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Journal of veterinary internal medicine2020; 34(6); 2727-2737; doi: 10.1111/jvim.15937

Effect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses.

Abstract: Omeprazole administration is associated with changes in gastric and fecal microbiota and increased incidence of Clostridioides difficile enterocolitis in humans and dogs. Objective: Study purpose was to assess the effect of omeprazole on gastric glandular and fecal microbiota in healthy adult horses. Methods: Eight healthy horses stabled on straw and fed 100% haylage. Methods: Prospective controlled study. Transendoscopic gastric glandular biopsies, gastric fluid, and fecal samples were obtained from each horse twice at a 7-day interval before the administration of omeprazole. Samples were taken on the same horses before and after a 7-day administration of omeprazole (4 mg/kg PO q24h). pH was assessed on fresh gastric fluid and other samples were kept at -20°C until analysis. Bacterial taxonomy profiling was obtained by V1V3 16S amplicon sequencing from feces and gastric glandular biopsies. Analysis of alpha, beta diversity, and comparison between time points were performed with MOTHUR and results were considered significant when P < .05. Results: Gastric pH increased significantly after 7 days of omeprazole administration (P = .006). Omeprazole did not induce significant major changes in composition of fecal or gastric glandular microbiota, however, after administration, certain microbial genera became more predominant in the gastric glandular mucosa (lower Simpson's evenness, P = .05). Only the genus Clostridium sensu strictu_1 had a significant shift in the glandular gastric mucosa after omeprazole administration (P = .002). No population shifts were observed in feces. Conclusions: Oral administration of omeprazole could have fewer effects in gastrointestinal microbiota in the horse compared to other species.
© 2020 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of American College of Veterinary Internal Medicine.
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Publication Date: 2020-10-16 PubMed ID: 33063923PubMed Central: PMC7694827DOI: 10.1111/jvim.15937Google Scholar: Lookup
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Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research article outlines a study conducted to investigate the impact of omeprazole, a medication used to reduce stomach acid production, on the gastric and fecal microbiota in healthy horses. The study found that while omeprazole increased gastric pH levels, major shifts in microbiota composition were not observed, indicating it may have fewer gastro-intestinal effects in horses than in other species.

Study Objective and Methodology

  • This research was an investigation into the impact of orally administered omeprazole on the composition of gastric glandular and fecal microbiota in healthy adult horses.
  • Eight healthy horses, all housed in stables with a diet of 100% haylage, were studied. The research was intended to observe effects over time, and samples were collected at two time points – before and after omeprazole administration.
  • Omeprazole was administered at a dosage of 4 mg/kg once every 24 hours over a 7-day period.
  • Several types of samples – including gastric fluid and glandular biopsies, and fecal matter – were collected from each horse using trans-endoscopic methods. The pH of the fresh gastric fluid was also recorded.
  • All collected samples were stored at a temperature of -20°C until further analysis.
  • Bacterial taxonomy profiles were obtained from the feces and gastric glandular biopsy samples by means of V1V3 16S amplicon sequencing, a common method for characterizing microbial communities.

Results of the Study

  • The study resulted in a significant increase in gastric pH after a 7-day period of omeprazole administration.
  • Notwithstanding the pH increase, no significant major shifts in fecal or gastric glandular microbiota composition were observed. However, certain microbial genera, particularly the Clostridium sensu strictu_1 genus, had an increased presence in the gastric glandular mucosa after the administration of omeprazole.
  • The study observed no significant shifts in the fecal bacterial populations as a result of the omeprazole administration.

Conclusions Derived from the Study

  • The research concluded that oral administration of omeprazole could potentially have less impact on gastrointestinal microbiota in horses than in other species. This conclusion is primarily based on the observation that although the drug did alter gastric pH levels, it didn’t lead to any major shifts in the composition of fecal or gastric glandular microbiota.
  • This might underline potential differences between species in how omeprazole interacts with gastrointestinal systems, and could have implications for how medications are tailored to suit different animals.

Cite This Article

APA
Cerri S, Taminiau B, de Lusancay AH, Lecoq L, Amory H, Daube G, Cesarini C. (2020). Effect of oral administration of omeprazole on the microbiota of the gastric glandular mucosa and feces of healthy horses. J Vet Intern Med, 34(6), 2727-2737. https://doi.org/10.1111/jvim.15937

Publication

Journal of veterinary internal medicine
ISSN: 1939-1676
NlmUniqueID: 8708660
Country: United States
Language: English
Volume: 34
Issue: 6
Pages: 2727-2737

Researcher Affiliations

Cerri, Simona
  • FARAH Center, Comparative Veterinary Medicine Section, Clinical Department of Companion Animals and Equids, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
Taminiau, Bernard
  • FARAH Center, Veterinary Public Health Section, Department of Food Sciences - Microbiology, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
de Lusancay, Alexandra Hache-Carré
  • FARAH Center, Comparative Veterinary Medicine Section, Clinical Department of Companion Animals and Equids, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
Lecoq, Laureline
  • FARAH Center, Comparative Veterinary Medicine Section, Clinical Department of Companion Animals and Equids, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
Amory, Hélène
  • FARAH Center, Comparative Veterinary Medicine Section, Clinical Department of Companion Animals and Equids, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
Daube, Georges
  • FARAH Center, Veterinary Public Health Section, Department of Food Sciences - Microbiology, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.
Cesarini, Carla
  • FARAH Center, Comparative Veterinary Medicine Section, Clinical Department of Companion Animals and Equids, Faculty of Veterinary Medicine, University of Liege, Liège, Belgium.

MeSH Terms

  • Administration, Oral
  • Animals
  • Dog Diseases
  • Dogs
  • Feces
  • Gastric Mucosa
  • Horse Diseases / drug therapy
  • Horses
  • Microbiota
  • Omeprazole / pharmacology
  • Omeprazole / therapeutic use
  • Prospective Studies
  • Stomach Ulcer / drug therapy
  • Stomach Ulcer / veterinary

Conflict of Interest Statement

Authors declare no conflict of interest.

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Citations

This article has been cited 9 times.
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