential candidates for uterine regenerative therapy in the horse.. PLoS One 2014;9(10):e111324.
- Barberini DJ, Freitas NP, Magnoni MS, Maia L, Listoni AJ, Heckler MC, Sudano MJ, Golim MA, da Cruz Landim-Alvarenga F, Amorim RM. Equine mesenchymal stem cells from bone marrow, adipose tissue and umbilical cord: immunophenotypic characterization and differentiation potential.. Stem Cell Res Ther 2014 Feb 21;5(1):25.
- Carrade DD, Lame MW, Kent MS, Clark KC, Walker NJ, Borjesson DL. Comparative Analysis of the Immunomodulatory Properties of Equine Adult-Derived Mesenchymal Stem Cells().. Cell Med 2012;4(1):1-11.
- Lovati AB, Corradetti B, Lange Consiglio A, Recordati C, Bonacina E, Bizzaro D, Cremonesi F. Comparison of equine bone marrow-, umbilical cord matrix and amniotic fluid-derived progenitor cells.. Vet Res Commun 2011 Feb;35(2):103-21.
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