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Comparative immunolocalization of heat shock proteins (Hsp)-60, -70, -90 in boar, stallion, dog and cat spermatozoa.
Abstract: Heat shock proteins (Hsp)-60, -70 and -90 are important testis chaperones that fulfil several functions during sperm cell maturation. In post-meiotic cells, their expression may change or may be undetectable and in some species it may be evident in mature spermatozoa. The aims of this study were to verify whether Hsp60, -70 and -90 are present in the sperm, and to compare their localization in boar, stallion, cat and dog spermatozoa by immunofluorescence. Hsp-60 immunoreactivity was detected in sperm midpiece in all the species examined. In stallion sperm, Hsp70 signal was localized in the sub-equatorial band, whereas immunoreactivity was evident on the neck of dog spermatozoa and on both neck and sub-equatorial region of cat spermatozoa. In agreement with our previous observations, a triangular fluorescent signal in the equatorial segment of fresh boar sperm was detected. Hsp90 immunoreactivity was present in different portions of sperm tail: in the midpiece of both boar and cat spermatozoa and in the neck and throughout the tail in dog and stallion spermatozoa, respectively. When capacitation and acrosome reaction were induced in boar, stallion and dog spermatozoa, no changes in both Hsp60 and -90 were recorded by either Western blot or immunofluorescence. After induction of acrosome reaction, a Hsp70 redistribution in boar spermatozoa and an increased percentage of stallion spermatozoa showing the post-acrosomal signal were observed although no changes were recorded by Western blot; in dog spermatozoa, no changes in Hsp70 were found by Western blot and immunofluorescence after capacitation and acrosome reaction.
Publication Date: 2008-01-23 PubMed ID: 18226022DOI: 10.1111/j.1439-0531.2007.00918.xGoogle Scholar: Lookup The Equine Research Bank provides access to a large database of publicly available scientific literature. Inclusion in the Research Bank does not imply endorsement of study methods or findings by Mad Barn.
- Journal Article
- Research Support
- Non-U.S. Gov't
Summary
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The study investigates the presence and location of heat shock proteins (Hsp)-60, -70 and -90 in the sperm of boars, stallions, cats, and dogs, using the technique of immunofluorescence. These proteins, which are crucial for sperm cell maturation, were found in different areas in the sperm of the various species. The researchers also observed their behavior during capacitation and the acrosome reaction.
Role of Heat Shock Proteins in Sperm Maturation
- Heat Shock Proteins (Hsp)-60, -70, and -90 serve as testis chaperones. They play significant roles in the maturation process of sperm cells.
- During the post-meiotic stage, the expression of these proteins may change. In some instances, they become undetectable. Meanwhile, in certain species, these proteins can be detected even in mature spermatozoa.
Localization of Heat Shock Proteins in Sperm
- The study aimed to identify two things: whether these proteins exist in sperm and their locations in the sperm cells of boars, stallions, cats, and dogs.
- An immunofluorescence technique was applied to achieve these objectives. The results show that Hsp-60 is found in the sperm midpiece of all the examined species.
- The Hsp70 signal was observed in different areas, depending on the species: it was located in the sub-equatorial band in stallion sperm, on the neck of dog spermatozoa, and both on the neck and sub-equatorial region of cat spermatozoa.
- Meanwhile, Hsp90 was present in different parts of the sperm tail: the midpiece of boar and cat spermatozoa, and the neck and throughout the tail in dog and stallion spermatozoa respectively.
Behavior of Heat Shock Proteins during Capacitation and Acrosome Reaction
- The researchers conducted further examination to understand how Hsp-60, -70 and -90 behave during capacitation and the acrosome reaction. It was found the locations of both Hsp60 and -90 remained unchanged in boar, stallion, and dog spermatozoa as revealed by either Western blot or immunofluorescence.
- However, after inducing the acrosome reaction, the researchers noted a Hsp70 redistribution in boar spermatozoa and an uptick in the percentage of stallion spermatozoa showing the post-acrosomal signal.
- In dog spermatozoa, no changes were found in Hsp70 after capacitation and acrosome reaction, as per the findings from using Western blot and immunofluorescence.
Cite This Article
APA
Volpe S, Galeati G, Bernardini C, Tamanini C, Mari G, Zambelli D, Seren E, Spinaci M.
(2008).
