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Home / Equine Research Bank / Osteoarthritis Cartilage / Comparative study of depth-dependent characteristics of equi...
Osteoarthritis and cartilage2012; 20(10); 1147-1151; doi: 10.1016/j.joca.2012.06.005

Comparative study of depth-dependent characteristics of equine and human osteochondral tissue from the medial and lateral femoral condyles.

Abstract: Articular cartilage defects are common after joint injuries. When left untreated, the biomechanical protective function of cartilage is gradually lost, making the joint more susceptible to further damage, causing progressive loss of joint function and eventually osteoarthritis (OA). In the process of translating promising tissue-engineering cartilage repair approaches from bench to bedside, pre-clinical animal models including mice, rabbits, goats, and horses, are widely used. The equine species is becoming an increasingly popular model for the in vivo evaluation of regenerative orthopaedic approaches. As there is also an increasing body of evidence suggesting that successful lasting tissue reconstruction requires an implant that mimics natural tissue organization, it is imperative that depth-dependent characteristics of equine osteochondral tissue are known, to assess to what extent they resemble those in humans. Therefore, osteochondral cores (4-8 mm) were obtained from the medial and lateral femoral condyles of equine and human donors. Cores were processed for histology and for biochemical quantification of DNA, glycosaminoglycan (GAG) and collagen content. Equine and human osteochondral tissues possess similar geometrical (thickness) and organizational (GAG, collagen and DNA distribution with depth) features. These comparable trends further underscore the validity of the equine model for the evaluation of regenerative approaches for articular cartilage.
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Publication Date: 2012-07-07 PubMed ID: 22781206DOI: 10.1016/j.joca.2012.06.005Google Scholar: Lookup
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  • Comparative Study
  • Journal Article
  • Research Support
  • Non-U.S. Gov't

Summary

This research summary has been generated with artificial intelligence and may contain errors and omissions. Refer to the original study to confirm details provided. Submit correction.

The research examines the similarities and characteristics of equine (horse) and human osteochondral tissue in a comparative study. The aim is to ascertain the validity of using equine models in testing techniques for repairing joint injuries that can lead to osteoarthritis.

Objective of the Study

  • The main goal of this study is to test the relevance and potential effectiveness of the equine model for testing regenerative orthopaedic treatments.
  • To conduct this research, the team extracted osteochondral cores from the medial and lateral femoral condyles of horses and human donors.

About Osteochondral Tissue

  • Osteochondral tissue refers to the combined area of bone (osteo) and cartilage (chondral) at the ends of the bones in a joint. It plays a crucial role in the smooth operation of the joint.
  • This tissue can suffer defects following joint injuries. If not treated timely, the condition may degenerate the protective function of cartilage, leading to a gradual loss of joint function, and eventually, osteoarthritis (OA).

Why Equine Model?

  • For the translation of tissue-engineering techniques from lab to patient use, several pre-clinical animal models are used such as mice, rabbits, goats, and horses.
  • The equine model is a popular choice for in vivo evaluation of regenerative orthopaedic techniques due to their anatomical and physiological similarities with humans.
  • Furthermore, to attain a successful and lasting tissue reconstruction, the implant must mimic the organization of natural tissue.
  • For this, an understanding of depth-dependent characteristics of equine osteochondral tissue is essential.

Findings

  • The study analyzed collected cores for histology and performed a biochemical quantification of DNA, glycosaminoglycan (GAG) and collagen content.
  • Results showed that both equine and human tissues have comparable geometric (thickness) and organizational (distribution of GAG, collagen, and DNA with depth) traits.
  • These similar trends underscore the validity of using an equine model for the testing and evaluation of regenerative techniques for articular cartilage issues.

Cite This Article

APA
Malda J, Benders KE, Klein TJ, de Grauw JC, Kik MJ, Hutmacher DW, Saris DB, van Weeren PR, Dhert WJ. (2012). Comparative study of depth-dependent characteristics of equine and human osteochondral tissue from the medial and lateral femoral condyles. Osteoarthritis Cartilage, 20(10), 1147-1151. https://doi.org/10.1016/j.joca.2012.06.005

Publication

Osteoarthritis and cartilage
ISSN: 1522-9653
NlmUniqueID: 9305697
Country: England
Language: English
Volume: 20
Issue: 10
Pages: 1147-1151

Researcher Affiliations

Malda, J
  • Department of Orthopaedics, University Medical Center Utrecht, The Netherlands. j.malda@umcutrecht.nl
Benders, K E M
    Klein, T J
      de Grauw, J C
        Kik, M J L
          Hutmacher, D W
            Saris, D B F
              van Weeren, P R
                Dhert, W J A

                  MeSH Terms

                  • Aged
                  • Animals
                  • Cartilage, Articular / anatomy & histology
                  • Cartilage, Articular / metabolism
                  • Collagen / metabolism
                  • DNA
                  • Femur / anatomy & histology
                  • Femur / metabolism
                  • Glycosaminoglycans / metabolism
                  • Horses / anatomy & histology
                  • Horses / physiology
                  • Humans
                  • Joints / anatomy & histology
                  • Joints / metabolism
                  • Models, Animal
                  • Species Specificity
                  • Tissue Engineering

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