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The Histochemical journal1993; 25(4); 304-311; doi: 10.1007/BF00159122

Comparative immunohistolocalization of carbonic anhydrase isozymes I, II and III in the equine and bovine digestive tract.

Abstract: Immunohistochemical localizations of carbonic anhydrase isozymes (CA-I, CA-II and CA-III) in equine and bovine digestive tracts were studied. In the horse, epithelial cells in both the oesophagus and non-glandular part of the stomach lacked all three isozymes. In contrast, surface epithelial and parietal cells in the glandular region of the stomach showed reactivity for CA-II. In the small intestine, absorptive columnar cells covering the villi in the duodenum were positive for CA-II. The epithelium of the jejunum and ileum lacked all three isozymes. In the large intestine, CA-II was detected in the columnar cells in the upper part of the crypt. In cattle, epithelial cells of the oesophagus showed reactions for CA-I and CA-III but not for CA-II. Although the absorptive epithelial cells of the small intestine lacked CA-I, CA-II and CA-III, those of the upper part of large intestine crypts were heavily stained for all three isozymes.
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Publication Date: 1993-04-01 PubMed ID: 8491671DOI: 10.1007/BF00159122Google Scholar: Lookup
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  • Comparative Study
  • Journal Article

Summary

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This research article investigates the presence and distribution of different types of carbonic anhydrase (CA-I, CA-II, and CA-III) in the digestive systems of horses and cows.

Understanding Carbonic Anhydrase Isozymes

  • These enzymes are proteins that catalyze or speed up chemical reactions in our bodies.
  • Carbonic anhydrases, in particular, catalyze the conversion of carbon dioxide and water into carbonic acid, which can then dissociate to yield a bicarbonate ion and a proton.
  • The research specifically looks at three isozymes of the carbonic anhydrase enzyme, CA-I, CA-II, and CA-III. Isozymes are enzymes that vary in amino acid sequence but catalyze the same chemical reaction.

Findings in the Equine Digestive Tract

  • The research found that epithelial cells in the oesophagus and non-glandular part of the horse’s stomach did not contain any of the three isozymes.
  • However, in the glandular part of the stomach, these isozymes were detected in the surface epithelial cells and parietal cells.
  • In the small intestine, the absorptive columnar cells showed the presence of CA-II, specifically in the duodenum part. However, the other segments of small intestine like the jejunum and ileum lacked all three isozymes.
  • In the large intestine, CA-II turned up in the columnar cells in the upper part of the crypt.

Findings in the Bovine Digestive Tract

  • In cattle’s esophagus, there were reactions for CA-I and CA-III, but not for CA-II.
  • The absorptive epithelial cells in the small intestine didn’t have any isozyme, much like the results from the jejunum and ileum in horses.
  • Interestingly, the epithelial cells in the upper parts of the large intestine crypts showed the presence of all three isozymes.

Significance of Findings

  • This study provides valuable insights into the distribution of these enzymes in the digestive tract of these animals, which may give scientists an understanding of these enzymes’ roles in different parts of the animal’s digestive system.
  • The varied presence of these enzymes can influence how different animals digest and absorb nutrients, leading to differences in nutrition and overall health.

Cite This Article

APA
Sasaki K, Igarashi S, Amasaki T, Amasaki H, Nishita T, Kano Y, Asari M. (1993). Comparative immunohistolocalization of carbonic anhydrase isozymes I, II and III in the equine and bovine digestive tract. Histochem J, 25(4), 304-311. https://doi.org/10.1007/BF00159122

Publication

The Histochemical journal
ISSN: 0018-2214
NlmUniqueID: 0163161
Country: Netherlands
Language: English
Volume: 25
Issue: 4
Pages: 304-311

Researcher Affiliations

Sasaki, K
  • Department of Anatomy I, Azabu University School of Veterinary Medicine, Kanagawa, Japan.
Igarashi, S
    Amasaki, T
      Amasaki, H
        Nishita, T
          Kano, Y
            Asari, M

              MeSH Terms

              • Animals
              • Antibody Specificity
              • Carbonic Anhydrases / analysis
              • Cattle / metabolism
              • Digestive System / enzymology
              • Electrophoresis, Polyacrylamide Gel
              • Female
              • Horses / metabolism
              • Immunoenzyme Techniques
              • Isoenzymes / analysis

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              Citations

              This article has been cited 1 times.
              1. Nishita T, Anezaki R, Matsunaga K, Orito K, Kasuya T, Sakanoue H, Matsunaga A, Arishima K. Measurement of Carbonic Anhydrase I and II Isozymes in Feces as a Marker of Occult Blood in Horses with Intestinal Tract Bleeding. J Equine Sci 2013;24(4):57-62.
                doi: 10.1294/jes.24.57pubmed: 24834003google scholar: lookup
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