Comparative immunolocalization of heat shock proteins (Hsp)-60, -70, -90 in boar, stallion, dog and cat spermatozoa.
Reprod Domest Anim, 43(4), 385-392.
https://doi.org/10.1111/j.1439-0531.2007.00918.x Publication
Researcher Affiliations
- Dipartimento di Morfofisiologia Veterinaria e Produzioni Animali (DIMORFIPA), Università di Bologna, Bologna, Italy.
MeSH Terms
- Acrosome Reaction / physiology
- Animals
- Blotting, Western / veterinary
- Cats
- Chaperonin 60 / metabolism
- Dogs
- Fluorescent Antibody Technique, Indirect / veterinary
- HSP70 Heat-Shock Proteins / metabolism
- HSP90 Heat-Shock Proteins / metabolism
- Horses
- Male
- Species Specificity
- Sperm Capacitation / physiology
- Spermatogenesis / physiology
- Spermatozoa / cytology
- Spermatozoa / metabolism
- Swine
- Tissue Distribution
Citations
This article has been cited 16 times.- Gouletsou PG, Tsangaris GT, Katsarou EI, Bourganou MV, Barbagianni MS, Venianaki AP, Bouroutzika E, Anagnostopoulos AK, Fthenakis GC, Katsafadou AI. Proteomics Evaluation of Semen of Clinically Healthy Beagle-Breed Dogs. Vet Sci 2022 Dec 15;9(12).
- Rosyada ZNA, Ulum MF, Tumbelaka LITA, Solihin DD, Purwantara B, Memili E. Implications of sperm heat shock protein 70-2 in bull fertility. Vet World 2022 Jun;15(6):1456-1466.
- Grassi S, Bisconti M, Martinet B, Arcolia V, Simon JF, Wattiez R, Leroy B, Hennebert E. Targeted Analysis of HSP70 Isoforms in Human Spermatozoa in the Context of Capacitation and Motility. Int J Mol Sci 2022 Jun 10;23(12).
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- Abdi Z, Mohsenzadeh S, Jafari Anarkooli I, Ahmadi A, Ghorbanlou M, Arianmanesh M. THE EFFECT OF HSP60 ON FERTILIZATION AND PRE-IMPLANTATION EMBRYO DEVELOPMENT IN MICE: AN IN VITRO STUDY. Acta Endocrinol (Buchar) 2019 Apr-Jun;15(2):153-157.
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- Sagare-Patil V, Bhilawadikar R, Galvankar M, Zaveri K, Hinduja I, Modi D. Progesterone requires heat shock protein 90 (HSP90) in human sperm to regulate motility and acrosome reaction. J Assist Reprod Genet 2017 Apr;34(4):495-503.
- Li K, Xue Y, Chen A, Jiang Y, Xie H, Shi Q, Zhang S, Ni Y. Heat shock protein 90 has roles in intracellular calcium homeostasis, protein tyrosine phosphorylation regulation, and progesterone-responsive sperm function in human sperm. PLoS One 2014;9(12):e115841.
- Meccariello R, Chianese R, Ciaramella V, Fasano S, Pierantoni R. Molecular chaperones, cochaperones, and ubiquitination/deubiquitination system: involvement in the production of high quality spermatozoa. Biomed Res Int 2014;2014:561426.
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- Kameshwari DB, Bhande S, Sundaram CS, Kota V, Siva AB, Shivaji S. Glucose-regulated protein precursor (GRP78) and tumor rejection antigen (GP96) are unique to hamster caput epididymal spermatozoa. Asian J Androl 2010 May;12(3):344-55.
- Barranco I, Alvarez-Barrientos A, Parra A, Martínez-Díaz P, Lucas X, Roca J. Immunophenotype profile by flow cytometry reveals different subtypes of extracellular vesicles in porcine seminal plasma. Cell Commun Signal 2024 Jan 23;22(1):63.
- Shakeel M, Yoon M. Heat stress and stallion fertility. J Anim Sci Technol 2023 Jul;65(4):683-697.
- Li J, Zhao W, Zhu J, Wang S, Ju H, Chen S, Basioura A, Ferreira-Dias G, Liu Z. Temperature Elevation during Semen Delivery Deteriorates Boar Sperm Quality by Promoting Apoptosis. Animals (Basel) 2023 Oct 13;13(20).
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