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Home / Equine Research Bank / Vet Parasitol / Comparative serological diagnosis of toxoplasmosis in horses...
Veterinary parasitology2006; 145(1-2); 31-36; doi: 10.1016/j.vetpar.2006.11.010

Comparative serological diagnosis of toxoplasmosis in horses using locally isolated Toxoplasma gondii.

Abstract: A total of 420 serum samples collected from horses of different ages, sexes and breeds, located at some horse farms in Egypt, were used for serological studies. A crude antigen of the locally isolated Toxoplasma gondii tachyzoites from horse tissues (LA) was used for the detection of T. gondii antibodies in horses. It showed good diagnostic efficiency (38.1%) by Enzyme Linked Immunosorbent Assay (ELISA). To increase this efficiency, an affinity purification process was performed. Two fractions were obtained from LA by CNBr-Sepharose 4B affinity column chromatography named; unbound (LAunb) and bound (LAb). LAb showed the highest diagnostic potency (51.7%), while LAunb showed the lowest value (31.7%) using ELISA. The electrophoretic profile of LA (12 bands), LAb (6 bands) and LAunb (6 bands) showed molecular weights ranged from 25.1 to 184.3kDa. The immunoreactive bands of each of the three antigens were identified with infected horse sera by immunoblot assay. Four immunogenic bands of 155.8, 115.1, 83.2 and 66.2kDa were identified in LAb and probably were responsible for the highest diagnostic potency. Examination of horse sera by Indirect Fluorescence Antibody Test (IFAT) at a dilution of 1: 64 and Modified Agglutination Test (MAT) at a dilution of 1: 25 revealed that 170 (40.5%) and 202 (48.1%) had antibodies against T. gondii, respectively. The current research introduces crude and purified fractions (bound and unbound) obtained from the locally isolated tachyzoites (equine origin), which are utilized globally for the first time in detection of T. gondii antibodies in horses. Furthermore, this study recommended utilization of the bound fraction in diagnosis of toxoplasmosis using indirect ELISA which proved better diagnostic potency compared with IFAT and MAT.
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Publication Date: 2006-12-14 PubMed ID: 17174034DOI: 10.1016/j.vetpar.2006.11.010Google Scholar: Lookup
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  • Comparative Study
  • Evaluation Study
  • Journal Article

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The study evaluated a more effective method for diagnosing toxoplasmosis in horses by using a crude antigen of the locally isolated Toxoplasma gondii. The method showed a high diagnostic efficacy, particularly when applying an affinity purification process.

Sample Collection and Testing

  • Altogether, 420 serum samples were collected from different horses of varying ages, sexes, and breeds from horse farms in Egypt. These samples were used for serological tests.
  • To detect T. gondii antibodies in the horses, they used a crude antigen of the locally isolated T. gondii tachyzoites sourced from horse tissues.
  • They verified the efficiency of the crude antigen through an Enzyme Linked Immunosorbent Assay (ELISA) test, which displayed a good diagnostic efficiency of 38.1%.

Increasing Testing Efficiency

  • To enhance this efficiency, researchers implemented an affinity purification process and obtained two fractions from the original horse antigen. They named these fractions as unbound (LAunb) and bound (LAb).
  • LAb demonstrated the superior diagnostic potency, with a value of 51.7 %. In contrast, LAunb showed the least diagnostic potency, with a value of 31.7 %.

Electrophoretic Profile and Immunogenic Bands

  • The electrophoretic profile demonstrated that the LA contained 12 bands, LAb 6 bands, and LAunb also 6 bands. These bands showed molecular weights ranging from 25.1 to 184.3kDa.
  • Four immunogenic bands of 155.8, 115.1, 83.2, and 66.2kDa were identified in LAb and were thought to be responsible for its higher diagnostic potency.

Comparison with Other Tests

  • The Indirect Fluorescence Antibody Test (IFAT) and Modified Agglutination Test (MAT) were also used to test the horse sera. IFAT at a dilution of 1:64 revealed that 170 (40.5 %) horses had antibodies against T. gondii.
  • Using the MAT at a dilution of 1:25, they found that 202 (48.1 %) horses had antibodies against T. gondii.
  • The results of these two tests were lower than that obtained using the LAb fraction, demonstrating its higher potency.

Conclusion

  • The research presents the first global use of crude and purified fractions obtained from the locally isolated tachyzoites of equine origin for detecting T. gondii antibodies in horses.
  • Finally, the study recommended the use of the bound fraction in diagnosing toxoplasmosis using indirect ELISA, which proved to have better diagnostic potency than IFAT and MAT.

Cite This Article

APA
Ghazy AA, Shaapan RM, Abdel-Rahman EH. (2006). Comparative serological diagnosis of toxoplasmosis in horses using locally isolated Toxoplasma gondii. Vet Parasitol, 145(1-2), 31-36. https://doi.org/10.1016/j.vetpar.2006.11.010

Publication

Veterinary parasitology
ISSN: 0304-4017
NlmUniqueID: 7602745
Country: Netherlands
Language: English
Volume: 145
Issue: 1-2
Pages: 31-36

Researcher Affiliations

Ghazy, A A
  • Department of Parasitology and Animal Diseases, Veterinary Research Division, National Research Center, Dokki, Giza, Egypt. aaghazy7@hotmail.com
Shaapan, R M
    Abdel-Rahman, Eman H

      MeSH Terms

      • Agglutination Tests / methods
      • Agglutination Tests / veterinary
      • Animals
      • Antibodies, Protozoan / blood
      • Egypt
      • Enzyme-Linked Immunosorbent Assay / methods
      • Enzyme-Linked Immunosorbent Assay / veterinary
      • Female
      • Fluorescent Antibody Technique, Indirect / methods
      • Fluorescent Antibody Technique, Indirect / veterinary
      • Horse Diseases / blood
      • Horse Diseases / diagnosis
      • Horse Diseases / parasitology
      • Horses
      • Male
      • Toxoplasma / isolation & purification
      • Toxoplasmosis, Animal / blood
      • Toxoplasmosis, Animal / diagnosis
      • Toxoplasmosis, Animal / parasitology

      Citations

      This article has been cited 12 times.
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