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Home / Equine Research Bank / Appl Environ Microbiol / Colonization of the stratified squamous epithelium of the no...
Applied and environmental microbiology2000; 66(11); 5030-5034; doi: 10.1128/AEM.66.11.5030-5034.2000

Colonization of the stratified squamous epithelium of the nonsecreting area of horse stomach by lactobacilli.

Abstract: Selective adhesion to only certain epithelia is particularly common among the bacterial members of the indigenous microflora of mammals. We have found that the stratified squamous epithelium of the nonsecreting area of horse stomach is colonized by gram-positive rods. The microscopic features of a dense layer of these bacteria on the epithelium were found to be similar to those reported in mice, rats, and swine. Adhering microorganisms were isolated and identified as Lactobacillus salivarius, L. crispatus, L. reuteri, and L. agilis by DNA-DNA hybridization and 16S rRNA gene sequencing techniques. These lactobacilli associated with the horse, except for L. reuteri, were found to adhere to horse epithelial cells in vitro but not to those of rats. A symbiotic relationship of these lactobacilli with the horse is suggested.
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Publication Date: 2000-10-31 PubMed ID: 11055960PubMed Central: PMC92416DOI: 10.1128/AEM.66.11.5030-5034.2000Google Scholar: Lookup
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  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

This research article investigates how lactobacilli bacteria colonize the nonsecreting area of a horse’s stomach, and suggests a symbiotic relationship between these bacteria and horses.

Objective and Purpose of the Study

  • The study was focused on understanding the ability of certain bacteria, specifically lactobacilli, to selectively adhere to particular epithelia in mammals. The researchers chose to focus their investigation on the stratified squamous epithelium of the nonsecreting area of horse stomach, a place they observed to be densely populated by gram-positive rod-shaped bacteria.
  • The ultimate purpose of the research was to identify these bacteria and to probe the nature of their relationship with the horse, with a hypothesis that these bacteria and the horse might have a symbiotic relationship.

Methods and Techniques Used in the Research

  • The researchers conducted microscopic investigations of the dense layer of bacteria in the horse’s stomach and found that their characteristics were similar to those reported in rodents and pigs. This suggested a proliferation of similar bacteria across multiple mammalian species.
  • The team isolated the adhering microorganisms and identified them using DNA-DNA hybridization and 16S rRNA gene sequencing techniques. These are standard biotechnological methods for identifying microbes based on their genetic material. The bacteria were identified as Lactobacillus salivarius, L. crispatus, L. reuteri, and L. agilis.
  • In vitro tests were conducted to explore the ability of these isolated lactobacilli to adhere to horse epithelial cells. The same tests were also conducted using rat epithelial cells for comparison.

Findings of the Study

  • The results confirmed that these lactobacilli could selectively adhere to equine (horse) epithelial cells versus rat epithelial cells in vitro. This suggested a species-specific interaction, pointing towards a potentially symbiotic relationship between the lactobacilli and the horse.
  • All the associated lactobacilli except L. reuteri displayed the ability to adhere to horse epithelial cells in vitro, however they did not exhibit this affinity towards rat cells.

Conclusion and Possible Implication

  • The study concluded that the selective adhesion of lactobacilli to the horse stomach’s epithelial cells suggests a mutually beneficial interaction between these microbes and the horse.
  • Though the study doesn’t explicitly confirm the nature of this symbiosis, understanding such interactions may have implications for probiotic use and targeted manipulation of the microbiome in horses for health and disease treatment.

Cite This Article

APA
Yuki N, Shimazaki T, Kushiro A, Watanabe K, Uchida K, Yuyama T, Morotomi M. (2000). Colonization of the stratified squamous epithelium of the nonsecreting area of horse stomach by lactobacilli. Appl Environ Microbiol, 66(11), 5030-5034. https://doi.org/10.1128/AEM.66.11.5030-5034.2000

Publication

Applied and environmental microbiology
ISSN: 0099-2240
NlmUniqueID: 7605801
Country: United States
Language: English
Volume: 66
Issue: 11
Pages: 5030-5034

Researcher Affiliations

Yuki, N
  • Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186-8650, Kagoshima 899-8313, Japan.
Shimazaki, T
    Kushiro, A
      Watanabe, K
        Uchida, K
          Yuyama, T
            Morotomi, M

              MeSH Terms

              • Animals
              • Bacterial Adhesion
              • DNA, Bacterial / analysis
              • DNA, Bacterial / genetics
              • DNA, Ribosomal / analysis
              • DNA, Ribosomal / genetics
              • Epithelial Cells / microbiology
              • Female
              • Gastric Mucosa / microbiology
              • Genes, Bacterial
              • Genes, rRNA
              • Germ-Free Life
              • Horses / microbiology
              • Lactobacillus / classification
              • Lactobacillus / growth & development
              • Lactobacillus / isolation & purification
              • Lactobacillus / physiology
              • Nucleic Acid Hybridization
              • RNA, Ribosomal, 16S / genetics
              • Rats
              • Rats, Inbred F344
              • Sequence Analysis, DNA

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              This article has been cited 26 times.
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