Comparative Characterization of Human and Equine Mesenchymal Stromal Cells: A Basis for Translational Studies in the Equine Model.
Abstract: Multipotent mesenchymal stromal cells (MSCs) have gained tremendous attention as potential therapeutic agents for the treatment of orthopedic diseases. Promising results have been obtained after application of MSCs for treatment of tendon and joint disease in the equine model, making it appear favorable to use these results as a basis for the translational process of the therapy. However, while the horse is considered a highly suitable model for orthopedic diseases, knowledge is lacking regarding the level of analogy of equine MSCs and their human counterparts. Therefore, the aim of this study was to assess the properties of human and equine adipose- and tendon-derived MSCs in a direct comparison. Basic properties of human and equine MSCs from both tissues were similar. The cells expressed CD29, CD44, CD90, and CD105 and lacked expression of CD73, CD14, CD34, CD45, CD79α, and MCHII/HLA-DR. No significant differences were found between proliferation potential of human and equine MSCs in early passages, but recovery of nucleated cells after tissue digestion as well as proliferation in later passages was higher in equine samples (p < 0.01). All samples showed a good migration capacity and multilineage differentiation potential. However, while osteogenic differentiation was achieved in all equine samples, it was only evident in five out of nine human tendon-derived samples. Human MSCs further showed a higher expression of collagen IIIA1 and tenascin-C, but lower expression of decorin and scleraxis (p < 0.01). Although revealing some potentially relevant differences, the study demonstrates a high level of analogy between human and equine MSCs, providing a basis for translational research in the equine model according to the guidelines issued by the authorities.
Publication Date: 2015-04-07 PubMed ID: 25853993DOI: 10.3727/096368915X687822Google Scholar: Lookup
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- Comparative Study
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The research article focuses on the study of Mesenchymal Stromal Cells (MSCs) derived from humans and horses, comparing their basic properties to establish a basis for translational studies in equine models. The study showed significant similarities between human and equine MSCs, despite some notable differences.
Objectives of the Study
- The main aim of the study was to determine and compare the basic properties of human and equine MSCs derived from adipose (fat) and tendon tissues.
- This assessment was done to fill the existing gap in knowledge regarding how similar or different equine MSCs are from their human counterparts.
- The study aimed at establishing a sound basis for using equine models in translational research, especially in relation to orthopedic diseases.
Methodology and Findings
- Human and equine MSCs from adipose and tendon tissues were compared directly in this study.
- Both MSC types expressed CD29, CD44, CD90, and CD105 and didn’t show an expression of CD73, CD14, CD34, CD45, CD79α, and MCHII/HLA-DR. These characteristics are significant because they suggest a certain level of similarity between the two types of MSCs.
- Recovery of nucleated cells after tissue digestion and proliferation in later passages was found to be higher in equine samples.
- All samples from both human and equine MSCs showed good migration capacity and multilineage differentiation potential, indicating basic function similarity.
- Human MSCs displayed a higher expression of collagen IIIA1 and tenascin-C, but a lower expression of decorin and scleraxis. On the other hand, osteogenic differentiation was achieved in all equine samples but only in some human tendon-derived samples. These findings reveal the identified differences between the two types of MSCs.
Implications of the Study
- Despite some potentially relevant differences between human and equine MSCs, a high level of similarity exists between them. This is significant for future research, as it means that findings from studies using equine models can have translational relevance to human MSCs, particularly in the treatment of orthopedic diseases.
- These findings pave the way for more focused translational research using equine models, following guidelines issued by authorities for such studies.
Cite This Article
APA
Hillmann A, Ahrberg AB, Brehm W, Heller S, Josten C, Paebst F, Burk J.
(2015).
Comparative Characterization of Human and Equine Mesenchymal Stromal Cells: A Basis for Translational Studies in the Equine Model.
Cell Transplant, 25(1), 109-124.
https://doi.org/10.3727/096368915X687822 Publication
Researcher Affiliations
- Translational Centre for Regenerative Medicine (TRM), University of Leipzig, Leipzig, Germany.
MeSH Terms
- Adult
- Animals
- Biomarkers / metabolism
- Cell Differentiation
- Cell Lineage
- Cell Movement
- Cell Proliferation
- Female
- Horses
- Humans
- Immunophenotyping
- Male
- Mesenchymal Stem Cells / cytology
- Middle Aged
- Models, Animal
- Real-Time Polymerase Chain Reaction
- Tendons / metabolism
- Translational Research, Biomedical
- Young Adult
Citations
This article has been cited 18 times.- Stage HJ, Trappe S, Söllig K, Trachsel DS, Kirsch K, Zieger C, Merle R, Aschenbach JR, Gehlen H. Multilineage Differentiation Potential of Equine Adipose-Derived Stromal/Stem Cells from Different Sources.. Animals (Basel) 2023 Apr 15;13(8).
- Heyman E, Meeremans M, Devriendt B, Olenic M, Chiers K, De Schauwer C. Validation of a color deconvolution method to quantify MSC tri-lineage differentiation across species.. Front Vet Sci 2022;9:987045.
- Trachsel DS, Stage HJ, Rausch S, Trappe S, Söllig K, Sponder G, Merle R, Aschenbach JR, Gehlen H. Comparison of Sources and Methods for the Isolation of Equine Adipose Tissue-Derived Stromal/Stem Cells and Preliminary Results on Their Reaction to Incubation with 5-Azacytidine.. Animals (Basel) 2022 Aug 11;12(16).
- Schnabel CL, Fletemeyer B, Lübke S, Marti E, Wagner B, Alber G. CD154 Expression Indicates T Cell Activation Following Tetanus Toxoid Vaccination of Horses.. Front Immunol 2022;13:805026.
- Burk J, Melzer M, Hagen A, Lips KS, Trinkaus K, Nimptsch A, Leopold J. Phospholipid Profiles for Phenotypic Characterization of Adipose-Derived Multipotent Mesenchymal Stromal Cells.. Front Cell Dev Biol 2021;9:784405.
- Hagen A, Lehmann H, Aurich S, Bauer N, Melzer M, Moellerberndt J, Patané V, Schnabel CL, Burk J. Scalable Production of Equine Platelet Lysate for Multipotent Mesenchymal Stromal Cell Culture.. Front Bioeng Biotechnol 2020;8:613621.
- Theerakittayakorn K, Thi Nguyen H, Musika J, Kunkanjanawan H, Imsoonthornruksa S, Somredngan S, Ketudat-Cairns M, Parnpai R. Differentiation Induction of Human Stem Cells for Corneal Epithelial Regeneration.. Int J Mol Sci 2020 Oct 22;21(21).
- Bukowska J, Szóstek-Mioduchowska AZ, Kopcewicz M, Walendzik K, Machcińska S, Gawrońska-Kozak B. Adipose-Derived Stromal/Stem Cells from Large Animal Models: from Basic to Applied Science.. Stem Cell Rev Rep 2021 Jun;17(3):719-738.
- Kim KH, Park TS, Cho BW, Kim TM. Nanoparticles from Equine Fetal Bone Marrow-Derived Cells Enhance the Survival of Injured Chondrocytes.. Animals (Basel) 2020 Sep 23;10(10).
- Horstmeier C, Ahrberg AB, Berner D, Burk J, Gittel C, Hillmann A, Offhaus J, Brehm W. In Vivo Magic Angle Magnetic Resonance Imaging for Cell Tracking in Equine Low-Field MRI.. Stem Cells Int 2019;2019:5670106.
- Gugjoo MB, Fazili MR, Gayas MA, Ahmad RA, Dhama K. Animal mesenchymal stem cell research in cartilage regenerative medicine - a review.. Vet Q 2019 Dec;39(1):95-120.
- Hillmann A, Paebst F, Brehm W, Piehler D, Schubert S, Tárnok A, Burk J. A novel direct co-culture assay analyzed by multicolor flow cytometry reveals context- and cell type-specific immunomodulatory effects of equine mesenchymal stromal cells.. PLoS One 2019;14(6):e0218949.
- Shojaee A, Parham A. Strategies of tenogenic differentiation of equine stem cells for tendon repair: current status and challenges.. Stem Cell Res Ther 2019 Jun 18;10(1):181.
- Brandt L, Schubert S, Scheibe P, Brehm W, Franzen J, Gross C, Burk J. Tenogenic Properties of Mesenchymal Progenitor Cells Are Compromised in an Inflammatory Environment.. Int J Mol Sci 2018 Aug 28;19(9).
- Ahrberg AB, Horstmeier C, Berner D, Brehm W, Gittel C, Hillmann A, Josten C, Rossi G, Schubert S, Winter K, Burk J. Effects of mesenchymal stromal cells versus serum on tendon healing in a controlled experimental trial in an equine model.. BMC Musculoskelet Disord 2018 Jul 18;19(1):230.
- Desancé M, Contentin R, Bertoni L, Gomez-Leduc T, Branly T, Jacquet S, Betsch JM, Batho A, Legendre F, Audigié F, Galéra P, Demoor M. Chondrogenic Differentiation of Defined Equine Mesenchymal Stem Cells Derived from Umbilical Cord Blood for Use in Cartilage Repair Therapy.. Int J Mol Sci 2018 Feb 10;19(2).
- Veron AD, Bienboire-Frosini C, Feron F, Codecasa E, Deveze A, Royer D, Watelet P, Asproni P, Sadelli K, Chabaud C, Stamegna JC, Fagot J, Khrestchatisky M, Cozzi A, Roman FS, Pageat P, Mengoli M, Girard SD. Isolation and characterization of olfactory ecto-mesenchymal stem cells from eight mammalian genera.. BMC Vet Res 2018 Jan 17;14(1):17.
- Branly T, Bertoni L, Contentin R, Rakic R, Gomez-Leduc T, Desancé M, Hervieu M, Legendre F, Jacquet S, Audigié F, Denoix JM, Demoor M, Galéra P. Characterization and use of Equine Bone Marrow Mesenchymal Stem Cells in Equine Cartilage Engineering. Study of their Hyaline Cartilage Forming Potential when Cultured under Hypoxia within a Biomaterial in the Presence of BMP-2 and TGF-ß1.. Stem Cell Rev Rep 2017 Oct;13(5):611-630.
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